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Associations of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms with Ischemic Stroke in the Northern Chinese Han Population.
Med Sci Monit. 2018 Oct 15; 24:7366-7374.MS

Abstract

BACKGROUND

Recently, miR-146a C>G, miR- 149 T>C, miR-196a2 T>C and miR-499 A>G polymorphisms have been associated with susceptibility to many diseases, including ischemic stroke (IS). However, results have been reported inconsistency in IS, especially in the Chinese population. This study aimed to investigate the polymorphisms of the 4 miRNAs and IS risk in the Chinese population. MATERIAL AND

METHODS

We used a case-control study to explore these associations in 396 patients with IS and 378 healthy controls. According to TOAST standards, the selected patients were divided into subgroups: the large artery atherosclerosis (LAA) subgroup and the small artery occlusion (SAO) subgroup. The method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotypes.

RESULTS

The miR-146a C>G polymorphism was remarkably different (CC vs. CG+GG: P=0.027; CC+CG vs. GG: P=0.020; C vs. G: P=0.006). The miR-149 T>C polymorphism was also remarkably different (TT vs. TC+CC: P=0.017; TT+TC vs. CC: P=0.020; T vs. C: P=0.004). The miR-146a and miR-149 polymorphisms were also remarkably different in the LAA subgroup (P<0.05). However, we did not find an association of miR-196a2 T>C or miR-499 A>G polymorphisms with IS (P>0.05); we did not find any association in the LAA subgroup or the SAO subgroup (P>0.05).

CONCLUSIONS

Our study suggested that miR-146a C>G and miR-149 T>C polymorphisms might remarkably increase the risk of IS, which might be mainly associated with an increased risk in LAA stroke; however, the miR-196a2 T>C and miR-499 A>G polymorphisms might not be associated with IS risk in the northern Chinese Han population.

Authors+Show Affiliations

Department of Critical Care Medicine, The Affiliated Hiser Hospital of Qingdao University, Qingdao, Shandong, China (mainland).Department of Neurology, The Affiliated Hiser Hospital of Qingdao University, Qingdao, Shandong, China (mainland).Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China (mainland).Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China (mainland).Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China (mainland).

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30321140

Citation

Zhu, Xiaoyan, et al. "Associations of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms With Ischemic Stroke in the Northern Chinese Han Population." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 24, 2018, pp. 7366-7374.
Zhu X, Hou R, Ma A, et al. Associations of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms with Ischemic Stroke in the Northern Chinese Han Population. Med Sci Monit. 2018;24:7366-7374.
Zhu, X., Hou, R., Ma, A., Yang, S., & Pan, X. (2018). Associations of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms with Ischemic Stroke in the Northern Chinese Han Population. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 7366-7374. https://doi.org/10.12659/MSM.909935
Zhu X, et al. Associations of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms With Ischemic Stroke in the Northern Chinese Han Population. Med Sci Monit. 2018 Oct 15;24:7366-7374. PubMed PMID: 30321140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Associations of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms with Ischemic Stroke in the Northern Chinese Han Population. AU - Zhu,Xiaoyan, AU - Hou,Rongyao, AU - Ma,Aijun, AU - Yang,Shaonan, AU - Pan,Xudong, Y1 - 2018/10/15/ PY - 2018/10/16/entrez PY - 2018/10/16/pubmed PY - 2018/12/12/medline SP - 7366 EP - 7374 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med Sci Monit VL - 24 N2 - BACKGROUND Recently, miR-146a C>G, miR- 149 T>C, miR-196a2 T>C and miR-499 A>G polymorphisms have been associated with susceptibility to many diseases, including ischemic stroke (IS). However, results have been reported inconsistency in IS, especially in the Chinese population. This study aimed to investigate the polymorphisms of the 4 miRNAs and IS risk in the Chinese population. MATERIAL AND METHODS We used a case-control study to explore these associations in 396 patients with IS and 378 healthy controls. According to TOAST standards, the selected patients were divided into subgroups: the large artery atherosclerosis (LAA) subgroup and the small artery occlusion (SAO) subgroup. The method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotypes. RESULTS The miR-146a C>G polymorphism was remarkably different (CC vs. CG+GG: P=0.027; CC+CG vs. GG: P=0.020; C vs. G: P=0.006). The miR-149 T>C polymorphism was also remarkably different (TT vs. TC+CC: P=0.017; TT+TC vs. CC: P=0.020; T vs. C: P=0.004). The miR-146a and miR-149 polymorphisms were also remarkably different in the LAA subgroup (P<0.05). However, we did not find an association of miR-196a2 T>C or miR-499 A>G polymorphisms with IS (P>0.05); we did not find any association in the LAA subgroup or the SAO subgroup (P>0.05). CONCLUSIONS Our study suggested that miR-146a C>G and miR-149 T>C polymorphisms might remarkably increase the risk of IS, which might be mainly associated with an increased risk in LAA stroke; however, the miR-196a2 T>C and miR-499 A>G polymorphisms might not be associated with IS risk in the northern Chinese Han population. SN - 1643-3750 UR - https://www.unboundmedicine.com/medline/citation/30321140/Associations_of_miR_146a_miR_149_miR_196a2_and_miR_499_Polymorphisms_with_Ischemic_Stroke_in_the_Northern_Chinese_Han_Population_ L2 - https://www.medscimonit.com/download/index/idArt/909935 DB - PRIME DP - Unbound Medicine ER -