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Anti-hyperuricemic and nephroprotective effects of extracts from Chaenomeles sinensis (Thouin) Koehne in hyperuricemic mice.
Food Funct. 2018 Nov 14; 9(11):5778-5790.FF

Abstract

Clinically, Chaenomeles sinensis (Thouin) Koehne (C. sinensis) has been used to treat hyperuricemia and gout. However, the exact mechanism of action is still unknown. In the present study, the ethyl acetate fraction of C. sinensis fruit extract (CSF-E) was separated. Potassium oxonate (PO)-induced hyperuricemic mice and normal mice were administered with CSF-E at 60, 120 and 180 mg kg-1, respectively for 7 days. Serum uric acid, creatinine and BUN levels, liver oxidative damage, and serum and hepatic XOD activities were primarily measured using assay kits. The evaluation of its nephroprotective effects was carried out by renal histopathological analysis. Simultaneously, renal protein levels of organic anion transporters, such as mURAT1 and mOAT1, were detected using western blotting to elucidate the possible mechanisms. The results showed that CSF-E could significantly inhibit XOD activities in both serum and liver (p < 0.05), decreasing uric acid, creatinine and BUN levels in serum, and increasing levels in the excretion of uric acid by down-regulated of mURAT1 and up-regulated mOAT1 protein expression of kidney in hyperuricemic mice. Moreover, PO-induced alterations in the levels of MDA, hepatic SOD and GSH-Px activities and renal inflammation damage in hyperuricemic mice were effectively recovered by CSF-E at 120 mg kg-1. CSF-E possessed anti-hyperuricemic and nephroprotective effects by suppressing XOD activity, improving renal function and regulating renal mURAT1 and mOAT1 protein expression, which resulted in beneficial effects on hyperuricemia and gout prevention.

Authors+Show Affiliations

School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China. zhenzhou.zhu@whpu.edu.cn jingren.he@whpu.edu.cn.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30327809

Citation

Zhang, Rui, et al. "Anti-hyperuricemic and Nephroprotective Effects of Extracts From Chaenomeles Sinensis (Thouin) Koehne in Hyperuricemic Mice." Food & Function, vol. 9, no. 11, 2018, pp. 5778-5790.
Zhang R, Zhan S, Li S, et al. Anti-hyperuricemic and nephroprotective effects of extracts from Chaenomeles sinensis (Thouin) Koehne in hyperuricemic mice. Food Funct. 2018;9(11):5778-5790.
Zhang, R., Zhan, S., Li, S., Zhu, Z., He, J., Lorenzo, J. M., & Barba, F. J. (2018). Anti-hyperuricemic and nephroprotective effects of extracts from Chaenomeles sinensis (Thouin) Koehne in hyperuricemic mice. Food & Function, 9(11), 5778-5790. https://doi.org/10.1039/c8fo01480a
Zhang R, et al. Anti-hyperuricemic and Nephroprotective Effects of Extracts From Chaenomeles Sinensis (Thouin) Koehne in Hyperuricemic Mice. Food Funct. 2018 Nov 14;9(11):5778-5790. PubMed PMID: 30327809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-hyperuricemic and nephroprotective effects of extracts from Chaenomeles sinensis (Thouin) Koehne in hyperuricemic mice. AU - Zhang,Rui, AU - Zhan,Shaoying, AU - Li,Shuyi, AU - Zhu,Zhenzhou, AU - He,Jingren, AU - Lorenzo,Jose M, AU - Barba,Francisco J, PY - 2018/10/18/pubmed PY - 2019/3/21/medline PY - 2018/10/18/entrez SP - 5778 EP - 5790 JF - Food & function JO - Food Funct VL - 9 IS - 11 N2 - Clinically, Chaenomeles sinensis (Thouin) Koehne (C. sinensis) has been used to treat hyperuricemia and gout. However, the exact mechanism of action is still unknown. In the present study, the ethyl acetate fraction of C. sinensis fruit extract (CSF-E) was separated. Potassium oxonate (PO)-induced hyperuricemic mice and normal mice were administered with CSF-E at 60, 120 and 180 mg kg-1, respectively for 7 days. Serum uric acid, creatinine and BUN levels, liver oxidative damage, and serum and hepatic XOD activities were primarily measured using assay kits. The evaluation of its nephroprotective effects was carried out by renal histopathological analysis. Simultaneously, renal protein levels of organic anion transporters, such as mURAT1 and mOAT1, were detected using western blotting to elucidate the possible mechanisms. The results showed that CSF-E could significantly inhibit XOD activities in both serum and liver (p < 0.05), decreasing uric acid, creatinine and BUN levels in serum, and increasing levels in the excretion of uric acid by down-regulated of mURAT1 and up-regulated mOAT1 protein expression of kidney in hyperuricemic mice. Moreover, PO-induced alterations in the levels of MDA, hepatic SOD and GSH-Px activities and renal inflammation damage in hyperuricemic mice were effectively recovered by CSF-E at 120 mg kg-1. CSF-E possessed anti-hyperuricemic and nephroprotective effects by suppressing XOD activity, improving renal function and regulating renal mURAT1 and mOAT1 protein expression, which resulted in beneficial effects on hyperuricemia and gout prevention. SN - 2042-650X UR - https://www.unboundmedicine.com/medline/citation/30327809/Anti_hyperuricemic_and_nephroprotective_effects_of_extracts_from_Chaenomeles_sinensis__Thouin__Koehne_in_hyperuricemic_mice_ L2 - https://doi.org/10.1039/c8fo01480a DB - PRIME DP - Unbound Medicine ER -