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[Mobilization of the pACYC184 plasmid by hybrid pAS8-121 delta plasmids in Escherichia coli cells].

Abstract

The plasmid pACYC184 is shown to be mobilized for conjugal transfer in Escherichia coli cells by the deleted (Tn7-TcR) derivatives of the hybrid conjugative plasmid pAS8-121 (RP4-Co1E1). Both the mobilized and mobilizing plasmids are autonomously inherited by the recipient cells when the mobilizing plasmid carries single copy of IS8 (the plasmid pAS8-121 delta 16). Cointegrates pAS8-121 delta 16D:: ::pACYC184 are found in the recipient cells with pACYC184 being inserted between two repeats of IS8 if the derivate plasmid pAS8-121 delta 16D having the duplication of IS8 is used to mobilize pACYC184 for conjugal transfer. The insertion of pACYC184 between IS8 repeats in the plasmid pAS8-121 delta 16D eliminates the plasmid ability to be inserted with high frequency into the chromosome of the phototrophic bacterium R. sphaeroides 2R. The cointegrate pAS8-121 delta 16D:: pACYC184 is stable but can be resolved during the transformation deriving the plasmid pACYC184:: IS8. The latter may be used as a probe for isolation and analysis of IS8 DNA sequences and for constructing the vectors on the basis of pACYC184.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

3033480

Citation

Muronets, E M., et al. "[Mobilization of the pACYC184 Plasmid By Hybrid pAS8-121 Delta Plasmids in Escherichia Coli Cells]." Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, 1987, pp. 12-6.
Muronets EM, Dubeĭkovskiĭ AN, Kameneva SV. [Mobilization of the pACYC184 plasmid by hybrid pAS8-121 delta plasmids in Escherichia coli cells]. Mol Gen Mikrobiol Virusol. 1987.
Muronets, E. M., Dubeĭkovskiĭ, A. N., & Kameneva, S. V. (1987). [Mobilization of the pACYC184 plasmid by hybrid pAS8-121 delta plasmids in Escherichia coli cells]. Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, (2), 12-6.
Muronets EM, Dubeĭkovskiĭ AN, Kameneva SV. [Mobilization of the pACYC184 Plasmid By Hybrid pAS8-121 Delta Plasmids in Escherichia Coli Cells]. Mol Gen Mikrobiol Virusol. 1987;(2)12-6. PubMed PMID: 3033480.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Mobilization of the pACYC184 plasmid by hybrid pAS8-121 delta plasmids in Escherichia coli cells]. AU - Muronets,E M, AU - Dubeĭkovskiĭ,A N, AU - Kameneva,S V, PY - 1987/2/1/pubmed PY - 1987/2/1/medline PY - 1987/2/1/entrez SP - 12 EP - 6 JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia JO - Mol. Gen. Mikrobiol. Virusol. IS - 2 N2 - The plasmid pACYC184 is shown to be mobilized for conjugal transfer in Escherichia coli cells by the deleted (Tn7-TcR) derivatives of the hybrid conjugative plasmid pAS8-121 (RP4-Co1E1). Both the mobilized and mobilizing plasmids are autonomously inherited by the recipient cells when the mobilizing plasmid carries single copy of IS8 (the plasmid pAS8-121 delta 16). Cointegrates pAS8-121 delta 16D:: ::pACYC184 are found in the recipient cells with pACYC184 being inserted between two repeats of IS8 if the derivate plasmid pAS8-121 delta 16D having the duplication of IS8 is used to mobilize pACYC184 for conjugal transfer. The insertion of pACYC184 between IS8 repeats in the plasmid pAS8-121 delta 16D eliminates the plasmid ability to be inserted with high frequency into the chromosome of the phototrophic bacterium R. sphaeroides 2R. The cointegrate pAS8-121 delta 16D:: pACYC184 is stable but can be resolved during the transformation deriving the plasmid pACYC184:: IS8. The latter may be used as a probe for isolation and analysis of IS8 DNA sequences and for constructing the vectors on the basis of pACYC184. SN - 0208-0613 UR - https://www.unboundmedicine.com/medline/citation/3033480/[Mobilization_of_the_pACYC184_plasmid_by_hybrid_pAS8_121_delta_plasmids_in_Escherichia_coli_cells]_ DB - PRIME DP - Unbound Medicine ER -