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Photoelectrochemical immunoassay of lipoprotein-associated phospholipase A2 via plasmon-enhanced energy transfer between gold nanoparticles and CdS QDs/g-C3N4.
Anal Bioanal Chem. 2018 Nov; 410(29):7645-7653.AB

Abstract

A facile and feasible photoelectrochemical (PEC) immunoassay based on plasmon-enhanced energy transfer between gold nanoparticles (AuNPs) and CdS quantum dots (QDs)/g-C3N4 nanosheets was developed for the ultrasensitive detection of lipoprotein-associated phospholipase A2 (Lp-PLA2). To construct such a sensing platform, the immunosensor was prepared by immobilizing Lp-PLA2 on a CdS QDs/g-C3N4-modified electrode. A competitive-type immunoreaction was utilized for Lp-PLA2 detection, with AuNP-labeled anti-Lp-PLA2 antibody used as the competitor. Introducing AuNPs with the specific antibody for the antigen target Lp-PLA2 led to heavy quenching of the photocurrent of CdS QDs/g-C3N4 due to the plasmon-enhanced energy transfer between AuNPs and CdS QDs. The quenching efficiency decreased with increasing target Lp-PLA2 concentration. Under optimal conditions, the PEC immunosensor presented a good photocurrent response to the target Lp-PLA2 in the dynamic linear range of 0.01-300 ng mL-1, with a low detection limit of 5.3 pg mL-1. Other biomarkers and natural enzymes did not interfere with response of this system. The reproducibility and accuracy of this method for the analysis of human serum specimens were evaluated, and the results given by the method developed here were found to closely correspond to the results obtained with commercial Lp-PLA2 ELISA kits. Importantly, this protocol offers promise for the development of exciton-plasmon interaction-based PEC detection systems. Graphical abstract ᅟ.

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Authors+Show Affiliations

Department of Neurology, Chonggang General Hospital, Chongqing, 400081, China.

Department of Neurology, Chonggang General Hospital, Chongqing, 400081, China.

Department of Neurology, Chonggang General Hospital, Chongqing, 400081, China.

Department of Neurology, Chonggang General Hospital, Chongqing, 400081, China.

Department of Neurology, Chonggang General Hospital, Chongqing, 400081, China.

Department of Cardiology, Xinqiao Hospital, The Army Medical University, Chongqing, 400037, China.

Department of Cardiology, Xinqiao Hospital, The Army Medical University, Chongqing, 400037, China. zhangbocq@yeah.net.

MeSH

1-Alkyl-2-acetylglycerophosphocholine EsteraseAntibodies, MonoclonalCadmium CompoundsElectrochemical TechniquesGoldImmunoassayLightLimit of DetectionMetal NanoparticlesMicroscopy, Electron, TransmissionQuantum DotsReproducibility of ResultsSulfides

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30283999

Citation

Zhang, Dong-Ping, et al. "Photoelectrochemical Immunoassay of Lipoprotein-associated Phospholipase A2 Via Plasmon-enhanced Energy Transfer Between Gold Nanoparticles and CdS QDs/g-C3N4." Analytical and Bioanalytical Chemistry, vol. 410, no. 29, 2018, pp. 7645-7653.

Zhang DP, Wang LE, Liu XY, et al. Photoelectrochemical immunoassay of lipoprotein-associated phospholipase A2 via plasmon-enhanced energy transfer between gold nanoparticles and CdS QDs/g-C3N4. Anal Bioanal Chem. 2018;410(29):7645-7653.

Zhang, D. P., Wang, L. E., Liu, X. Y., Luo, Z. H., Zheng, L., He, Y., & Zhang, B. (2018). Photoelectrochemical immunoassay of lipoprotein-associated phospholipase A2 via plasmon-enhanced energy transfer between gold nanoparticles and CdS QDs/g-C3N4. Analytical and Bioanalytical Chemistry, 410(29), 7645-7653. https://doi.org/10.1007/s00216-018-1375-1

Zhang DP, et al. Photoelectrochemical Immunoassay of Lipoprotein-associated Phospholipase A2 Via Plasmon-enhanced Energy Transfer Between Gold Nanoparticles and CdS QDs/g-C3N4. Anal Bioanal Chem. 2018;410(29):7645-7653. PubMed PMID: 30283999.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Photoelectrochemical immunoassay of lipoprotein-associated phospholipase A2 via plasmon-enhanced energy transfer between gold nanoparticles and CdS QDs/g-C3N4. AU - Zhang,Dong-Ping, AU - Wang,Ling-E, AU - Liu,Xiao-Ying, AU - Luo,Zhi-Hua, AU - Zheng,Lei, AU - He,Yun, AU - Zhang,Bo, Y1 - 2018/10/03/ PY - 2018/05/20/received PY - 2018/09/13/accepted PY - 2018/08/07/revised PY - 2018/10/5/pubmed PY - 2019/2/9/medline PY - 2018/10/5/entrez KW - CdS quantum dots/g-C3N4 KW - Exciton–plasmon interaction KW - Gold nanoparticles KW - Lipoprotein-associated phospholipase A2 KW - Photoelectrochemical immunosensor SP - 7645 EP - 7653 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 410 IS - 29 N2 - A facile and feasible photoelectrochemical (PEC) immunoassay based on plasmon-enhanced energy transfer between gold nanoparticles (AuNPs) and CdS quantum dots (QDs)/g-C3N4 nanosheets was developed for the ultrasensitive detection of lipoprotein-associated phospholipase A2 (Lp-PLA2). To construct such a sensing platform, the immunosensor was prepared by immobilizing Lp-PLA2 on a CdS QDs/g-C3N4-modified electrode. A competitive-type immunoreaction was utilized for Lp-PLA2 detection, with AuNP-labeled anti-Lp-PLA2 antibody used as the competitor. Introducing AuNPs with the specific antibody for the antigen target Lp-PLA2 led to heavy quenching of the photocurrent of CdS QDs/g-C3N4 due to the plasmon-enhanced energy transfer between AuNPs and CdS QDs. The quenching efficiency decreased with increasing target Lp-PLA2 concentration. Under optimal conditions, the PEC immunosensor presented a good photocurrent response to the target Lp-PLA2 in the dynamic linear range of 0.01-300 ng mL-1, with a low detection limit of 5.3 pg mL-1. Other biomarkers and natural enzymes did not interfere with response of this system. The reproducibility and accuracy of this method for the analysis of human serum specimens were evaluated, and the results given by the method developed here were found to closely correspond to the results obtained with commercial Lp-PLA2 ELISA kits. Importantly, this protocol offers promise for the development of exciton-plasmon interaction-based PEC detection systems. Graphical abstract ᅟ. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/30283999/Photoelectrochemical_immunoassay_of_lipoprotein_associated_phospholipase_A2_via_plasmon_enhanced_energy_transfer_between_gold_nanoparticles_and_CdS_QDs/g_C3N4_ DB - PRIME DP - Unbound Medicine ER -