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Intracerebral infection of Cebus apella with the XJ-Clone 3 strain of Junín virus.
J Med Virol. 1987 Mar; 21(3):257-68.JM

Abstract

To assess the usefulness of the South American primate Cebus apella as a model for neurovirulence of Junín virus, eight monkeys were inoculated with 10(5) LD50 of the attenuated XJ-Clone 3 Junín virus strain by the intrathalamic route. After the second week, weight loss and polyadenopathies were observed in most animals, one-half of which had a transient leukothrombocytopenia. Moderate clinical central nervous system (CNS) involvement was present in four of eight monkeys, while the rest had only mild neurologic signs. All recovered except one, which developed a deep coma and was killed in a pre-mortem stage at 18 days post-infection (pi). Junín virus was isolated from the throat from five, from the blood from three, and from the brain from two monkeys. In the most severely ill animal, virus titers higher than viremia were detected in both inoculated and contralateral brain hemispheres, as well as in lung, lymph node, and small intestine. Junín antigens and "in vivo" bound immunoglobulins were detected by immunofluorescence (IF) in the brain of four animals at 18, 21, 40, and 155 days pi. Moderate lymphocytic parenchymal and meningeal infiltration were observed in the brain of four animals, and gliosis was also present in the most affected monkey. Although the clinical response to infection was not uniform, all infected monkeys developed high IF antibodies. Cebus apella cannot be used as a highly sensitive model for Argentine hemorrhagic fever (AHF). However, the results obtained show that the XJ-Clone 3 strain can replicate in the primate CNS and to induce lesions and immunoglobulin deposition. In addition, viral persistence is suggested by the late detection of viral antigens in brain at 40 and 155 days pi.

Authors

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Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3031201

Citation

Carballal, G, et al. "Intracerebral Infection of Cebus Apella With the XJ-Clone 3 Strain of Junín Virus." Journal of Medical Virology, vol. 21, no. 3, 1987, pp. 257-68.
Carballal G, Oubiña JR, Molinas FC, et al. Intracerebral infection of Cebus apella with the XJ-Clone 3 strain of Junín virus. J Med Virol. 1987;21(3):257-68.
Carballal, G., Oubiña, J. R., Molinas, F. C., Nagle, C., de la Vega, M. T., Videla, C., & Elsner, B. (1987). Intracerebral infection of Cebus apella with the XJ-Clone 3 strain of Junín virus. Journal of Medical Virology, 21(3), 257-68.
Carballal G, et al. Intracerebral Infection of Cebus Apella With the XJ-Clone 3 Strain of Junín Virus. J Med Virol. 1987;21(3):257-68. PubMed PMID: 3031201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intracerebral infection of Cebus apella with the XJ-Clone 3 strain of Junín virus. AU - Carballal,G, AU - Oubiña,J R, AU - Molinas,F C, AU - Nagle,C, AU - de la Vega,M T, AU - Videla,C, AU - Elsner,B, PY - 1987/3/1/pubmed PY - 1987/3/1/medline PY - 1987/3/1/entrez SP - 257 EP - 68 JF - Journal of medical virology JO - J Med Virol VL - 21 IS - 3 N2 - To assess the usefulness of the South American primate Cebus apella as a model for neurovirulence of Junín virus, eight monkeys were inoculated with 10(5) LD50 of the attenuated XJ-Clone 3 Junín virus strain by the intrathalamic route. After the second week, weight loss and polyadenopathies were observed in most animals, one-half of which had a transient leukothrombocytopenia. Moderate clinical central nervous system (CNS) involvement was present in four of eight monkeys, while the rest had only mild neurologic signs. All recovered except one, which developed a deep coma and was killed in a pre-mortem stage at 18 days post-infection (pi). Junín virus was isolated from the throat from five, from the blood from three, and from the brain from two monkeys. In the most severely ill animal, virus titers higher than viremia were detected in both inoculated and contralateral brain hemispheres, as well as in lung, lymph node, and small intestine. Junín antigens and "in vivo" bound immunoglobulins were detected by immunofluorescence (IF) in the brain of four animals at 18, 21, 40, and 155 days pi. Moderate lymphocytic parenchymal and meningeal infiltration were observed in the brain of four animals, and gliosis was also present in the most affected monkey. Although the clinical response to infection was not uniform, all infected monkeys developed high IF antibodies. Cebus apella cannot be used as a highly sensitive model for Argentine hemorrhagic fever (AHF). However, the results obtained show that the XJ-Clone 3 strain can replicate in the primate CNS and to induce lesions and immunoglobulin deposition. In addition, viral persistence is suggested by the late detection of viral antigens in brain at 40 and 155 days pi. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/3031201/Intracerebral_infection_of_Cebus_apella_with_the_XJ_Clone_3_strain_of_Junín_virus_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0146-6615&date=1987&volume=21&issue=3&spage=257 DB - PRIME DP - Unbound Medicine ER -