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Differences in Tissue Distribution of Cyano⁻B12 and Hydroxo⁻B12 One Week after Oral Intake: An Experimental Study in Male Wistar Rats.
Nutrients. 2018 Oct 12; 10(10)N

Abstract

Foods contain natural vitamin B12 forms, such as hydroxo⁻B12 (HO⁻B12), whereas vitamin pills contain the synthetic cyano⁻B12 (CN⁻B12). Recent studies in rats showed different tissue distributions of CN⁻B12 and HO⁻B12 24 h after oral administration. Here, we investigate whether these differences are sustained or leveled out with time in both B12-deplete and -replete rats, thereby assessing if the two forms are equally good at maintaining a normal B12 status. Male Wistar rats were fed diets with low (n = 16) or high (n = 12) B12 content for 17 days. At day 10, the rats received a single oral dose of [57Co]-labeled CN⁻B12 or HO⁻B12 (n = 6 and n = 8, respectively, in each diet group). The rats were sacrificed on day 17 and endogenous B12 and [57Co]⁻B12 were measured in liver, kidney, and plasma. We found that the low-B12 diet introduced a B12-deplete state as judged from medians of endogenous B12 compared to rats on a (high-B12 diet): Plasma (565 (1410) pmol/L), liver (28.2 (33.2) pmol/g), and kidneys (123 (1300) pmol/g). One week after oral administration, the labeled B12 was distributed as follows: HO⁻B12 > CN⁻B12 (liver) and CN⁻B12 > HO⁻B12 (kidneys, plasma). The tissue/plasma ratios showed different equilibriums for labeled CN⁻B12 and HO⁻B12 in the B12-deplete and -replete groups. The equilibrium of endogenous B12 resembled [57Co]CN⁻B12 in replete rats but differed from both [57Co]CN⁻B12 and [57Co]HO⁻B12 in deplete rats. The data suggest long-term differences in tissue utilization of the two B12 forms and warrant further studies concerning the possible benefits of consuming HO⁻B12 instead of CN⁻B12 in oral B12 replacement.

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Authors+Show Affiliations

Greibe E
Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark. greibe@clin.au.dk.
Nymark O
Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark. au335591@post.au.dk.
Fedosov SN
Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus, Denmark. SNFedosov1960@gmail.com.
Heegaard CW
Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus, Denmark. cwh@mbg.au.dk.
Nexo E
Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark. ebbanexo@rm.dk.

MeSH

Administration, OralAnimalsHydroxocobalaminKidneyLiverMalePlasmaRats, WistarTissue DistributionVitamin B 12Vitamin B 12 Deficiency

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30322035

Citation

Greibe, Eva, et al. "Differences in Tissue Distribution of Cyano⁻B12 and Hydroxo⁻B12 One Week After Oral Intake: an Experimental Study in Male Wistar Rats." Nutrients, vol. 10, no. 10, 2018.
Greibe E, Nymark O, Fedosov SN, et al. Differences in Tissue Distribution of Cyano⁻B12 and Hydroxo⁻B12 One Week after Oral Intake: An Experimental Study in Male Wistar Rats. Nutrients. 2018;10(10).
Greibe, E., Nymark, O., Fedosov, S. N., Heegaard, C. W., & Nexo, E. (2018). Differences in Tissue Distribution of Cyano⁻B12 and Hydroxo⁻B12 One Week after Oral Intake: An Experimental Study in Male Wistar Rats. Nutrients, 10(10). https://doi.org/10.3390/nu10101487
Greibe E, et al. Differences in Tissue Distribution of Cyano⁻B12 and Hydroxo⁻B12 One Week After Oral Intake: an Experimental Study in Male Wistar Rats. Nutrients. 2018 Oct 12;10(10) PubMed PMID: 30322035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differences in Tissue Distribution of Cyano⁻B12 and Hydroxo⁻B12 One Week after Oral Intake: An Experimental Study in Male Wistar Rats. AU - Greibe,Eva, AU - Nymark,Ole, AU - Fedosov,Sergey N, AU - Heegaard,Christian W, AU - Nexo,Ebba, Y1 - 2018/10/12/ PY - 2018/09/06/received PY - 2018/10/04/revised PY - 2018/10/10/accepted PY - 2018/10/17/entrez PY - 2018/10/17/pubmed PY - 2019/1/4/medline KW - cobalamin KW - cyano–B12 KW - hydroxo–B12 KW - rats KW - tissue distribution KW - vitamin B12 JF - Nutrients JO - Nutrients VL - 10 IS - 10 N2 - Foods contain natural vitamin B12 forms, such as hydroxo⁻B12 (HO⁻B12), whereas vitamin pills contain the synthetic cyano⁻B12 (CN⁻B12). Recent studies in rats showed different tissue distributions of CN⁻B12 and HO⁻B12 24 h after oral administration. Here, we investigate whether these differences are sustained or leveled out with time in both B12-deplete and -replete rats, thereby assessing if the two forms are equally good at maintaining a normal B12 status. Male Wistar rats were fed diets with low (n = 16) or high (n = 12) B12 content for 17 days. At day 10, the rats received a single oral dose of [57Co]-labeled CN⁻B12 or HO⁻B12 (n = 6 and n = 8, respectively, in each diet group). The rats were sacrificed on day 17 and endogenous B12 and [57Co]⁻B12 were measured in liver, kidney, and plasma. We found that the low-B12 diet introduced a B12-deplete state as judged from medians of endogenous B12 compared to rats on a (high-B12 diet): Plasma (565 (1410) pmol/L), liver (28.2 (33.2) pmol/g), and kidneys (123 (1300) pmol/g). One week after oral administration, the labeled B12 was distributed as follows: HO⁻B12 > CN⁻B12 (liver) and CN⁻B12 > HO⁻B12 (kidneys, plasma). The tissue/plasma ratios showed different equilibriums for labeled CN⁻B12 and HO⁻B12 in the B12-deplete and -replete groups. The equilibrium of endogenous B12 resembled [57Co]CN⁻B12 in replete rats but differed from both [57Co]CN⁻B12 and [57Co]HO⁻B12 in deplete rats. The data suggest long-term differences in tissue utilization of the two B12 forms and warrant further studies concerning the possible benefits of consuming HO⁻B12 instead of CN⁻B12 in oral B12 replacement. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/30322035/Differences_in_Tissue_Distribution_of_Cyano⁻B12_and_Hydroxo⁻B12_One_Week_after_Oral_Intake:_An_Experimental_Study_in_Male_Wistar_Rats_ DB - PRIME DP - Unbound Medicine ER -