Tags

Type your tag names separated by a space and hit enter

Composition Analysis by UPLC-PDA-ESI (-)-HRMS and Antioxidant Activity Using Saccharomyces cerevisiae Model of Herbal Teas and Green Teas from Hainan.
Molecules. 2018 Oct 06; 23(10)M

Abstract

Different teas from everywhere are very useful and have been extensively studied. We studied the antioxidant activity of herbal teas and green teas from Hainan, Mallotus oblongifolius Muell. Arg. (MO), Ilex kudingcha C.J. Tseng (KD), Camellia sinensis var. assamica (J. W. Mast.) Kitam. Hainan Dayezhong (DY), and Camellia sinensis (L.) O. Ktze. (produced from Hainan Baisha (BS)). The total phenol content and total flavonoid content from water extracts, resin extracts and fractions of herbal teas and green teas were compared. Later, eight fractions of herbal teas and green teas were subjected to UPLC-PDA-ESI-(-)-HRMS. We determined 1-diphenyl -2-picryl-hydrazyl radical and hydroxyl free radical scavenging activity by electron paramagnetic resonance spectroscopy. We subjected Saccharomyces cerevisiae to hydrogen peroxide, stress and evaluated antioxidant activity of herbal teas and green teas in cellulo. The experiment identified more than 14 potential antioxidant compounds from herbal teas and green teas. The herbal teas and green teas had a clearance rate higher than ferulic acid at the same concentrations. MO best reduced intracellular oxidation levels and increased catalase, glutathione reductase activities, glutathione reduced and glutathione oxidized content. KD had the highest cell survival rate and reduced cell lipid peroxidation. DY best improved superoxide dismutase activity and BS was the most active in the halo test. Therefore, we concluded that MO had stronger antioxidant activity than other herbal teas and green teas from Hainan, especially, which reduce S. cerevisiae oxidative stress under H₂O₂ stress.

Authors+Show Affiliations

Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan, 570228, China. hnulihua@163.com.Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan, 570228, China. daivemuwly@126.com.Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan, 570228, China. yanpluo2012@hainu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30301226

Citation

Li, Hua, et al. "Composition Analysis By UPLC-PDA-ESI (-)-HRMS and Antioxidant Activity Using Saccharomyces Cerevisiae Model of Herbal Teas and Green Teas From Hainan." Molecules (Basel, Switzerland), vol. 23, no. 10, 2018.
Li H, Wang L, Luo Y. Composition Analysis by UPLC-PDA-ESI (-)-HRMS and Antioxidant Activity Using Saccharomyces cerevisiae Model of Herbal Teas and Green Teas from Hainan. Molecules. 2018;23(10).
Li, H., Wang, L., & Luo, Y. (2018). Composition Analysis by UPLC-PDA-ESI (-)-HRMS and Antioxidant Activity Using Saccharomyces cerevisiae Model of Herbal Teas and Green Teas from Hainan. Molecules (Basel, Switzerland), 23(10). https://doi.org/10.3390/molecules23102550
Li H, Wang L, Luo Y. Composition Analysis By UPLC-PDA-ESI (-)-HRMS and Antioxidant Activity Using Saccharomyces Cerevisiae Model of Herbal Teas and Green Teas From Hainan. Molecules. 2018 Oct 6;23(10) PubMed PMID: 30301226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Composition Analysis by UPLC-PDA-ESI (-)-HRMS and Antioxidant Activity Using Saccharomyces cerevisiae Model of Herbal Teas and Green Teas from Hainan. AU - Li,Hua, AU - Wang,Lanying, AU - Luo,Yanping, Y1 - 2018/10/06/ PY - 2018/08/28/received PY - 2018/09/30/revised PY - 2018/10/03/accepted PY - 2018/10/11/entrez PY - 2018/10/12/pubmed PY - 2018/12/13/medline KW - EPR spectroscopy KW - H2O2 stress KW - Saccharomyces cerevisiae KW - UPLC-PDA-ESI-(−)-HRMS KW - antioxidant activity JF - Molecules (Basel, Switzerland) JO - Molecules VL - 23 IS - 10 N2 - Different teas from everywhere are very useful and have been extensively studied. We studied the antioxidant activity of herbal teas and green teas from Hainan, Mallotus oblongifolius Muell. Arg. (MO), Ilex kudingcha C.J. Tseng (KD), Camellia sinensis var. assamica (J. W. Mast.) Kitam. Hainan Dayezhong (DY), and Camellia sinensis (L.) O. Ktze. (produced from Hainan Baisha (BS)). The total phenol content and total flavonoid content from water extracts, resin extracts and fractions of herbal teas and green teas were compared. Later, eight fractions of herbal teas and green teas were subjected to UPLC-PDA-ESI-(-)-HRMS. We determined 1-diphenyl -2-picryl-hydrazyl radical and hydroxyl free radical scavenging activity by electron paramagnetic resonance spectroscopy. We subjected Saccharomyces cerevisiae to hydrogen peroxide, stress and evaluated antioxidant activity of herbal teas and green teas in cellulo. The experiment identified more than 14 potential antioxidant compounds from herbal teas and green teas. The herbal teas and green teas had a clearance rate higher than ferulic acid at the same concentrations. MO best reduced intracellular oxidation levels and increased catalase, glutathione reductase activities, glutathione reduced and glutathione oxidized content. KD had the highest cell survival rate and reduced cell lipid peroxidation. DY best improved superoxide dismutase activity and BS was the most active in the halo test. Therefore, we concluded that MO had stronger antioxidant activity than other herbal teas and green teas from Hainan, especially, which reduce S. cerevisiae oxidative stress under H₂O₂ stress. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30301226/Composition_Analysis_by_UPLC_PDA_ESI_____HRMS_and_Antioxidant_Activity_Using_Saccharomyces_cerevisiae_Model_of_Herbal_Teas_and_Green_Teas_from_Hainan_ L2 - https://www.mdpi.com/resolver?pii=molecules23102550 DB - PRIME DP - Unbound Medicine ER -