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Fluoroquinolone Resistance Mechanisms in Shigella Isolates in Shanghai, China, Between 2010 and 2015.
Microb Drug Resist. 2019 Mar; 25(2):212-218.MD

Abstract

OBJECTIVES

This study aimed to investigate the antimicrobial susceptibility of Shigella isolated in Shanghai, China and to determine the genetic basis of its resistance to fluoroquinolones.

MATERIALS AND METHODS

A total of 402 strains of Shigella, including 145 Shigella flexneri and 257 Shigella sonnei isolates, were analyzed. The Kirby-Bauer disk diffusion method was used to determine the susceptibility of the strains to 13 antimicrobials. Minimum inhibitory concentration of ciprofloxacin was determined by E-test. Mutations within the quinolone resistance-determining regions (QRDRs) of gyrA and parC and in the plasmid-mediated quinolone resistance (PMQR) genes, including qnrA, qnrB, qnrS, and aac (6')-Ib-cr, were detected by polymerase chain reaction. All the products were then sequenced.

RESULTS

Most of the Shigella isolates were found to be resistant to nalidixic acid (96.4%), streptomycin (96.4%), ampicillin (86.2%), tetracycline (79.8%), and sulfamethoxazole/trimethoprim (80.6%). S. flexneri isolates showed a significantly higher resistance to cefepime (33.6%), ciprofloxacin (54.2%), norfloxacin (34.1%), and levofloxacin (12.1%) compared with that observed for the S. sonnei strains (χ2 analysis, p < 0.05). Three mutations (Ser83, Asp87, and His211) in gyrA and one mutation (Ser80) in parC were detected. Of 257 S. sonnei isolates, 11.7% possessed gyrA mutations and 2% had parC mutations. Of 145 S. flexneri isolates, 98.6% possessed gyrA mutations and 97.9% had parC mutations. The plasmid-mediated resistance genes of qnrS and aac (6')-Ib-cr were detected among 17 strains (4.2%).

CONCLUSIONS

The mutation percentage within the QRDR of S. flexneri was as high as 98.6 in gyrA and 97.9 in parC. The significant abundance of mutations within QRDRs conferred high levels of fluoroquinolone resistance. Moreover, the PMQR genes, particularly qnrS, played an important role in the decreased susceptibility of Shigella to fluoroquinolones.

Authors+Show Affiliations

1 Department of Clinical Laboratory, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine , Shanghai, China .2 Laboratory of Bacterial, Shanghai Center for Disease Control and Prevention , Shanghai, China .2 Laboratory of Bacterial, Shanghai Center for Disease Control and Prevention , Shanghai, China .2 Laboratory of Bacterial, Shanghai Center for Disease Control and Prevention , Shanghai, China .2 Laboratory of Bacterial, Shanghai Center for Disease Control and Prevention , Shanghai, China .1 Department of Clinical Laboratory, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine , Shanghai, China .

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30307807

Citation

Zhang, Wen-Xia, et al. "Fluoroquinolone Resistance Mechanisms in Shigella Isolates in Shanghai, China, Between 2010 and 2015." Microbial Drug Resistance (Larchmont, N.Y.), vol. 25, no. 2, 2019, pp. 212-218.
Zhang WX, Chen HY, Tu LH, et al. Fluoroquinolone Resistance Mechanisms in Shigella Isolates in Shanghai, China, Between 2010 and 2015. Microb Drug Resist. 2019;25(2):212-218.
Zhang, W. X., Chen, H. Y., Tu, L. H., Xi, M. F., Chen, M., & Zhang, J. (2019). Fluoroquinolone Resistance Mechanisms in Shigella Isolates in Shanghai, China, Between 2010 and 2015. Microbial Drug Resistance (Larchmont, N.Y.), 25(2), 212-218. https://doi.org/10.1089/mdr.2018.0113
Zhang WX, et al. Fluoroquinolone Resistance Mechanisms in Shigella Isolates in Shanghai, China, Between 2010 and 2015. Microb Drug Resist. 2019;25(2):212-218. PubMed PMID: 30307807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluoroquinolone Resistance Mechanisms in Shigella Isolates in Shanghai, China, Between 2010 and 2015. AU - Zhang,Wen-Xia, AU - Chen,Hong-You, AU - Tu,Li-Hong, AU - Xi,Man-Fang, AU - Chen,Min, AU - Zhang,Jue, Y1 - 2018/10/10/ PY - 2018/10/12/pubmed PY - 2019/7/6/medline PY - 2018/10/12/entrez KW - KW - DNA gyrase KW - antimicrobial resistance KW - quinolone KW - topoisomerase IV SP - 212 EP - 218 JF - Microbial drug resistance (Larchmont, N.Y.) JO - Microb Drug Resist VL - 25 IS - 2 N2 - OBJECTIVES: This study aimed to investigate the antimicrobial susceptibility of Shigella isolated in Shanghai, China and to determine the genetic basis of its resistance to fluoroquinolones. MATERIALS AND METHODS: A total of 402 strains of Shigella, including 145 Shigella flexneri and 257 Shigella sonnei isolates, were analyzed. The Kirby-Bauer disk diffusion method was used to determine the susceptibility of the strains to 13 antimicrobials. Minimum inhibitory concentration of ciprofloxacin was determined by E-test. Mutations within the quinolone resistance-determining regions (QRDRs) of gyrA and parC and in the plasmid-mediated quinolone resistance (PMQR) genes, including qnrA, qnrB, qnrS, and aac (6')-Ib-cr, were detected by polymerase chain reaction. All the products were then sequenced. RESULTS: Most of the Shigella isolates were found to be resistant to nalidixic acid (96.4%), streptomycin (96.4%), ampicillin (86.2%), tetracycline (79.8%), and sulfamethoxazole/trimethoprim (80.6%). S. flexneri isolates showed a significantly higher resistance to cefepime (33.6%), ciprofloxacin (54.2%), norfloxacin (34.1%), and levofloxacin (12.1%) compared with that observed for the S. sonnei strains (χ2 analysis, p < 0.05). Three mutations (Ser83, Asp87, and His211) in gyrA and one mutation (Ser80) in parC were detected. Of 257 S. sonnei isolates, 11.7% possessed gyrA mutations and 2% had parC mutations. Of 145 S. flexneri isolates, 98.6% possessed gyrA mutations and 97.9% had parC mutations. The plasmid-mediated resistance genes of qnrS and aac (6')-Ib-cr were detected among 17 strains (4.2%). CONCLUSIONS: The mutation percentage within the QRDR of S. flexneri was as high as 98.6 in gyrA and 97.9 in parC. The significant abundance of mutations within QRDRs conferred high levels of fluoroquinolone resistance. Moreover, the PMQR genes, particularly qnrS, played an important role in the decreased susceptibility of Shigella to fluoroquinolones. SN - 1931-8448 UR - https://www.unboundmedicine.com/medline/citation/30307807/Fluoroquinolone_Resistance_Mechanisms_in_Shigella_Isolates_in_Shanghai_China_Between_2010_and_2015_ L2 - https://www.liebertpub.com/doi/10.1089/mdr.2018.0113?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -