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Detection of biogenic amines in pet food ingredients by RP-HPLC with automated dansyl chloride derivatization.
J Sep Sci. 2018 Dec; 41(24):4430-4436.JS

Abstract

The detection of biogenic amines is of significant interest to the food industry, as they can be used as indicators of food spoilage and they are potentially toxic. Because of their importance, there is a need for automated methods suitable for industry use that can detect a wide range of biogenic amines at sufficient levels for food analysis. In this work, optimized conditions for the automated determination of biogenic amines (histamine, putrescine, cadaverine, spermine, spermidine, tyramine, and tryptamine) derivatized with dansyl chloride are presented. Limits of detection below 0.2 ppm were achieved for seven biogenic amines and percentage recoveries were between 80 and 109% for the seven analytes spiked into meat meal samples. The method is simple and compared well to an existing method for the detection of biogenic amines in pet food ingredients.

Authors+Show Affiliations

School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Australia. Kemin Nutrisurance, Geelong Technology Precinct, Waurn Ponds, Australia.Kemin Nutrisurance, Geelong Technology Precinct, Waurn Ponds, Australia.Kemin Nutrisurance, Des Moines, Iowa, USA.School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Australia.School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30318732

Citation

Learey, Jessica J., et al. "Detection of Biogenic Amines in Pet Food Ingredients By RP-HPLC With Automated Dansyl Chloride Derivatization." Journal of Separation Science, vol. 41, no. 24, 2018, pp. 4430-4436.
Learey JJ, Crawford-Clark S, Bowen BJ, et al. Detection of biogenic amines in pet food ingredients by RP-HPLC with automated dansyl chloride derivatization. J Sep Sci. 2018;41(24):4430-4436.
Learey, J. J., Crawford-Clark, S., Bowen, B. J., Barrow, C. J., & Adcock, J. L. (2018). Detection of biogenic amines in pet food ingredients by RP-HPLC with automated dansyl chloride derivatization. Journal of Separation Science, 41(24), 4430-4436. https://doi.org/10.1002/jssc.201800455
Learey JJ, et al. Detection of Biogenic Amines in Pet Food Ingredients By RP-HPLC With Automated Dansyl Chloride Derivatization. J Sep Sci. 2018;41(24):4430-4436. PubMed PMID: 30318732.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of biogenic amines in pet food ingredients by RP-HPLC with automated dansyl chloride derivatization. AU - Learey,Jessica J, AU - Crawford-Clark,Shona, AU - Bowen,Benjamin J, AU - Barrow,Colin J, AU - Adcock,Jacqui L, Y1 - 2018/11/02/ PY - 2018/04/26/received PY - 2018/09/13/revised PY - 2018/10/09/accepted PY - 2018/10/16/pubmed PY - 2019/2/14/medline PY - 2018/10/16/entrez KW - automation KW - biogenic amines KW - dansyl chloride KW - derivatization KW - pet food SP - 4430 EP - 4436 JF - Journal of separation science JO - J Sep Sci VL - 41 IS - 24 N2 - The detection of biogenic amines is of significant interest to the food industry, as they can be used as indicators of food spoilage and they are potentially toxic. Because of their importance, there is a need for automated methods suitable for industry use that can detect a wide range of biogenic amines at sufficient levels for food analysis. In this work, optimized conditions for the automated determination of biogenic amines (histamine, putrescine, cadaverine, spermine, spermidine, tyramine, and tryptamine) derivatized with dansyl chloride are presented. Limits of detection below 0.2 ppm were achieved for seven biogenic amines and percentage recoveries were between 80 and 109% for the seven analytes spiked into meat meal samples. The method is simple and compared well to an existing method for the detection of biogenic amines in pet food ingredients. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/30318732/Detection_of_biogenic_amines_in_pet_food_ingredients_by_RP_HPLC_with_automated_dansyl_chloride_derivatization_ L2 - https://doi.org/10.1002/jssc.201800455 DB - PRIME DP - Unbound Medicine ER -