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Impact of a (poly)phenol-rich extract from the brown algae Ascophyllum nodosum on DNA damage and antioxidant activity in an overweight or obese population: a randomized controlled trial.
Am J Clin Nutr 2018; 108(4):688-700AJ

Abstract

Background

Epidemiologic evidence suggests that a diet rich in (poly)phenols has beneficial effects on many chronic diseases. Brown seaweed is a rich source of (poly)phenols.

Objective

The aim of this study was to investigate the bioavailability and effect of a brown seaweed (Ascophyllum nodosum) (poly)phenol extract on DNA damage, oxidative stress, and inflammation in vivo.

Design

A randomized, double-blind, placebo-controlled crossover trial was conducted in 80 participants aged 30-65 y with a body mass index (in kg/m2) ≥25. The participants consumed either a 400-mg capsule containing 100 mg seaweed (poly)phenol and 300 mg maltodextrin or a 400-mg maltodextrin placebo control capsule daily for an 8-wk period. Bioactivity was assessed with a panel of blood-based markers including lymphocyte DNA damage, plasma oxidant capacity, C-reactive protein (CRP), and inflammatory cytokines. To explore the bioavailability of seaweed phenolics, an untargeted metabolomics analysis of urine and plasma samples after seaweed consumption was determined by ultra-high-performance liquid chromatography-high-resolution mass spectrometry.

Results

Consumption of the seaweed (poly)phenols resulted in a modest decrease in DNA damage but only in a subset of the total population who were obese. There were no significant changes in CRP, antioxidant status, or inflammatory cytokines. We identified phlorotannin metabolites that are considered potential biomarkers of seaweed consumption including pyrogallol/phloroglucinol-sulfate, hydroxytrifurahol A-glucuronide, dioxinodehydroeckol-glucuronide, diphlorethol sulfates, C-O-C dimer of phloroglucinol sulfate, and C-O-C dimer of phloroglucinol.

Conclusions

To the best of our knowledge, this work represents the first comprehensive study investigating the bioactivity and bioavailability of seaweed (poly)phenolics in human participants. We identified several potential biomarkers of seaweed consumption. Intriguingly, the modest improvements in DNA damage were observed only in the obese subset of the total population. The subgroup analysis should be considered exploratory because it was not preplanned; therefore, it was not powered adequately. Elucidation of the biology underpinning this observation will require participant stratification according to weight in future studies. This trial was registered at clinicaltrials.gov as NCT02295878.

Authors+Show Affiliations

Nutrition Innovation Centre for Food and Health, University of Ulster, Coleraine, United Kingdom.Nutrition Innovation Centre for Food and Health, University of Ulster, Coleraine, United Kingdom.Nutrition Innovation Centre for Food and Health, University of Ulster, Coleraine, United Kingdom.Nutrition Innovation Centre for Food and Health, University of Ulster, Coleraine, United Kingdom.Nutrition Innovation Centre for Food and Health, University of Ulster, Coleraine, United Kingdom.Nutrition Innovation Centre for Food and Health, University of Ulster, Coleraine, United Kingdom.School of Biomedical Sciences, Centre for Molecular Biosciences, University of Ulster, Coleraine, United Kingdom.Nutrition Innovation Centre for Food and Health, University of Ulster, Coleraine, United Kingdom.Nutrition Innovation Centre for Food and Health, University of Ulster, Coleraine, United Kingdom.Nutrition Innovation Centre for Food and Health, University of Ulster, Coleraine, United Kingdom.Nutrition Innovation Centre for Food and Health, University of Ulster, Coleraine, United Kingdom.Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom. Health Sciences Research Centre, University of Roehampton, London, United Kingdom.Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom.Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom.CyberColloids Ltd., Carrigaline Industrial Estate, Carrigaline, Ireland.CyberColloids Ltd., Carrigaline Industrial Estate, Carrigaline, Ireland.Department of Food Science and Health, IFAPA-Alameda del Obispo, Córdoba, Spain.Department of Food Science and Health, IFAPA-Alameda del Obispo, Córdoba, Spain.Department of Food Science and Health, IFAPA-Alameda del Obispo, Córdoba, Spain.Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom.Nutrition Innovation Centre for Food and Health, University of Ulster, Coleraine, United Kingdom.

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30321272

Citation

Baldrick, Francina R., et al. "Impact of a (poly)phenol-rich Extract From the Brown Algae Ascophyllum Nodosum On DNA Damage and Antioxidant Activity in an Overweight or Obese Population: a Randomized Controlled Trial." The American Journal of Clinical Nutrition, vol. 108, no. 4, 2018, pp. 688-700.
Baldrick FR, McFadden K, Ibars M, et al. Impact of a (poly)phenol-rich extract from the brown algae Ascophyllum nodosum on DNA damage and antioxidant activity in an overweight or obese population: a randomized controlled trial. Am J Clin Nutr. 2018;108(4):688-700.
Baldrick, F. R., McFadden, K., Ibars, M., Sung, C., Moffatt, T., Megarry, K., ... Gill, C. I. R. (2018). Impact of a (poly)phenol-rich extract from the brown algae Ascophyllum nodosum on DNA damage and antioxidant activity in an overweight or obese population: a randomized controlled trial. The American Journal of Clinical Nutrition, 108(4), pp. 688-700. doi:10.1093/ajcn/nqy147.
Baldrick FR, et al. Impact of a (poly)phenol-rich Extract From the Brown Algae Ascophyllum Nodosum On DNA Damage and Antioxidant Activity in an Overweight or Obese Population: a Randomized Controlled Trial. Am J Clin Nutr. 2018 10 1;108(4):688-700. PubMed PMID: 30321272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of a (poly)phenol-rich extract from the brown algae Ascophyllum nodosum on DNA damage and antioxidant activity in an overweight or obese population: a randomized controlled trial. AU - Baldrick,Francina R, AU - McFadden,Kevin, AU - Ibars,Maria, AU - Sung,Chris, AU - Moffatt,Tanya, AU - Megarry,Kate, AU - Thomas,Keith, AU - Mitchell,Peter, AU - Wallace,Julie M W, AU - Pourshahidi,L Kirsty, AU - Ternan,Nigel G, AU - Corona,Giulia, AU - Spencer,Jeremy, AU - Yaqoob,Parveen, AU - Hotchkiss,Sarah, AU - Campbell,Ross, AU - Moreno-Rojas,José Manuel, AU - Cuevas,Francisco Julián, AU - Pereira-Caro,Gema, AU - Rowland,Ian, AU - Gill,Chris I R, PY - 2017/09/05/received PY - 2018/06/15/accepted PY - 2018/10/16/entrez PY - 2018/10/16/pubmed PY - 2019/8/20/medline SP - 688 EP - 700 JF - The American journal of clinical nutrition JO - Am. J. Clin. Nutr. VL - 108 IS - 4 N2 - Background: Epidemiologic evidence suggests that a diet rich in (poly)phenols has beneficial effects on many chronic diseases. Brown seaweed is a rich source of (poly)phenols. Objective: The aim of this study was to investigate the bioavailability and effect of a brown seaweed (Ascophyllum nodosum) (poly)phenol extract on DNA damage, oxidative stress, and inflammation in vivo. Design: A randomized, double-blind, placebo-controlled crossover trial was conducted in 80 participants aged 30-65 y with a body mass index (in kg/m2) ≥25. The participants consumed either a 400-mg capsule containing 100 mg seaweed (poly)phenol and 300 mg maltodextrin or a 400-mg maltodextrin placebo control capsule daily for an 8-wk period. Bioactivity was assessed with a panel of blood-based markers including lymphocyte DNA damage, plasma oxidant capacity, C-reactive protein (CRP), and inflammatory cytokines. To explore the bioavailability of seaweed phenolics, an untargeted metabolomics analysis of urine and plasma samples after seaweed consumption was determined by ultra-high-performance liquid chromatography-high-resolution mass spectrometry. Results: Consumption of the seaweed (poly)phenols resulted in a modest decrease in DNA damage but only in a subset of the total population who were obese. There were no significant changes in CRP, antioxidant status, or inflammatory cytokines. We identified phlorotannin metabolites that are considered potential biomarkers of seaweed consumption including pyrogallol/phloroglucinol-sulfate, hydroxytrifurahol A-glucuronide, dioxinodehydroeckol-glucuronide, diphlorethol sulfates, C-O-C dimer of phloroglucinol sulfate, and C-O-C dimer of phloroglucinol. Conclusions: To the best of our knowledge, this work represents the first comprehensive study investigating the bioactivity and bioavailability of seaweed (poly)phenolics in human participants. We identified several potential biomarkers of seaweed consumption. Intriguingly, the modest improvements in DNA damage were observed only in the obese subset of the total population. The subgroup analysis should be considered exploratory because it was not preplanned; therefore, it was not powered adequately. Elucidation of the biology underpinning this observation will require participant stratification according to weight in future studies. This trial was registered at clinicaltrials.gov as NCT02295878. SN - 1938-3207 UR - https://www.unboundmedicine.com/medline/citation/30321272/Impact_of_a__poly_phenol_rich_extract_from_the_brown_algae_Ascophyllum_nodosum_on_DNA_damage_and_antioxidant_activity_in_an_overweight_or_obese_population:_a_randomized_controlled_trial_ L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.1093/ajcn/nqy147 DB - PRIME DP - Unbound Medicine ER -