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Colorimetric adenosine aptasensor based on DNA cycling amplification and salt-induced aggregation of gold nanoparticles.
Mikrochim Acta. 2018 10 02; 185(10):488.MA

Abstract

An aptamer based assay is described for the colorimetric detection of adenosine. The presence of adenosine triggers the deformation of hairpin DNA oligonucleotide (HP1) containing adenosine aptamer and then hybridizes another unlabeled hairpin DNA oligonucleotide (HP2). This leads to the formation of a double strand with a blunt 3' terminal. After exonuclease III (Exo III)-assisted degradation, the guanine-rich strand (GRS) is released from HP2. Hence, the adenosine-HP1 complex is released to the solution where it can hybridize another HP2 and initiate many cycles of the digestion reaction with the assistance of Exo III. This leads to the generation of a large number of GRS strands after multiple cycles. The GRS stabilize the red AuNPs against aggregation in the presence of potassium ions. If, however, GRS forms a G-quadruplex, it loses its ability to protect gold nanoparticles (AuNPs) from salt-induced AuNP aggregation. Therefore, the color of the solution changes from red to blue which can be visually observed. This colorimetric assay has a 0.13 nM detection limit and a wide linear range that extends from 5 nM to 1 μM. Graphical abstract Schematic presentation of a colorimetric aptamer biosensor for adenosine detection based on DNA cycling amplification and salt-induced aggregation of gold nanoparticles.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kong C
Department of Chemistry, Capital Normal University, Beijing, 100048, China.
Gao L
College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao, 266590, China.
Chen Z
Department of Chemistry, Capital Normal University, Beijing, 100048, China. czb979216@sina.com.

MeSH

AdenosineAptamers, NucleotideBiosensing TechniquesColorimetryDNAGoldLimit of DetectionMetal NanoparticlesNucleic Acid Amplification TechniquesNucleic Acid HybridizationSalts

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30280258

Citation

Kong, Caiyun, et al. "Colorimetric Adenosine Aptasensor Based On DNA Cycling Amplification and Salt-induced Aggregation of Gold Nanoparticles." Mikrochimica Acta, vol. 185, no. 10, 2018, p. 488.
Kong C, Gao L, Chen Z. Colorimetric adenosine aptasensor based on DNA cycling amplification and salt-induced aggregation of gold nanoparticles. Mikrochim Acta. 2018;185(10):488.
Kong, C., Gao, L., & Chen, Z. (2018). Colorimetric adenosine aptasensor based on DNA cycling amplification and salt-induced aggregation of gold nanoparticles. Mikrochimica Acta, 185(10), 488. https://doi.org/10.1007/s00604-018-3031-z
Kong C, Gao L, Chen Z. Colorimetric Adenosine Aptasensor Based On DNA Cycling Amplification and Salt-induced Aggregation of Gold Nanoparticles. Mikrochim Acta. 2018 10 2;185(10):488. PubMed PMID: 30280258.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Colorimetric adenosine aptasensor based on DNA cycling amplification and salt-induced aggregation of gold nanoparticles. AU - Kong,Caiyun, AU - Gao,Linna, AU - Chen,Zhengbo, Y1 - 2018/10/02/ PY - 2018/09/07/received PY - 2018/09/27/accepted PY - 2018/10/4/entrez PY - 2018/10/4/pubmed PY - 2019/4/2/medline KW - Adenosine detecion KW - Color change KW - Colorimetric assay KW - DNA cycling amplification KW - Exonuclease III KW - G-quadruplex KW - Gold nanoparticle aggregation KW - Hairpin DNA oligonucleotide KW - Low detection limit KW - Wide linear range SP - 488 EP - 488 JF - Mikrochimica acta JO - Mikrochim Acta VL - 185 IS - 10 N2 - An aptamer based assay is described for the colorimetric detection of adenosine. The presence of adenosine triggers the deformation of hairpin DNA oligonucleotide (HP1) containing adenosine aptamer and then hybridizes another unlabeled hairpin DNA oligonucleotide (HP2). This leads to the formation of a double strand with a blunt 3' terminal. After exonuclease III (Exo III)-assisted degradation, the guanine-rich strand (GRS) is released from HP2. Hence, the adenosine-HP1 complex is released to the solution where it can hybridize another HP2 and initiate many cycles of the digestion reaction with the assistance of Exo III. This leads to the generation of a large number of GRS strands after multiple cycles. The GRS stabilize the red AuNPs against aggregation in the presence of potassium ions. If, however, GRS forms a G-quadruplex, it loses its ability to protect gold nanoparticles (AuNPs) from salt-induced AuNP aggregation. Therefore, the color of the solution changes from red to blue which can be visually observed. This colorimetric assay has a 0.13 nM detection limit and a wide linear range that extends from 5 nM to 1 μM. Graphical abstract Schematic presentation of a colorimetric aptamer biosensor for adenosine detection based on DNA cycling amplification and salt-induced aggregation of gold nanoparticles. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/30280258/Colorimetric_adenosine_aptasensor_based_on_DNA_cycling_amplification_and_salt_induced_aggregation_of_gold_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -