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Effect of n-3 long-chain polyunsaturated fatty acids supplementation in healthy mothers on DHA and EPA profiles in maternal and umbilical blood: a randomized controlled trial.
J Perinat Med. 2019 Feb 25; 47(2):200-206.JP

Abstract

Background The objective of the study was to compare the effect of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) dietary supplementation on their concentration in total lipids (TL) and lipid fractions of maternal and umbilical vein (UV) blood. The specific objective was to analyze the impact of EPA and DHA supplementation on pregnancy outcome and neonatal birth weight. Methods Women were randomly single-blinded (randomized controlled trial; ISRCTN36705743) allocated to the group receiving EPA and DHA supplementation (supplemented group) or the group receiving placebo-corn oil (control group) in the time period from January 1st, 2016 until March 1st, 2017. Women in the supplemented group (n=45) took 360 mg EPA and 240 mg DHA daily while controls (n=42) were given a placebo. Maternal and UV bloods were obtained at delivery. After lipid extraction, phospholipids (PL), cholesterol esters (CE), triacylglycerols (TG) and non-esterified fatty acids were separated by thin layer chromatography and analyzed by gas chromatography. Results Higher DHA concentrations in TL (37.24±21.87 mg/L), PL (13.14±8.07 mg/L) and triacylglycerols (2.24±2.21 mg/L) were recorded in mothers from the supplemented group when compared to the study group (TL 21.89±14.53 mg/L; P<0.001; PL 9.33±5.70 mg/L; P=0.013; TG 0.56±0.43 mg/L; P<0.001). Higher DHA concentrations in UV samples were found in TL (11.51±7.34 mg/L), PL (5.29±3.31 mg/L) and triacylglycerols (0.62±0.46 mg/L) from the supplemented groups compared with controls (TL 7.37±3.60 mg/L; P=0.002; PL 3.52±2.19 mg/L; P=0.005; TG 0.40±0.46 mg/L; P=0.035). The ratio of AA:DHA was lower in maternal (2.43) and UV serum (4.0) of the supplemented group than in the control group (maternal 3.85 P<0.001; UV 4.91 P<0.001). Conclusion The study demonstrated the higher ratio of AA/DHA in the control group indicating that pregnant women on the traditional Herzegovina diet need supplementation with DHA and EPA.

Authors+Show Affiliations

Obstetrician and Gynecologist, Department of Obstetrics and Gynecology, Mostar Clinical Hospital Center, School of Medicine, University of Mostar, Mostar, Bosnia and Herzegovina.Specialist in Obstetrics and Gynecology at Health Center, Siroki Brijeg, Bosnia and Herzegovina.Director of Ian Donald Inter-University School of Medical Ultrasound, Croatian Branch, and Specialist in Obstetrics and Gynecology at Private Clinic Veritas d.o.o, Zagreb, Croatia.Department of Obstetrics and Gynecology, Mostar Clinical Hospital Center, School of Medicine, University of Mostar, Mostar, Bosnia and Herzegovina.Department of Obstetrics and Gynecology, Mostar Clinical Hospital Center, School of Medicine, University of Mostar, Mostar, Bosnia and Herzegovina.School of Medicine, University of Mostar, Mostar, Bosnia and Herzegovina.Department of Surgery, Mostar Clinical Hospital Center, School of Medicine, University of Mostar, Mostar, Bosnia and Herzegovina.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30315737

Citation

Soldo, Dragan, et al. "Effect of N-3 Long-chain Polyunsaturated Fatty Acids Supplementation in Healthy Mothers On DHA and EPA Profiles in Maternal and Umbilical Blood: a Randomized Controlled Trial." Journal of Perinatal Medicine, vol. 47, no. 2, 2019, pp. 200-206.
Soldo D, Mikulić-Kajić M, Spalldi Barišić L, et al. Effect of n-3 long-chain polyunsaturated fatty acids supplementation in healthy mothers on DHA and EPA profiles in maternal and umbilical blood: a randomized controlled trial. J Perinat Med. 2019;47(2):200-206.
Soldo, D., Mikulić-Kajić, M., Spalldi Barišić, L., Penava, N., Orlović, M., Soldo, N., & Kajić, M. (2019). Effect of n-3 long-chain polyunsaturated fatty acids supplementation in healthy mothers on DHA and EPA profiles in maternal and umbilical blood: a randomized controlled trial. Journal of Perinatal Medicine, 47(2), 200-206. https://doi.org/10.1515/jpm-2018-0155
Soldo D, et al. Effect of N-3 Long-chain Polyunsaturated Fatty Acids Supplementation in Healthy Mothers On DHA and EPA Profiles in Maternal and Umbilical Blood: a Randomized Controlled Trial. J Perinat Med. 2019 Feb 25;47(2):200-206. PubMed PMID: 30315737.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of n-3 long-chain polyunsaturated fatty acids supplementation in healthy mothers on DHA and EPA profiles in maternal and umbilical blood: a randomized controlled trial. AU - Soldo,Dragan, AU - Mikulić-Kajić,Matija, AU - Spalldi Barišić,Lara, AU - Penava,Nikolina, AU - Orlović,Martina, AU - Soldo,Neven, AU - Kajić,Martin, PY - 2018/05/03/received PY - 2018/09/14/accepted PY - 2018/10/14/pubmed PY - 2019/5/18/medline PY - 2018/10/14/entrez KW - fetus KW - maternal n-3 LCPUFA supplementation KW - pregnancy SP - 200 EP - 206 JF - Journal of perinatal medicine JO - J Perinat Med VL - 47 IS - 2 N2 - Background The objective of the study was to compare the effect of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) dietary supplementation on their concentration in total lipids (TL) and lipid fractions of maternal and umbilical vein (UV) blood. The specific objective was to analyze the impact of EPA and DHA supplementation on pregnancy outcome and neonatal birth weight. Methods Women were randomly single-blinded (randomized controlled trial; ISRCTN36705743) allocated to the group receiving EPA and DHA supplementation (supplemented group) or the group receiving placebo-corn oil (control group) in the time period from January 1st, 2016 until March 1st, 2017. Women in the supplemented group (n=45) took 360 mg EPA and 240 mg DHA daily while controls (n=42) were given a placebo. Maternal and UV bloods were obtained at delivery. After lipid extraction, phospholipids (PL), cholesterol esters (CE), triacylglycerols (TG) and non-esterified fatty acids were separated by thin layer chromatography and analyzed by gas chromatography. Results Higher DHA concentrations in TL (37.24±21.87 mg/L), PL (13.14±8.07 mg/L) and triacylglycerols (2.24±2.21 mg/L) were recorded in mothers from the supplemented group when compared to the study group (TL 21.89±14.53 mg/L; P<0.001; PL 9.33±5.70 mg/L; P=0.013; TG 0.56±0.43 mg/L; P<0.001). Higher DHA concentrations in UV samples were found in TL (11.51±7.34 mg/L), PL (5.29±3.31 mg/L) and triacylglycerols (0.62±0.46 mg/L) from the supplemented groups compared with controls (TL 7.37±3.60 mg/L; P=0.002; PL 3.52±2.19 mg/L; P=0.005; TG 0.40±0.46 mg/L; P=0.035). The ratio of AA:DHA was lower in maternal (2.43) and UV serum (4.0) of the supplemented group than in the control group (maternal 3.85 P<0.001; UV 4.91 P<0.001). Conclusion The study demonstrated the higher ratio of AA/DHA in the control group indicating that pregnant women on the traditional Herzegovina diet need supplementation with DHA and EPA. SN - 1619-3997 UR - https://www.unboundmedicine.com/medline/citation/30315737/Effect_of_n_3_long_chain_polyunsaturated_fatty_acids_supplementation_in_healthy_mothers_on_DHA_and_EPA_profiles_in_maternal_and_umbilical_blood:_a_randomized_controlled_trial_ L2 - https://www.degruyter.com/document/doi/10.1515/jpm-2018-0155 DB - PRIME DP - Unbound Medicine ER -