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Nucleotide sequence of the virulence gene virG of the Agrobacterium tumefaciens octopine Ti plasmid: significant homology between virG and the regulatory genes ompR, phoB and dye of E. coli.
Nucleic Acids Res. 1986 Dec 22; 14(24):9933-42.NA

Abstract

The entire nucleotide sequence of the virG locus, from the octopine Ti plasmid of Agrobacterium tumefaciens strain 15955, has been determined. The virG gene is 801 nucleotides in length and has one open reading frame which encodes a protein of Mr 29,995. The virG gene is involved in the transcriptional activation of the Ti plasmid vir-loci, which occurs after induction by specific compounds present in plant exudate. Sequence analysis of the Agrobacterium virG protein showed significant homology with the Escherichia coli ompR, phoB and dye proteins, which are all positive regulatory genes for genes encoding envelope proteins. These results suggest that the virG gene encodes a positive regulatory protein which can activate vir gene expression.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3027669

Citation

Melchers, L S., et al. "Nucleotide Sequence of the Virulence Gene virG of the Agrobacterium Tumefaciens Octopine Ti Plasmid: Significant Homology Between virG and the Regulatory Genes ompR, phoB and Dye of E. Coli." Nucleic Acids Research, vol. 14, no. 24, 1986, pp. 9933-42.
Melchers LS, Thompson DV, Idler KB, et al. Nucleotide sequence of the virulence gene virG of the Agrobacterium tumefaciens octopine Ti plasmid: significant homology between virG and the regulatory genes ompR, phoB and dye of E. coli. Nucleic Acids Res. 1986;14(24):9933-42.
Melchers, L. S., Thompson, D. V., Idler, K. B., Schilperoort, R. A., & Hooykaas, P. J. (1986). Nucleotide sequence of the virulence gene virG of the Agrobacterium tumefaciens octopine Ti plasmid: significant homology between virG and the regulatory genes ompR, phoB and dye of E. coli. Nucleic Acids Research, 14(24), 9933-42.
Melchers LS, et al. Nucleotide Sequence of the Virulence Gene virG of the Agrobacterium Tumefaciens Octopine Ti Plasmid: Significant Homology Between virG and the Regulatory Genes ompR, phoB and Dye of E. Coli. Nucleic Acids Res. 1986 Dec 22;14(24):9933-42. PubMed PMID: 3027669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nucleotide sequence of the virulence gene virG of the Agrobacterium tumefaciens octopine Ti plasmid: significant homology between virG and the regulatory genes ompR, phoB and dye of E. coli. AU - Melchers,L S, AU - Thompson,D V, AU - Idler,K B, AU - Schilperoort,R A, AU - Hooykaas,P J, PY - 1986/12/22/pubmed PY - 1986/12/22/medline PY - 1986/12/22/entrez SP - 9933 EP - 42 JF - Nucleic acids research JO - Nucleic Acids Res. VL - 14 IS - 24 N2 - The entire nucleotide sequence of the virG locus, from the octopine Ti plasmid of Agrobacterium tumefaciens strain 15955, has been determined. The virG gene is 801 nucleotides in length and has one open reading frame which encodes a protein of Mr 29,995. The virG gene is involved in the transcriptional activation of the Ti plasmid vir-loci, which occurs after induction by specific compounds present in plant exudate. Sequence analysis of the Agrobacterium virG protein showed significant homology with the Escherichia coli ompR, phoB and dye proteins, which are all positive regulatory genes for genes encoding envelope proteins. These results suggest that the virG gene encodes a positive regulatory protein which can activate vir gene expression. SN - 0305-1048 UR - https://www.unboundmedicine.com/medline/citation/3027669/Nucleotide_sequence_of_the_virulence_gene_virG_of_the_Agrobacterium_tumefaciens_octopine_Ti_plasmid:_significant_homology_between_virG_and_the_regulatory_genes_ompR_phoB_and_dye_of_E__coli_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/14.24.9933 DB - PRIME DP - Unbound Medicine ER -