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Ultrasensitive detection of glutathione based on liquid crystals in the presence of γ-glutamyl transpeptidase.
Anal Chim Acta 2018; 1040:187-195AC

Abstract

Glutathione (GSH) is of significance in human pathology due to its many cellular functions. γ-Glutamyl transpeptidase (γ-GT) plays a key role in the balance of GSH by breaking down extracellular GSH. In this study, we proposed a novel strategy for sensitive and selective detection of GSH based on LC sensing platform coupled with its inhibition of γ-GT. The γ-GT cleavable surfactant, dodecyl N-ethanamide N, N, N-trimethylammonium bromide (DAA), was synthesized and utilized to decorate the aqueous/LC interface. A black optical image of LCs was observed due to formation of the self-assembled DAA monolayer at the interface. Subsequently, the optical response of LCs returned to bright appearance after transferring γ-GT to the DAA-laden interface because DAA could be enzymatically hydrolyzed by γ-GT and the monolayer was disrupted. Interestingly, LCs remained dark images after the DAA-decorated interface in contact with the pre-incubated GSH and γ-GT. The detection limit of GSH reached 1 pg/mL, which demonstrates high competitiveness with the current assays. The detection mechanism of GSH was explained by Isothermal titration calorimetry (ITC) measurements and Fourier transform infrared (FTIR) spectroscopy. This work presents an appealing route to achieve ultrasensitive detection of GSH according to its hydrolysis by γ-GT and the as-prepared simple and robust LC-based sensing platform has potentials in the diagnosis of GSH-related diseases (e.g. cancer, liver damage, and malignant neoplasms).

Authors+Show Affiliations

Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan, 250100, PR China; School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165, PR China.College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014, PR China.Department of Chemistry, University of Houston, Houston, TX, 77204, United States.Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan, 250100, PR China; School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165, PR China. Electronic address: ylmlt@sdu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30327109

Citation

Zhou, Lele, et al. "Ultrasensitive Detection of Glutathione Based On Liquid Crystals in the Presence of Γ-glutamyl Transpeptidase." Analytica Chimica Acta, vol. 1040, 2018, pp. 187-195.
Zhou L, Kang Q, Hu O, et al. Ultrasensitive detection of glutathione based on liquid crystals in the presence of γ-glutamyl transpeptidase. Anal Chim Acta. 2018;1040:187-195.
Zhou, L., Kang, Q., Hu, O., & Yu, L. (2018). Ultrasensitive detection of glutathione based on liquid crystals in the presence of γ-glutamyl transpeptidase. Analytica Chimica Acta, 1040, pp. 187-195. doi:10.1016/j.aca.2018.08.029.
Zhou L, et al. Ultrasensitive Detection of Glutathione Based On Liquid Crystals in the Presence of Γ-glutamyl Transpeptidase. Anal Chim Acta. 2018 Dec 21;1040:187-195. PubMed PMID: 30327109.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasensitive detection of glutathione based on liquid crystals in the presence of γ-glutamyl transpeptidase. AU - Zhou,Lele, AU - Kang,Qi, AU - Hu,Oiongzheng, AU - Yu,Li, Y1 - 2018/08/15/ PY - 2018/06/12/received PY - 2018/08/08/revised PY - 2018/08/13/accepted PY - 2018/10/18/entrez PY - 2018/10/18/pubmed PY - 2019/1/15/medline KW - Glutathione KW - Hydrolysis KW - Liquid crystal KW - Surfactant KW - γ-Glutamyl transpeptidase SP - 187 EP - 195 JF - Analytica chimica acta JO - Anal. Chim. Acta VL - 1040 N2 - Glutathione (GSH) is of significance in human pathology due to its many cellular functions. γ-Glutamyl transpeptidase (γ-GT) plays a key role in the balance of GSH by breaking down extracellular GSH. In this study, we proposed a novel strategy for sensitive and selective detection of GSH based on LC sensing platform coupled with its inhibition of γ-GT. The γ-GT cleavable surfactant, dodecyl N-ethanamide N, N, N-trimethylammonium bromide (DAA), was synthesized and utilized to decorate the aqueous/LC interface. A black optical image of LCs was observed due to formation of the self-assembled DAA monolayer at the interface. Subsequently, the optical response of LCs returned to bright appearance after transferring γ-GT to the DAA-laden interface because DAA could be enzymatically hydrolyzed by γ-GT and the monolayer was disrupted. Interestingly, LCs remained dark images after the DAA-decorated interface in contact with the pre-incubated GSH and γ-GT. The detection limit of GSH reached 1 pg/mL, which demonstrates high competitiveness with the current assays. The detection mechanism of GSH was explained by Isothermal titration calorimetry (ITC) measurements and Fourier transform infrared (FTIR) spectroscopy. This work presents an appealing route to achieve ultrasensitive detection of GSH according to its hydrolysis by γ-GT and the as-prepared simple and robust LC-based sensing platform has potentials in the diagnosis of GSH-related diseases (e.g. cancer, liver damage, and malignant neoplasms). SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/30327109/Ultrasensitive_detection_of_glutathione_based_on_liquid_crystals_in_the_presence_of_γ_glutamyl_transpeptidase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(18)30983-8 DB - PRIME DP - Unbound Medicine ER -