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Identification and Characterization of Orientia chuto in Trombiculid Chigger Mites Collected from Wild Rodents in Kenya.
J Clin Microbiol. 2018 12; 56(12)JC

Abstract

We present data that concurs with the reported geographical expansion of scrub typhus outside the "Tsutsugamushi Triangle" and addition of Orientia chuto as a second species in the Orientia genus. Wild rodents were caught in Marigat, Baringo County, Kenya, and ectoparasites, including chiggers, were recovered. Rodent and chigger species were identified by taxonomic features. DNA was extracted from the chiggers and used to amplify and/or sequence the 47-kDa high temperature transmembrane protein (TSA47), the 56-kDa type-specific antigen (TSA56), and the 16S rRNA (rrs) Orientia genes. The main rodent hosts identified were Acomys wilsoni, Crocidura sp., and Mastomys natalensis, which accounted for 59.2% of the total collection. Of these, A. wilsoni and M. natalensis harbored most of the chiggers that belonged to the Neotrombicula and Microtrombicula genera. A pool of chiggers from one of M. natalensis was positive for Orientia by TSA47 PCR, but Orientia did not amplify with the TSA56 primers. On sequencing the 850 bp of the TSA47 gene, the closest phylogenetic relative was O. chuto, with 97.65% sequence homology compared to 84.63 to 84.76% for O. tsutsugamushi 16S rRNA deep sequencing also revealed O. chuto as the closest phylogenetic relative, with 99.75% sequence homology. These results and the existing immunological and molecular reports are strongly suggestive of the existence of Orientia species in Kenya.

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Authors+Show Affiliations

U.S. Army Medical Research Directorate-Kenya/Kenya Medical Research Institute, Kisumu, Kenya.

U.S. Army Medical Directorate-Armed Forces Research Institute of Medical Science, Bangkok, Thailand.

U.S. Army Medical Research Directorate-Kenya/Kenya Medical Research Institute, Kisumu, Kenya.

U.S. Army Medical Research Directorate-Kenya/Kenya Medical Research Institute, Kisumu, Kenya.

U.S. Army Medical Directorate-Armed Forces Research Institute of Medical Science, Bangkok, Thailand.

U.S. Army Medical Research Directorate-Kenya/Kenya Medical Research Institute, Kisumu, Kenya.

U.S. Army Medical Research Directorate-Kenya/Kenya Medical Research Institute, Kisumu, Kenya.

U.S. Army Medical Directorate-Armed Forces Research Institute of Medical Science, Bangkok, Thailand.

U.S. Army Medical Research Directorate-Kenya/Kenya Medical Research Institute, Kisumu, Kenya.

U.S. Army Medical Directorate-Armed Forces Research Institute of Medical Science, Bangkok, Thailand.

U.S. Army Medical Research Directorate-Kenya/Kenya Medical Research Institute, Kisumu, Kenya john.waitumbi@usamru-k.org.

MeSH

AnimalsAnimals, WildBacterial ProteinsDNA, BacterialKenyaNucleic Acid HybridizationOrientia tsutsugamushiPhylogenyPolymerase Chain ReactionRNA, Ribosomal, 16SRickettsieaeRodent DiseasesRodentiaScrub TyphusSequence Analysis, DNATrombiculidae

Pub Type(s)

Journal Article

Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30282787

Citation

Masakhwe, Clement, et al. "Identification and Characterization of Orientia Chuto in Trombiculid Chigger Mites Collected From Wild Rodents in Kenya." Journal of Clinical Microbiology, vol. 56, no. 12, 2018.

Masakhwe C, Linsuwanon P, Kimita G, et al. Identification and Characterization of Orientia chuto in Trombiculid Chigger Mites Collected from Wild Rodents in Kenya. J Clin Microbiol. 2018;56(12).

Masakhwe, C., Linsuwanon, P., Kimita, G., Mutai, B., Leepitakrat, S., Yalwala, S., Abuom, D., Auysawasi, N., Gilbreath, T., Wanja, E., & Waitumbi, J. (2018). Identification and Characterization of Orientia chuto in Trombiculid Chigger Mites Collected from Wild Rodents in Kenya. Journal of Clinical Microbiology, 56(12). https://doi.org/10.1128/JCM.01124-18

Masakhwe C, et al. Identification and Characterization of Orientia Chuto in Trombiculid Chigger Mites Collected From Wild Rodents in Kenya. J Clin Microbiol. 2018;56(12) PubMed PMID: 30282787.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Identification and Characterization of Orientia chuto in Trombiculid Chigger Mites Collected from Wild Rodents in Kenya. AU - Masakhwe,Clement, AU - Linsuwanon,Piyada, AU - Kimita,Gathii, AU - Mutai,Beth, AU - Leepitakrat,Surachai, AU - Yalwala,Santos, AU - Abuom,David, AU - Auysawasi,Nutthanun, AU - Gilbreath,Tom, AU - Wanja,Elizabeth, AU - Waitumbi,John, Y1 - 2018/11/27/ PY - 2018/07/10/received PY - 2018/09/24/accepted PY - 2018/10/5/pubmed PY - 2019/9/7/medline PY - 2018/10/5/entrez KW - Neotrombicula and Microtrombicula chiggers KW - Orientia chuto KW - Orientia tsutsugamushi KW - chiggers KW - rodents KW - scrub typhus JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 56 IS - 12 N2 - We present data that concurs with the reported geographical expansion of scrub typhus outside the "Tsutsugamushi Triangle" and addition of Orientia chuto as a second species in the Orientia genus. Wild rodents were caught in Marigat, Baringo County, Kenya, and ectoparasites, including chiggers, were recovered. Rodent and chigger species were identified by taxonomic features. DNA was extracted from the chiggers and used to amplify and/or sequence the 47-kDa high temperature transmembrane protein (TSA47), the 56-kDa type-specific antigen (TSA56), and the 16S rRNA (rrs) Orientia genes. The main rodent hosts identified were Acomys wilsoni, Crocidura sp., and Mastomys natalensis, which accounted for 59.2% of the total collection. Of these, A. wilsoni and M. natalensis harbored most of the chiggers that belonged to the Neotrombicula and Microtrombicula genera. A pool of chiggers from one of M. natalensis was positive for Orientia by TSA47 PCR, but Orientia did not amplify with the TSA56 primers. On sequencing the 850 bp of the TSA47 gene, the closest phylogenetic relative was O. chuto, with 97.65% sequence homology compared to 84.63 to 84.76% for O. tsutsugamushi 16S rRNA deep sequencing also revealed O. chuto as the closest phylogenetic relative, with 99.75% sequence homology. These results and the existing immunological and molecular reports are strongly suggestive of the existence of Orientia species in Kenya. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/30282787/Identification_and_Characterization_of_Orientia_chuto_in_Trombiculid_Chigger_Mites_Collected_from_Wild_Rodents_in_Kenya_ DB - PRIME DP - Unbound Medicine ER -