Tags

Type your tag names separated by a space and hit enter

Blockade of Endogenous Angiotensin-(1-7) in Hypothalamic Paraventricular Nucleus Attenuates High Salt-Induced Sympathoexcitation and Hypertension.
Neurosci Bull 2019; 35(1):47-56NB

Abstract

Angiotensin (Ang)-(1-7) is an important biologically-active peptide of the renin-angiotensin system. This study was designed to determine whether inhibition of Ang-(1-7) in the hypothalamic paraventricular nucleus (PVN) attenuates sympathetic activity and elevates blood pressure by modulating pro-inflammatory cytokines (PICs) and oxidative stress in the PVN in salt-induced hypertension. Rats were fed either a high-salt (8% NaCl) or a normal salt diet (0.3% NaCl) for 10 weeks, followed by bilateral microinjections of the Ang-(1-7) antagonist A-779 or vehicle into the PVN. We found that the mean arterial pressure (MAP), renal sympathetic nerve activity (RSNA), and plasma norepinephrine (NE) were significantly increased in salt-induced hypertensive rats. The high-salt diet also resulted in higher levels of the PICs interleukin-6, interleukin-1beta, tumor necrosis factor alpha, and monocyte chemotactic protein-1, as well as higher gp91phox expression and superoxide production in the PVN. Microinjection of A-779 (3 nmol/50 nL) into the bilateral PVN of hypertensive rats not only attenuated MAP, RSNA, and NE, but also decreased the PICs and oxidative stress in the PVN. These results suggest that the increased MAP and sympathetic activity in salt-induced hypertension can be suppressed by blockade of endogenous Ang-(1-7) in the PVN, through modulation of PICs and oxidative stress.

Authors+Show Affiliations

Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Sciences, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, 710061, China.Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Sciences, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, 710061, China. Genetic Engineering Laboratory, College of Biotechnology, Xi'an University, Xi'an, 710065, China.Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Sciences, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, 710061, China.Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Sciences, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, 710061, China.Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Sciences, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, 710061, China.Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Sciences, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, 710061, China.Department of Plastic and Cosmetic Surgery, Gansu Provincial Hospital, Lanzhou, 730000, China.Department of Pharmacology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, China.Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Sciences, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, 710061, China.Department of Cardiovascular Medicine, First Affiliated Hospital of the Medical College of Xi'an Jiaotong University, Xi'an, 710061, China.Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.Department of Endocrinology and Metabolism, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, China.Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Physiology, Nanjing Medical University, Nanjing, 210029, China.Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, 70803, USA.Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, 70803, USA. jfrancis@lsu.edu.Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Sciences, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, 710061, China. ykang@mail.xjtu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30328008

Citation

Yu, Xiao-Jing, et al. "Blockade of Endogenous Angiotensin-(1-7) in Hypothalamic Paraventricular Nucleus Attenuates High Salt-Induced Sympathoexcitation and Hypertension." Neuroscience Bulletin, vol. 35, no. 1, 2019, pp. 47-56.
Yu XJ, Miao YW, Li HB, et al. Blockade of Endogenous Angiotensin-(1-7) in Hypothalamic Paraventricular Nucleus Attenuates High Salt-Induced Sympathoexcitation and Hypertension. Neurosci Bull. 2019;35(1):47-56.
Yu, X. J., Miao, Y. W., Li, H. B., Su, Q., Liu, K. L., Fu, L. Y., ... Kang, Y. M. (2019). Blockade of Endogenous Angiotensin-(1-7) in Hypothalamic Paraventricular Nucleus Attenuates High Salt-Induced Sympathoexcitation and Hypertension. Neuroscience Bulletin, 35(1), pp. 47-56. doi:10.1007/s12264-018-0297-4.
Yu XJ, et al. Blockade of Endogenous Angiotensin-(1-7) in Hypothalamic Paraventricular Nucleus Attenuates High Salt-Induced Sympathoexcitation and Hypertension. Neurosci Bull. 2019;35(1):47-56. PubMed PMID: 30328008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Blockade of Endogenous Angiotensin-(1-7) in Hypothalamic Paraventricular Nucleus Attenuates High Salt-Induced Sympathoexcitation and Hypertension. AU - Yu,Xiao-Jing, AU - Miao,Yu-Wang, AU - Li,Hong-Bao, AU - Su,Qing, AU - Liu,Kai-Li, AU - Fu,Li-Yan, AU - Hou,Yi-Kang, AU - Shi,Xiao-Lian, AU - Li,Ying, AU - Mu,Jian-Jun, AU - Chen,Wen-Sheng, AU - Cui,Wei, AU - Zhu,Guo-Qing, AU - Ebenezer,Philip J, AU - Francis,Joseph, AU - Kang,Yu-Ming, Y1 - 2018/10/16/ PY - 2018/02/02/received PY - 2018/08/24/accepted PY - 2020/02/01/pmc-release PY - 2018/10/18/pubmed PY - 2019/4/10/medline PY - 2018/10/18/entrez KW - Angiotensin-(1–7) KW - High-salt diet KW - Hypertension KW - Paraventricular nucleus KW - Pro-inflammatory cytokines SP - 47 EP - 56 JF - Neuroscience bulletin JO - Neurosci Bull VL - 35 IS - 1 N2 - Angiotensin (Ang)-(1-7) is an important biologically-active peptide of the renin-angiotensin system. This study was designed to determine whether inhibition of Ang-(1-7) in the hypothalamic paraventricular nucleus (PVN) attenuates sympathetic activity and elevates blood pressure by modulating pro-inflammatory cytokines (PICs) and oxidative stress in the PVN in salt-induced hypertension. Rats were fed either a high-salt (8% NaCl) or a normal salt diet (0.3% NaCl) for 10 weeks, followed by bilateral microinjections of the Ang-(1-7) antagonist A-779 or vehicle into the PVN. We found that the mean arterial pressure (MAP), renal sympathetic nerve activity (RSNA), and plasma norepinephrine (NE) were significantly increased in salt-induced hypertensive rats. The high-salt diet also resulted in higher levels of the PICs interleukin-6, interleukin-1beta, tumor necrosis factor alpha, and monocyte chemotactic protein-1, as well as higher gp91phox expression and superoxide production in the PVN. Microinjection of A-779 (3 nmol/50 nL) into the bilateral PVN of hypertensive rats not only attenuated MAP, RSNA, and NE, but also decreased the PICs and oxidative stress in the PVN. These results suggest that the increased MAP and sympathetic activity in salt-induced hypertension can be suppressed by blockade of endogenous Ang-(1-7) in the PVN, through modulation of PICs and oxidative stress. SN - 1995-8218 UR - https://www.unboundmedicine.com/medline/citation/30328008/Blockade_of_Endogenous_Angiotensin__1_7__in_Hypothalamic_Paraventricular_Nucleus_Attenuates_High_Salt_Induced_Sympathoexcitation_and_Hypertension_ L2 - https://dx.doi.org/10.1007/s12264-018-0297-4 DB - PRIME DP - Unbound Medicine ER -