Tags

Type your tag names separated by a space and hit enter

A complete enzymatic capacity for long-chain polyunsaturated fatty acid biosynthesis is present in the Amazonian teleost tambaqui, Colossoma macropomum.

Abstract

In vertebrates, the essential fatty acids (FA) that satisfy the dietary requirements for a given species depend upon its desaturation and elongation capabilities to convert the C18 polyunsaturated fatty acids (PUFA), namely linoleic acid (LA, 18:2n-6) and α-linolenic acid (ALA, 18:3n-3), into the biologically active long-chain (C20-24) polyunsaturated fatty acids (LC-PUFA), including arachidonic acid (ARA, 20:4n-6), eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). Recent studies have established that tambaqui (Colossoma macropomum), an important aquaculture-produced species in Brazil, is a herbivorous fish that can fulfil its essential FA requirements with dietary provision C18 PUFA LA and ALA, although the molecular mechanisms underpinning such ability remained unclear. The present study aimed at cloning and functionally characterizing genes encoding key desaturase and elongase enzymes, namely fads2, elovl5 and elovl2, involved in the LC-PUFA biosynthetic pathways in tambaqui. First, a fads2-like desaturase was isolated from tambaqui. When expressed in yeast, the tambaqui Fads2 showed Δ6, Δ5 and Δ8 desaturase capacities within the same enzyme, enabling all desaturation reactions required for ARA, EPA and DHA biosynthesis. Moreover, tambaqui possesses two elongases that are bona fide orthologs of elovl5 and elovl2. Their functional characterization confirmed that they can operate towards a variety of PUFA substrates with chain lengths ranging from 18 to 22 carbons. Overall our results provide compelling evidence that demonstrates that all the desaturase and elongase activities required to convert LA and ALA into ARA, EPA and DHA are present in tambaqui within the three genes studied herein, i.e. fads2, elovl5 and elovl2.

Authors+Show Affiliations

CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, U. Porto - University of Porto, Terminal De Cruzeiros De Leixões. Av., General Norton De Matos S/N, 4450-208 Matosinhos, Portugal; ICBAS - Institute of Biomedical Sciences Abel Salazar, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-865, Japan.CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, U. Porto - University of Porto, Terminal De Cruzeiros De Leixões. Av., General Norton De Matos S/N, 4450-208 Matosinhos, Portugal.CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, U. Porto - University of Porto, Terminal De Cruzeiros De Leixões. Av., General Norton De Matos S/N, 4450-208 Matosinhos, Portugal.Federal University of Acre, Rodovia BR 364, Km 04, s/n - Distrito Industrial, Rio Branco, AC 69920-900, Brazil.Department of Biology, Federal University of Viçosa, Avenida Peter Henry Rolfs, s/n - Campus Universitário, Viçosa, MG 36570-900, Brazil.CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, U. Porto - University of Porto, Terminal De Cruzeiros De Leixões. Av., General Norton De Matos S/N, 4450-208 Matosinhos, Portugal.CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, U. Porto - University of Porto, Terminal De Cruzeiros De Leixões. Av., General Norton De Matos S/N, 4450-208 Matosinhos, Portugal; Department of Biology, Faculty of Sciences, University of Porto, Rua Campo Alegre s/n, 4169-007, Portugal. Electronic address: filipe.castro@ciimar.up.pt.Instituto de Acuicultura Torre de la Sal (IATS-CSIC), Ribera de Cabanes, Castellon 12595, Spain; Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK94LA, Scotland, UK. Electronic address: oscar.monroig@csic.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30290221

Citation

Ferraz, Renato B., et al. "A Complete Enzymatic Capacity for Long-chain Polyunsaturated Fatty Acid Biosynthesis Is Present in the Amazonian Teleost Tambaqui, Colossoma Macropomum." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 227, 2019, pp. 90-97.
Ferraz RB, Kabeya N, Lopes-Marques M, et al. A complete enzymatic capacity for long-chain polyunsaturated fatty acid biosynthesis is present in the Amazonian teleost tambaqui, Colossoma macropomum. Comp Biochem Physiol B, Biochem Mol Biol. 2019;227:90-97.
Ferraz, R. B., Kabeya, N., Lopes-Marques, M., Machado, A. M., Ribeiro, R. A., Salaro, A. L., ... Monroig, Ó. (2019). A complete enzymatic capacity for long-chain polyunsaturated fatty acid biosynthesis is present in the Amazonian teleost tambaqui, Colossoma macropomum. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 227, pp. 90-97. doi:10.1016/j.cbpb.2018.09.003.
Ferraz RB, et al. A Complete Enzymatic Capacity for Long-chain Polyunsaturated Fatty Acid Biosynthesis Is Present in the Amazonian Teleost Tambaqui, Colossoma Macropomum. Comp Biochem Physiol B, Biochem Mol Biol. 2019;227:90-97. PubMed PMID: 30290221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A complete enzymatic capacity for long-chain polyunsaturated fatty acid biosynthesis is present in the Amazonian teleost tambaqui, Colossoma macropomum. AU - Ferraz,Renato B, AU - Kabeya,Naoki, AU - Lopes-Marques,Mónica, AU - Machado,André M, AU - Ribeiro,Ricardo A, AU - Salaro,Ana L, AU - Ozório,Rodrigo, AU - Castro,L Filipe C, AU - Monroig,Óscar, Y1 - 2018/10/02/ PY - 2018/09/13/received PY - 2018/09/14/accepted PY - 2018/10/6/pubmed PY - 2018/12/12/medline PY - 2018/10/6/entrez KW - Biosynthesis KW - Colossoma macropomum KW - Desaturase KW - Elongase KW - Essential fatty acids SP - 90 EP - 97 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp. Biochem. Physiol. B, Biochem. Mol. Biol. VL - 227 N2 - In vertebrates, the essential fatty acids (FA) that satisfy the dietary requirements for a given species depend upon its desaturation and elongation capabilities to convert the C18 polyunsaturated fatty acids (PUFA), namely linoleic acid (LA, 18:2n-6) and α-linolenic acid (ALA, 18:3n-3), into the biologically active long-chain (C20-24) polyunsaturated fatty acids (LC-PUFA), including arachidonic acid (ARA, 20:4n-6), eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). Recent studies have established that tambaqui (Colossoma macropomum), an important aquaculture-produced species in Brazil, is a herbivorous fish that can fulfil its essential FA requirements with dietary provision C18 PUFA LA and ALA, although the molecular mechanisms underpinning such ability remained unclear. The present study aimed at cloning and functionally characterizing genes encoding key desaturase and elongase enzymes, namely fads2, elovl5 and elovl2, involved in the LC-PUFA biosynthetic pathways in tambaqui. First, a fads2-like desaturase was isolated from tambaqui. When expressed in yeast, the tambaqui Fads2 showed Δ6, Δ5 and Δ8 desaturase capacities within the same enzyme, enabling all desaturation reactions required for ARA, EPA and DHA biosynthesis. Moreover, tambaqui possesses two elongases that are bona fide orthologs of elovl5 and elovl2. Their functional characterization confirmed that they can operate towards a variety of PUFA substrates with chain lengths ranging from 18 to 22 carbons. Overall our results provide compelling evidence that demonstrates that all the desaturase and elongase activities required to convert LA and ALA into ARA, EPA and DHA are present in tambaqui within the three genes studied herein, i.e. fads2, elovl5 and elovl2. SN - 1879-1107 UR - https://www.unboundmedicine.com/medline/citation/30290221/A_complete_enzymatic_capacity_for_long_chain_polyunsaturated_fatty_acid_biosynthesis_is_present_in_the_Amazonian_teleost_tambaqui_Colossoma_macropomum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096-4959(18)30135-0 DB - PRIME DP - Unbound Medicine ER -