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Effect of nanosecond pulsed electric field treatment on cell proliferation of microalgae.
Bioresour Technol. 2019 Jan; 271:402-408.BT

Abstract

Photoautotrophic microalgae based biorefinery concepts are currently not competitive compared to other established production systems. Therefore, innovative upstream processes need to be developed to increase the competitiveness of photoautotrophic microalgae biorefinery concepts. Abiotic sub-lethal stress induction via nanosecond pulsed electric field (nsPEF) treatment might be a viable process to increase the efficiency of photoautotrophic microalgae cultivation. In this work, an increased cell growth after nsPEF treatment was observable. Application of nsPEF to highly proliferating cells in a repetitive process resulted in a statistical significant increase in cell growth (p = 0.009). The effect was most pronounced after five days wherefore cellular structures and processes were analyzed to reveal a possible mechanism. Within this work, a protocol for increased cell proliferation with a possible mechanism was derived, which improves competitiveness of photoautotrophic microalgae biorefineries in the future. However, based on the derived mechanism, the results are also relevant for other microorganisms.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Buchmann L
ETH Zurich, Department of Health Sciences and Technology, Institute of Food, Nutrition and Health, IFNH, Sustainable Food Processing Laboratory, Schmelzbergstrasse 9, Zurich 8092, Switzerland. Electronic address: Leandro.Buchmann@hest.ethz.ch.
Frey W
Karlsruhe Institute of Technology, KIT, Institute for Pulsed Power and Microwave Technology, IHM, Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen 76344, Germany. Electronic address: Wolfgang.Frey@kit.edu.
Gusbeth C
Karlsruhe Institute of Technology, KIT, Institute for Pulsed Power and Microwave Technology, IHM, Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen 76344, Germany. Electronic address: Christian.Gusbeth@kit.edu.
Ravaynia PS
ETH Zurich, Department of Biosystems Science and Engineering, Bio Engineering Laboratory, Mattenstrasse 26, Basel 4058, Switzerland. Electronic address: Paolo.Ravaynia@bsse.ethz.ch.
Mathys A
ETH Zurich, Department of Health Sciences and Technology, Institute of Food, Nutrition and Health, IFNH, Sustainable Food Processing Laboratory, Schmelzbergstrasse 9, Zurich 8092, Switzerland. Electronic address: Alexander.Mathys@hest.ethz.ch.

MeSH

Cell ProliferationCyanobacteriaElectricityMicroalgae

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30296747

Citation

Buchmann, Leandro, et al. "Effect of Nanosecond Pulsed Electric Field Treatment On Cell Proliferation of Microalgae." Bioresource Technology, vol. 271, 2019, pp. 402-408.
Buchmann L, Frey W, Gusbeth C, et al. Effect of nanosecond pulsed electric field treatment on cell proliferation of microalgae. Bioresour Technol. 2019;271:402-408.
Buchmann, L., Frey, W., Gusbeth, C., Ravaynia, P. S., & Mathys, A. (2019). Effect of nanosecond pulsed electric field treatment on cell proliferation of microalgae. Bioresource Technology, 271, 402-408. https://doi.org/10.1016/j.biortech.2018.09.124
Buchmann L, et al. Effect of Nanosecond Pulsed Electric Field Treatment On Cell Proliferation of Microalgae. Bioresour Technol. 2019;271:402-408. PubMed PMID: 30296747.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of nanosecond pulsed electric field treatment on cell proliferation of microalgae. AU - Buchmann,Leandro, AU - Frey,Wolfgang, AU - Gusbeth,Christian, AU - Ravaynia,Paolo S, AU - Mathys,Alexander, Y1 - 2018/09/26/ PY - 2018/08/31/received PY - 2018/09/23/revised PY - 2018/09/24/accepted PY - 2018/10/9/pubmed PY - 2019/4/12/medline PY - 2018/10/9/entrez KW - Abiotic stress KW - Arthrospira platensis KW - Cell proliferation KW - Microalgae cultivation KW - Nanosecond pulsed electric field SP - 402 EP - 408 JF - Bioresource technology JO - Bioresour Technol VL - 271 N2 - Photoautotrophic microalgae based biorefinery concepts are currently not competitive compared to other established production systems. Therefore, innovative upstream processes need to be developed to increase the competitiveness of photoautotrophic microalgae biorefinery concepts. Abiotic sub-lethal stress induction via nanosecond pulsed electric field (nsPEF) treatment might be a viable process to increase the efficiency of photoautotrophic microalgae cultivation. In this work, an increased cell growth after nsPEF treatment was observable. Application of nsPEF to highly proliferating cells in a repetitive process resulted in a statistical significant increase in cell growth (p = 0.009). The effect was most pronounced after five days wherefore cellular structures and processes were analyzed to reveal a possible mechanism. Within this work, a protocol for increased cell proliferation with a possible mechanism was derived, which improves competitiveness of photoautotrophic microalgae biorefineries in the future. However, based on the derived mechanism, the results are also relevant for other microorganisms. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/30296747/Effect_of_nanosecond_pulsed_electric_field_treatment_on_cell_proliferation_of_microalgae_ DB - PRIME DP - Unbound Medicine ER -