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Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model.
J Lipid Res. 2018 12; 59(12):2329-2338.JL

Abstract

The lipid matrix of the stratum corneum, the outermost skin layer, consists primarily of ceramides, cholesterol, and FFAs. These lipids form a trilayer long-periodicity phase (LPP) that is unique to this barrier. Knowledge about the LPP is essential in understanding the barrier function. Previous studies of LPP lipid models have identified the position of the major lipid classes and suggested that a large fraction of FFAs and the ceramide acyl chain are present in the central region. However, the precise arrangement, such as lipid subclass mixing (isolated or mixed) and ceramide conformation (extended or hairpin), remains unknown. Here, we deuterated FFAs and the ceramide acyl chain to study CD2 and CH2 interactions with Fourier-transform infrared spectroscopy. The ceramide and FFAs of various chain lengths were not in separate domains but had mixed together. The larger number of CD2-CD2 lipid chain interactions in the LPP than in a symmetrical bilayer structure implied that the ceramide had primarily adopted an extended conformation. Shorter FFAs were present in the central region of the LPP. This model explores the biophysical properties of the stratum corneum's LPP to improve the understanding of the barrier function of this layer.

Authors+Show Affiliations

Leiden Academic Centre for Drug Research, Division of BioTherapeutics, Gorlaeus Laboratories, University of Leiden, Leiden, The Netherlands.Leiden Academic Centre for Drug Research, Division of BioTherapeutics, Gorlaeus Laboratories, University of Leiden, Leiden, The Netherlands.Leiden Academic Centre for Drug Research, Division of BioTherapeutics, Gorlaeus Laboratories, University of Leiden, Leiden, The Netherlands bouwstra@lacdr.leidenuniv.nl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30333154

Citation

Beddoes, Charlotte M., et al. "Preferential Arrangement of Lipids in the Long-periodicity Phase of a Stratum Corneum Matrix Model." Journal of Lipid Research, vol. 59, no. 12, 2018, pp. 2329-2338.
Beddoes CM, Gooris GS, Bouwstra JA. Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model. J Lipid Res. 2018;59(12):2329-2338.
Beddoes, C. M., Gooris, G. S., & Bouwstra, J. A. (2018). Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model. Journal of Lipid Research, 59(12), 2329-2338. https://doi.org/10.1194/jlr.M087106
Beddoes CM, Gooris GS, Bouwstra JA. Preferential Arrangement of Lipids in the Long-periodicity Phase of a Stratum Corneum Matrix Model. J Lipid Res. 2018;59(12):2329-2338. PubMed PMID: 30333154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model. AU - Beddoes,Charlotte M, AU - Gooris,Gert S, AU - Bouwstra,Joke A, Y1 - 2018/10/17/ PY - 2018/05/18/received PY - 2018/09/28/revised PY - 2018/10/20/pubmed PY - 2019/9/3/medline PY - 2018/10/19/entrez KW - Fourier-transform infrared spectroscopy KW - ceramide KW - fatty acids KW - lipid conformation KW - membranes KW - partial deuteration KW - physical biochemistry KW - physical chemistry KW - skin KW - sphingolipids SP - 2329 EP - 2338 JF - Journal of lipid research JO - J Lipid Res VL - 59 IS - 12 N2 - The lipid matrix of the stratum corneum, the outermost skin layer, consists primarily of ceramides, cholesterol, and FFAs. These lipids form a trilayer long-periodicity phase (LPP) that is unique to this barrier. Knowledge about the LPP is essential in understanding the barrier function. Previous studies of LPP lipid models have identified the position of the major lipid classes and suggested that a large fraction of FFAs and the ceramide acyl chain are present in the central region. However, the precise arrangement, such as lipid subclass mixing (isolated or mixed) and ceramide conformation (extended or hairpin), remains unknown. Here, we deuterated FFAs and the ceramide acyl chain to study CD2 and CH2 interactions with Fourier-transform infrared spectroscopy. The ceramide and FFAs of various chain lengths were not in separate domains but had mixed together. The larger number of CD2-CD2 lipid chain interactions in the LPP than in a symmetrical bilayer structure implied that the ceramide had primarily adopted an extended conformation. Shorter FFAs were present in the central region of the LPP. This model explores the biophysical properties of the stratum corneum's LPP to improve the understanding of the barrier function of this layer. SN - 1539-7262 UR - https://www.unboundmedicine.com/medline/citation/30333154/Preferential_arrangement_of_lipids_in_the_long_periodicity_phase_of_a_stratum_corneum_matrix_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2275(20)34158-4 DB - PRIME DP - Unbound Medicine ER -