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Antimicrobial and Cytotoxicity Effects of Synthesized Silver Nanoparticles from Punica granatum Peel Extract.
Nanoscale Res Lett. 2018 Oct 04; 13(1):315.NR

Abstract

To address the growing challenges from drug-resistant microbes and tumor incidence, approaches are being undertaken to phytosynthesize metal nanoparticles, particularly silver nanoparticles, to get remedial measure. In this study, an attempt has been made to utilize a major biowaste product, pomegranate fruit peel (Punica granatum), to synthesize silver nanoparticles. The silver nanoparticles (AgNPs) were synthesized using the aqueous extract of pomegranate peel. The formation of synthesized AgNPs was confirmed through UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) as well as through the change of the colorless aqueous solution to a dark brown solution. Using UV-Vis spectroscopy, the dark brown solution showed a Plasmon resonance band peak at 378 nm in UV-Vis spectroscopy after reacting for 24, 48, and 72 h. The XRD report revealed that the AgNPs had a cubic structure. The TEM and SEM report showed the nanoparticles were equally distributed in the solution, with a spherical shape and size ranging from 20 to 40 nm and with an average particle size of 26.95 nm. EDX imaging also confirmed the presence of AgNPs. The synthesized AgNPs were found to exhibit good antimicrobial effects on Gram-negative and Gram-positive bacteria, particularly the pathogens Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27584), Proteus vulgaris (ATCC 8427), Salmonella typhi (ATCC 14028), Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (MTCC 3615), and Klebsiella pneumonia. The cytotoxic effects of AgNPs were also tested against a colon cancer cell line (RKO: ATCC® CRL-2577™), and it was observed that the viabilities were 56% and 61% on days 3 and 5, respectively, with exposure to 12.5 μg of AgNPs. This simple, economic, and eco-friendly method suggests that the AgNPs biosynthesized using pomegranate peel extract may be a novel, potent solution for the development of a drug for colon cancer that also has antibacterial activity.

Authors+Show Affiliations

Research Chair in Laser Diagnosis of Cancer, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia. Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.Research Chair in Laser Diagnosis of Cancer, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia. malsalhy@gmail.com. Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia. malsalhy@gmail.com.Stem Cell Unit, Department of Anatomy, College of Medicine King Saud, Riyadh, 11451, Kingdom of Saudi Arabia.Deparment of Biotechnology Prathyusha Engineering College, Chennai, 602025, India.Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.Research Chair in Laser Diagnosis of Cancer, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30288618

Citation

Devanesan, Sandhanasamy, et al. "Antimicrobial and Cytotoxicity Effects of Synthesized Silver Nanoparticles From Punica Granatum Peel Extract." Nanoscale Research Letters, vol. 13, no. 1, 2018, p. 315.
Devanesan S, AlSalhi MS, Balaji RV, et al. Antimicrobial and Cytotoxicity Effects of Synthesized Silver Nanoparticles from Punica granatum Peel Extract. Nanoscale Res Lett. 2018;13(1):315.
Devanesan, S., AlSalhi, M. S., Balaji, R. V., Ranjitsingh, A. J. A., Ahamed, A., Alfuraydi, A. A., AlQahtani, F. Y., Aleanizy, F. S., & Othman, A. H. (2018). Antimicrobial and Cytotoxicity Effects of Synthesized Silver Nanoparticles from Punica granatum Peel Extract. Nanoscale Research Letters, 13(1), 315. https://doi.org/10.1186/s11671-018-2731-y
Devanesan S, et al. Antimicrobial and Cytotoxicity Effects of Synthesized Silver Nanoparticles From Punica Granatum Peel Extract. Nanoscale Res Lett. 2018 Oct 4;13(1):315. PubMed PMID: 30288618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial and Cytotoxicity Effects of Synthesized Silver Nanoparticles from Punica granatum Peel Extract. AU - Devanesan,Sandhanasamy, AU - AlSalhi,Mohamad S, AU - Balaji,Radhakrishnan Vishnu, AU - Ranjitsingh,Amirtham Jacob A, AU - Ahamed,Anis, AU - Alfuraydi,Akram A, AU - AlQahtani,Fulwah Y, AU - Aleanizy,Fadilah S, AU - Othman,Ahmed H, Y1 - 2018/10/04/ PY - 2018/05/08/received PY - 2018/09/24/accepted PY - 2018/10/6/entrez PY - 2018/10/6/pubmed PY - 2018/10/6/medline KW - Antibacterial KW - Anticancer KW - Phytosynthesis KW - Pomegranate peel KW - Silver nanoparticles SP - 315 EP - 315 JF - Nanoscale research letters JO - Nanoscale Res Lett VL - 13 IS - 1 N2 - To address the growing challenges from drug-resistant microbes and tumor incidence, approaches are being undertaken to phytosynthesize metal nanoparticles, particularly silver nanoparticles, to get remedial measure. In this study, an attempt has been made to utilize a major biowaste product, pomegranate fruit peel (Punica granatum), to synthesize silver nanoparticles. The silver nanoparticles (AgNPs) were synthesized using the aqueous extract of pomegranate peel. The formation of synthesized AgNPs was confirmed through UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) as well as through the change of the colorless aqueous solution to a dark brown solution. Using UV-Vis spectroscopy, the dark brown solution showed a Plasmon resonance band peak at 378 nm in UV-Vis spectroscopy after reacting for 24, 48, and 72 h. The XRD report revealed that the AgNPs had a cubic structure. The TEM and SEM report showed the nanoparticles were equally distributed in the solution, with a spherical shape and size ranging from 20 to 40 nm and with an average particle size of 26.95 nm. EDX imaging also confirmed the presence of AgNPs. The synthesized AgNPs were found to exhibit good antimicrobial effects on Gram-negative and Gram-positive bacteria, particularly the pathogens Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27584), Proteus vulgaris (ATCC 8427), Salmonella typhi (ATCC 14028), Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (MTCC 3615), and Klebsiella pneumonia. The cytotoxic effects of AgNPs were also tested against a colon cancer cell line (RKO: ATCC® CRL-2577™), and it was observed that the viabilities were 56% and 61% on days 3 and 5, respectively, with exposure to 12.5 μg of AgNPs. This simple, economic, and eco-friendly method suggests that the AgNPs biosynthesized using pomegranate peel extract may be a novel, potent solution for the development of a drug for colon cancer that also has antibacterial activity. SN - 1931-7573 UR - https://www.unboundmedicine.com/medline/citation/30288618/Antimicrobial_and_Cytotoxicity_Effects_of_Synthesized_Silver_Nanoparticles_from_Punica_granatum_Peel_Extract_ L2 - https://dx.doi.org/10.1186/s11671-018-2731-y DB - PRIME DP - Unbound Medicine ER -
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