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In Vitro Antibacterial Activity of Propyl-Propane-Thiosulfinate and Propyl-Propane-Thiosulfonate Derived from Allium spp. against Gram-Negative and Gram-Positive Multidrug-Resistant Bacteria Isolated from Human Samples.
Biomed Res Int. 2018; 2018:7861207.BR

Abstract

Background

The aim of this study was to compare the in vitro antibacterial activity of two compounds derived from Alliaceae, PTS (propyl-propane-thiosulfinate), and PTSO (propyl-propane-thiosulfonate), with that of other antibiotics commonly used against bacteria isolated from humans.

Materials and Methods

A total of 212 gram-negative bacilli and 267 gram-positive cocci isolated from human clinical samples and resistant to at least one group of antibiotics were selected. In order to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) to various antibiotics as well as PTS and PTSO, all isolates underwent broth microdilution assay.

Results

PTS showed moderate activity against Enterobacteriaceae with MIC50 (and MBC50) and MIC90 (and MBC90) values of 256-512 mg/L, while PTSO showed greater activity with MIC50 and MIC90 values of 64-128 mg/L and MBC50 and MBC90 values of 128-512 mg/L. These data show the bactericidal activity of both compounds and indicate that PTSO was more active than PTS against this group of bacteria. Both compounds showed lower activity against P. aeruginosa (MIC50 = 1024 mg/L, MIC90 = 2048 mg/L, MBC50 = 2048 mg/L, and MBC90 = 2048 mg/L, for PTS; MIC50 = 512 mg/L, MIC90 = 1024 mg/L, MBC50 = 512 mg/L, and MBC90 = 2048 mg/L, for PTSO) compared to those obtained in others nonfermenting gram-negative bacilli (MIC50 = 128 mg/L, MIC90 = 512 mg/L, MBC50 = 128 mg/L, and MBC90 = 512 mg/L, for PTS; MIC50 = 64 mg/L, MIC90 = 256 mg/L, MBC50 = 64 mg/L, and MBC90 = 256 mg/L, for PTSO) and also indicate the bactericidal activity of both compounds against these groups of bacteria. Finally, the activity against S. aureus, E. faecalis, and S. agalactiae was higher than that observed against enterobacteria, especially in the case of PTSO (MIC50 = 8 mg/L, MIC90 = 8 mg/L, MBC50 = 32 mg/L, and MBC90 = 64 mg/L, in S. aureus; MIC50 = 4 mg/L, MIC90 = 8 mg/L, MBC50 = 8 mg/L, and MBC90 = 16 mg/L, in E. faecalis and S. agalactiae).

Conclusion

PTS and PTSO have a significant broad spectrum antibacterial activity against multiresistant bacteria isolated from human clinical samples. Preliminary results in present work provide basic and useful information for development and potential use of these compounds in the treatment of human infections.

Authors+Show Affiliations

Department of Microbiology, School of Medicine and PhD Program in Clinical Medicine and Public Health, University of Granada-ibs, Granada, Spain.Department of Microbiology, School of Medicine and PhD Program in Clinical Medicine and Public Health, University of Granada-ibs, Granada, Spain.Department of Microbiology, School of Medicine and PhD Program in Clinical Medicine and Public Health, University of Granada-ibs, Granada, Spain.Department of Microbiology and Biotechnology, DMC Research Center, Granada, Spain.Department of Microbiology and Biotechnology, DMC Research Center, Granada, Spain.Unit of Methodology of Research and Biostatistics, Virgen de las Nieves University Hospital-ibs, Granada, Spain.Department of Microbiology, School of Medicine and PhD Program in Clinical Medicine and Public Health, University of Granada-ibs, Granada, Spain. Laboratory of Microbiology, Virgen de las Nieves University Hospital-ibs, Granada, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30310819

Citation

Sorlozano-Puerto, Antonio, et al. "In Vitro Antibacterial Activity of Propyl-Propane-Thiosulfinate and Propyl-Propane-Thiosulfonate Derived From Allium Spp. Against Gram-Negative and Gram-Positive Multidrug-Resistant Bacteria Isolated From Human Samples." BioMed Research International, vol. 2018, 2018, p. 7861207.
Sorlozano-Puerto A, Albertuz-Crespo M, Lopez-Machado I, et al. In Vitro Antibacterial Activity of Propyl-Propane-Thiosulfinate and Propyl-Propane-Thiosulfonate Derived from Allium spp. against Gram-Negative and Gram-Positive Multidrug-Resistant Bacteria Isolated from Human Samples. Biomed Res Int. 2018;2018:7861207.
Sorlozano-Puerto, A., Albertuz-Crespo, M., Lopez-Machado, I., Ariza-Romero, J. J., Baños-Arjona, A., Exposito-Ruiz, M., & Gutierrez-Fernandez, J. (2018). In Vitro Antibacterial Activity of Propyl-Propane-Thiosulfinate and Propyl-Propane-Thiosulfonate Derived from Allium spp. against Gram-Negative and Gram-Positive Multidrug-Resistant Bacteria Isolated from Human Samples. BioMed Research International, 2018, 7861207. https://doi.org/10.1155/2018/7861207
Sorlozano-Puerto A, et al. In Vitro Antibacterial Activity of Propyl-Propane-Thiosulfinate and Propyl-Propane-Thiosulfonate Derived From Allium Spp. Against Gram-Negative and Gram-Positive Multidrug-Resistant Bacteria Isolated From Human Samples. Biomed Res Int. 2018;2018:7861207. PubMed PMID: 30310819.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In Vitro Antibacterial Activity of Propyl-Propane-Thiosulfinate and Propyl-Propane-Thiosulfonate Derived from Allium spp. against Gram-Negative and Gram-Positive Multidrug-Resistant Bacteria Isolated from Human Samples. AU - Sorlozano-Puerto,Antonio, AU - Albertuz-Crespo,Maria, AU - Lopez-Machado,Isaac, AU - Ariza-Romero,Juan Jose, AU - Baños-Arjona,Alberto, AU - Exposito-Ruiz,Manuela, AU - Gutierrez-Fernandez,Jose, Y1 - 2018/09/17/ PY - 2018/04/30/received PY - 2018/08/01/accepted PY - 2018/10/13/entrez PY - 2018/10/13/pubmed PY - 2019/1/8/medline SP - 7861207 EP - 7861207 JF - BioMed research international JO - Biomed Res Int VL - 2018 N2 - Background: The aim of this study was to compare the in vitro antibacterial activity of two compounds derived from Alliaceae, PTS (propyl-propane-thiosulfinate), and PTSO (propyl-propane-thiosulfonate), with that of other antibiotics commonly used against bacteria isolated from humans. Materials and Methods: A total of 212 gram-negative bacilli and 267 gram-positive cocci isolated from human clinical samples and resistant to at least one group of antibiotics were selected. In order to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) to various antibiotics as well as PTS and PTSO, all isolates underwent broth microdilution assay. Results: PTS showed moderate activity against Enterobacteriaceae with MIC50 (and MBC50) and MIC90 (and MBC90) values of 256-512 mg/L, while PTSO showed greater activity with MIC50 and MIC90 values of 64-128 mg/L and MBC50 and MBC90 values of 128-512 mg/L. These data show the bactericidal activity of both compounds and indicate that PTSO was more active than PTS against this group of bacteria. Both compounds showed lower activity against P. aeruginosa (MIC50 = 1024 mg/L, MIC90 = 2048 mg/L, MBC50 = 2048 mg/L, and MBC90 = 2048 mg/L, for PTS; MIC50 = 512 mg/L, MIC90 = 1024 mg/L, MBC50 = 512 mg/L, and MBC90 = 2048 mg/L, for PTSO) compared to those obtained in others nonfermenting gram-negative bacilli (MIC50 = 128 mg/L, MIC90 = 512 mg/L, MBC50 = 128 mg/L, and MBC90 = 512 mg/L, for PTS; MIC50 = 64 mg/L, MIC90 = 256 mg/L, MBC50 = 64 mg/L, and MBC90 = 256 mg/L, for PTSO) and also indicate the bactericidal activity of both compounds against these groups of bacteria. Finally, the activity against S. aureus, E. faecalis, and S. agalactiae was higher than that observed against enterobacteria, especially in the case of PTSO (MIC50 = 8 mg/L, MIC90 = 8 mg/L, MBC50 = 32 mg/L, and MBC90 = 64 mg/L, in S. aureus; MIC50 = 4 mg/L, MIC90 = 8 mg/L, MBC50 = 8 mg/L, and MBC90 = 16 mg/L, in E. faecalis and S. agalactiae). Conclusion: PTS and PTSO have a significant broad spectrum antibacterial activity against multiresistant bacteria isolated from human clinical samples. Preliminary results in present work provide basic and useful information for development and potential use of these compounds in the treatment of human infections. SN - 2314-6141 UR - https://www.unboundmedicine.com/medline/citation/30310819/In_Vitro_Antibacterial_Activity_of_Propyl_Propane_Thiosulfinate_and_Propyl_Propane_Thiosulfonate_Derived_from_Allium_spp__against_Gram_Negative_and_Gram_Positive_Multidrug_Resistant_Bacteria_Isolated_from_Human_Samples_ L2 - https://doi.org/10.1155/2018/7861207 DB - PRIME DP - Unbound Medicine ER -