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Pgrmc1 Knockout Impairs Oocyte Maturation in Zebrafish.

Abstract

Recent investigations suggest progestin receptor membrane component 1 (PGRMC1) associates with and transports a wide range of molecules such as heme, cytochromes P450, steroids with 21 carbons, membrane progestin receptor alpha (mPRα/Paqr7), epidermal growth factor receptor (EGFR), and insulin receptor. It is difficult to discriminate the true functions of PGRMC1 from the functions of its associated molecules using biochemical and pharmacological approaches. To determine the physiological function(s) of PGRMC1, we generated global knockouts for pgrmc1 (pgrmc1-/-) in zebrafish. We found a reduction in both spawning frequency and the number of embryos produced by female mutants. We also observed reduced sensitivity of fully-grown immature oocytes to a progestin hormone and a reduced number of oocytes undergone meiotic maturation both in vivo and in vitro in pgrmc1-/-. This reduced sensitivity to progestin corresponds well with significant reduced expression of mPRα, the receptor mainly responsible for mediating oocyte maturation and meiosis resumption in fish. The results provide in vivo and in vitro evidence for the physiological functions of Pgrmc1 in oocyte maturation and fertility, as well as a plausible molecular mechanism via regulation of mPRα, which in turn directly regulates oocyte maturation and affects fertility in zebrafish.

Authors+Show Affiliations

Department of Biology, East Carolina University, Greenville, NC, United States.Marine Science Institute, University of Texas at Austin, Port Aransas, TX, United States.Department of Biology, East Carolina University, Greenville, NC, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30319543

Citation

Wu, Xin-Jun, et al. "Pgrmc1 Knockout Impairs Oocyte Maturation in Zebrafish." Frontiers in Endocrinology, vol. 9, 2018, p. 560.
Wu XJ, Thomas P, Zhu Y. Pgrmc1 Knockout Impairs Oocyte Maturation in Zebrafish. Front Endocrinol (Lausanne). 2018;9:560.
Wu, X. J., Thomas, P., & Zhu, Y. (2018). Pgrmc1 Knockout Impairs Oocyte Maturation in Zebrafish. Frontiers in Endocrinology, 9, p. 560. doi:10.3389/fendo.2018.00560.
Wu XJ, Thomas P, Zhu Y. Pgrmc1 Knockout Impairs Oocyte Maturation in Zebrafish. Front Endocrinol (Lausanne). 2018;9:560. PubMed PMID: 30319543.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pgrmc1 Knockout Impairs Oocyte Maturation in Zebrafish. AU - Wu,Xin-Jun, AU - Thomas,Peter, AU - Zhu,Yong, Y1 - 2018/09/24/ PY - 2018/05/25/received PY - 2018/09/03/accepted PY - 2018/10/16/entrez PY - 2018/10/16/pubmed PY - 2018/10/16/medline KW - Paqr7 KW - Pgrmc1 KW - mPRα KW - oocyte maturation KW - progestin KW - subfertility SP - 560 EP - 560 JF - Frontiers in endocrinology JO - Front Endocrinol (Lausanne) VL - 9 N2 - Recent investigations suggest progestin receptor membrane component 1 (PGRMC1) associates with and transports a wide range of molecules such as heme, cytochromes P450, steroids with 21 carbons, membrane progestin receptor alpha (mPRα/Paqr7), epidermal growth factor receptor (EGFR), and insulin receptor. It is difficult to discriminate the true functions of PGRMC1 from the functions of its associated molecules using biochemical and pharmacological approaches. To determine the physiological function(s) of PGRMC1, we generated global knockouts for pgrmc1 (pgrmc1-/-) in zebrafish. We found a reduction in both spawning frequency and the number of embryos produced by female mutants. We also observed reduced sensitivity of fully-grown immature oocytes to a progestin hormone and a reduced number of oocytes undergone meiotic maturation both in vivo and in vitro in pgrmc1-/-. This reduced sensitivity to progestin corresponds well with significant reduced expression of mPRα, the receptor mainly responsible for mediating oocyte maturation and meiosis resumption in fish. The results provide in vivo and in vitro evidence for the physiological functions of Pgrmc1 in oocyte maturation and fertility, as well as a plausible molecular mechanism via regulation of mPRα, which in turn directly regulates oocyte maturation and affects fertility in zebrafish. SN - 1664-2392 UR - https://www.unboundmedicine.com/medline/citation/30319543/Pgrmc1_Knockout_Impairs_Oocyte_Maturation_in_Zebrafish_ L2 - https://dx.doi.org/10.3389/fendo.2018.00560 DB - PRIME DP - Unbound Medicine ER -