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Short communication: Effect of refrigerated storage on the pH and bacterial content of pasteurized human donor milk.
J Dairy Sci. 2018 Dec; 101(12):10714-10719.JD

Abstract

Once pasteurized donor milk is thawed for its administration to a preterm or sick neonate, and until it is administered, it is kept refrigerated at 4 to 6°C for 24 h. After this time, unconsumed milk is discarded. This time has not been extended, primarily because of the concern of bacterial contamination. The aim of this study was to determine the changes in pH and bacterial count when pasteurized donor milk was kept under refrigeration for a prolonged period (14 d). In this prospective study, 30 samples of pasteurized donor milk from 18 donors were analyzed. Milk was handled following the regular operating protocols established in the neonatal unit and was kept refrigerated after thawing. pH measurements and bacteriology (on blood agar and MacConkey agar plates) were performed on each sample at time 0 (immediately after thawing) and then every day for 14 d. Changes in pH of samples over time were evaluated with linear mixed-effects regression models. A slow but gradual increase in milk pH was observed starting from the first day [mean (±SD) pH of 7.30 (±0.18) at time 0 and 7.69 (±0.2) on d 14]. No bacterial growth was observed in any of the samples throughout the complete trial except in one sample, in which Bacillus flexus was isolated. In conclusion, pasteurized human donor milk maintains its microbiological quality when properly handled and refrigerated (4-6°C). The slight and continuous increase in milk pH after the first day could be due to changes in the solubility of calcium and phosphate during refrigerated storage.

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Authors+Show Affiliations

Vázquez-Román S
Neonatology Department, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain; Pediatrics Department, Universidad Complutense, 28040 Madrid, Spain. Electronic address: sara.vazquezroman@gmail.com.
Escuder-Vieco D
Neonatology Department, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain; Pediatrics Department, Universidad Complutense, 28040 Madrid, Spain.
Martín-Pelegrina MD
Neonatology Department, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain; Pediatrics Department, Universidad Complutense, 28040 Madrid, Spain.
Muñoz-Amat B
Neonatology Department, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain; Pediatrics Department, Universidad Complutense, 28040 Madrid, Spain.
Fernández-Álvarez L
Pediatrics Department, Universidad Complutense, 28040 Madrid, Spain; Nutrition and Bromatology Department, Universidad Complutense, 28040 Madrid, Spain.
Brañas-García P
Microbiology Department, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain.
Lora-Pablos D
Clinical Research Unit, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain; CIBER de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.
Beceiro-Mosquera J
Neonatology Department, Hospital Universitario Príncipe de Asturias, 28805 Alcalá de Henares, Spain.
Pallás-Alonso CR
Neonatology Department, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain; Pediatrics Department, Universidad Complutense, 28040 Madrid, Spain.

MeSH

BacteriaBacterial LoadFood MicrobiologyFood PreservationHumansHydrogen-Ion ConcentrationMilk, HumanPasteurizationProspective StudiesRefrigerationTime FactorsTissue Donors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30292544

Citation

Vázquez-Román, S, et al. "Short Communication: Effect of Refrigerated Storage On the pH and Bacterial Content of Pasteurized Human Donor Milk." Journal of Dairy Science, vol. 101, no. 12, 2018, pp. 10714-10719.
Vázquez-Román S, Escuder-Vieco D, Martín-Pelegrina MD, et al. Short communication: Effect of refrigerated storage on the pH and bacterial content of pasteurized human donor milk. J Dairy Sci. 2018;101(12):10714-10719.
Vázquez-Román, S., Escuder-Vieco, D., Martín-Pelegrina, M. D., Muñoz-Amat, B., Fernández-Álvarez, L., Brañas-García, P., Lora-Pablos, D., Beceiro-Mosquera, J., & Pallás-Alonso, C. R. (2018). Short communication: Effect of refrigerated storage on the pH and bacterial content of pasteurized human donor milk. Journal of Dairy Science, 101(12), 10714-10719. https://doi.org/10.3168/jds.2018-14984
Vázquez-Román S, et al. Short Communication: Effect of Refrigerated Storage On the pH and Bacterial Content of Pasteurized Human Donor Milk. J Dairy Sci. 2018;101(12):10714-10719. PubMed PMID: 30292544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Short communication: Effect of refrigerated storage on the pH and bacterial content of pasteurized human donor milk. AU - Vázquez-Román,S, AU - Escuder-Vieco,D, AU - Martín-Pelegrina,M D, AU - Muñoz-Amat,B, AU - Fernández-Álvarez,L, AU - Brañas-García,P, AU - Lora-Pablos,D, AU - Beceiro-Mosquera,J, AU - Pallás-Alonso,C R, Y1 - 2018/10/03/ PY - 2018/04/27/received PY - 2018/07/24/accepted PY - 2018/10/8/pubmed PY - 2019/1/23/medline PY - 2018/10/8/entrez KW - acidity KW - pasteurized donor milk KW - refrigerated KW - storage SP - 10714 EP - 10719 JF - Journal of dairy science JO - J Dairy Sci VL - 101 IS - 12 N2 - Once pasteurized donor milk is thawed for its administration to a preterm or sick neonate, and until it is administered, it is kept refrigerated at 4 to 6°C for 24 h. After this time, unconsumed milk is discarded. This time has not been extended, primarily because of the concern of bacterial contamination. The aim of this study was to determine the changes in pH and bacterial count when pasteurized donor milk was kept under refrigeration for a prolonged period (14 d). In this prospective study, 30 samples of pasteurized donor milk from 18 donors were analyzed. Milk was handled following the regular operating protocols established in the neonatal unit and was kept refrigerated after thawing. pH measurements and bacteriology (on blood agar and MacConkey agar plates) were performed on each sample at time 0 (immediately after thawing) and then every day for 14 d. Changes in pH of samples over time were evaluated with linear mixed-effects regression models. A slow but gradual increase in milk pH was observed starting from the first day [mean (±SD) pH of 7.30 (±0.18) at time 0 and 7.69 (±0.2) on d 14]. No bacterial growth was observed in any of the samples throughout the complete trial except in one sample, in which Bacillus flexus was isolated. In conclusion, pasteurized human donor milk maintains its microbiological quality when properly handled and refrigerated (4-6°C). The slight and continuous increase in milk pH after the first day could be due to changes in the solubility of calcium and phosphate during refrigerated storage. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/30292544/Short_communication:_Effect_of_refrigerated_storage_on_the_pH_and_bacterial_content_of_pasteurized_human_donor_milk_ DB - PRIME DP - Unbound Medicine ER -