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Target separation of flavonoids from Saxifraga tangutica using two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography.
J Sep Sci 2018; 41(24):4419-4429JS

Abstract

An orthogonally (80.3%) preparative two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography method has been established for the isolation and purification of flavonoids from Saxifraga tangutica. Initially, flavonoids were enriched by means of a middle-pressure chromatographic tower (containing middle chromatogram isolated gel). In the first dimension, a XION preparative column was used to separate the flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of flavonoids and nine flavonoid fractions were obtained. Then, the coeluted flavonoid fractions were selected for further purification via reversed-phase liquid chromatography with the parent ion peak of quercetin (303), kaempferol (287), or isorhamnetin (317). Several flavonoids could be separated from each hydrophilic interaction chromatography fraction; furthermore, flavonoids with poor resolution in one-dimensional liquid chromatography were isolated in two-dimensional liquid chromatography due to the orthogonality. In addition, this technique was valuable for trace flavonoids, which were concentrated in the first stage and separated in the second stage. In total, 18 flavonoids with either quercetin, kaempferol, or isorhamnetin parent nuclei were targetedly obtained, and 15 flavonoids were obtained for the first time from S. tangutica. These results established that the off-line two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography technique was efficient for the isolation of flavonoids from Saxifraga tangutica.

Authors+Show Affiliations

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P. R. China. Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining, 810001, P. R. China.Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P. R. China. Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining, 810001, P. R. China.Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P. R. China. Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining, 810001, P. R. China.Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P. R. China. Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining, 810001, P. R. China.Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P. R. China. Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining, 810001, P. R. China.Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P. R. China. Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining, 810001, P. R. China.Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P. R. China. Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining, 810001, P. R. China.Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P. R. China. Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining, 810001, P. R. China.Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P. R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30315620

Citation

Dang, Jun, et al. "Target Separation of Flavonoids From Saxifraga Tangutica Using Two-dimensional Hydrophilic Interaction Chromatography/reversed-phase Liquid Chromatography." Journal of Separation Science, vol. 41, no. 24, 2018, pp. 4419-4429.
Dang J, Zhang L, Wang Q, et al. Target separation of flavonoids from Saxifraga tangutica using two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography. J Sep Sci. 2018;41(24):4419-4429.
Dang, J., Zhang, L., Wang, Q., Mei, L., Yue, H., Liu, Z., ... Tao, Y. (2018). Target separation of flavonoids from Saxifraga tangutica using two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography. Journal of Separation Science, 41(24), pp. 4419-4429. doi:10.1002/jssc.201800534.
Dang J, et al. Target Separation of Flavonoids From Saxifraga Tangutica Using Two-dimensional Hydrophilic Interaction Chromatography/reversed-phase Liquid Chromatography. J Sep Sci. 2018;41(24):4419-4429. PubMed PMID: 30315620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Target separation of flavonoids from Saxifraga tangutica using two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography. AU - Dang,Jun, AU - Zhang,Li, AU - Wang,Qilan, AU - Mei,Lijuan, AU - Yue,Huilan, AU - Liu,Zenggen, AU - Shao,Yun, AU - Gao,Qingbo, AU - Tao,Yanduo, Y1 - 2018/11/21/ PY - 2018/05/17/received PY - 2018/09/18/revised PY - 2018/10/09/accepted PY - 2018/10/14/pubmed PY - 2019/2/14/medline PY - 2018/10/14/entrez KW - Saxifraga tangutica KW - flavonoids KW - target separation KW - two-dimensional liquid chromatography SP - 4419 EP - 4429 JF - Journal of separation science JO - J Sep Sci VL - 41 IS - 24 N2 - An orthogonally (80.3%) preparative two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography method has been established for the isolation and purification of flavonoids from Saxifraga tangutica. Initially, flavonoids were enriched by means of a middle-pressure chromatographic tower (containing middle chromatogram isolated gel). In the first dimension, a XION preparative column was used to separate the flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of flavonoids and nine flavonoid fractions were obtained. Then, the coeluted flavonoid fractions were selected for further purification via reversed-phase liquid chromatography with the parent ion peak of quercetin (303), kaempferol (287), or isorhamnetin (317). Several flavonoids could be separated from each hydrophilic interaction chromatography fraction; furthermore, flavonoids with poor resolution in one-dimensional liquid chromatography were isolated in two-dimensional liquid chromatography due to the orthogonality. In addition, this technique was valuable for trace flavonoids, which were concentrated in the first stage and separated in the second stage. In total, 18 flavonoids with either quercetin, kaempferol, or isorhamnetin parent nuclei were targetedly obtained, and 15 flavonoids were obtained for the first time from S. tangutica. These results established that the off-line two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography technique was efficient for the isolation of flavonoids from Saxifraga tangutica. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/30315620/Target_separation_of_flavonoids_from_Saxifraga_tangutica_using_two_dimensional_hydrophilic_interaction_chromatography/reversed_phase_liquid_chromatography_ L2 - https://doi.org/10.1002/jssc.201800534 DB - PRIME DP - Unbound Medicine ER -