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Lowly expressed EGFR-AS1 promotes the progression of preeclampsia by inhibiting the EGFR-JAK/STAT signaling pathway.
Eur Rev Med Pharmacol Sci 2018; 22(19):6190-6197ER

Abstract

OBJECTIVE

To detect the differentially expressed long non-coding RNA (lncRNA) EGFR-AS1 in the placenta tissues of preeclampsia (PE) patients and normal pregnancies. We also investigated the underlying mechanism of EGFR-AS1 in regulating PE development.

PATIENTS AND METHODS

21 PE patients and 21 normal pregnancies admitted in our hospital were selected. The expression of EGFR Antisense RNA1 (EGFR-AS1) in the placenta tissues of PE patients and normal pregnancies was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). The regulatory effect of EGFR-AS1 on proliferation of HTR-8 cells was observed by Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Cell cycle and apoptosis of HTR-8 cells after overexpression or knockdown of EGFR-AS1 were detected by flow cytometry. The expression changes of EGFR and proteins related to JAK/STAT signaling pathway after knockdown of EGFR-AS1 in HTR-8 cells were detected by Western blot. Rescue experiments were carried out after upregulating EGFR expression in HTR-8 cells.

RESULTS

QRT-PCR results showed that the mRNA expression of EGFR-AS1 in PE patients was lower than that in normal pregnancies. CCK-8 assay and colony formation assay showed that knockdown of EGFR-AS1 in HTR-8 cells inhibited cell proliferation. Flow cytometry results showed that knockdown of EGFR-AS1 blocked the cell cycle of HTR-8 cells. Overexpression of EGFR-AS1 obtained opposite results. Western blot results showed that the protein expressions of EGFR, p-JAK and p-STAT were decreased after knockdown of EGFR-AS1. Alterations in protein expressions of p-JAK and p-STAT, and proliferative ability of HTR-8 cells induced by EGFR-AS1 knockdown could be rescued after upregulation of EGFR.

CONCLUSIONS

The expression of lncRNA EGFR-AS1 is decreased in PE patients, which can promote the progression of preeclampsia by inhibiting EGFR-JAK/STAT signaling pathway.

Authors+Show Affiliations

Department of Obstetrics, The Second People's Hospital of Liaocheng, Linqing, China. gtttut03733562@163.com.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30338781

Citation

Zhao, Z-M, and J Jiang. "Lowly Expressed EGFR-AS1 Promotes the Progression of Preeclampsia By Inhibiting the EGFR-JAK/STAT Signaling Pathway." European Review for Medical and Pharmacological Sciences, vol. 22, no. 19, 2018, pp. 6190-6197.
Zhao ZM, Jiang J. Lowly expressed EGFR-AS1 promotes the progression of preeclampsia by inhibiting the EGFR-JAK/STAT signaling pathway. Eur Rev Med Pharmacol Sci. 2018;22(19):6190-6197.
Zhao, Z. M., & Jiang, J. (2018). Lowly expressed EGFR-AS1 promotes the progression of preeclampsia by inhibiting the EGFR-JAK/STAT signaling pathway. European Review for Medical and Pharmacological Sciences, 22(19), pp. 6190-6197. doi:10.26355/eurrev_201810_16024.
Zhao ZM, Jiang J. Lowly Expressed EGFR-AS1 Promotes the Progression of Preeclampsia By Inhibiting the EGFR-JAK/STAT Signaling Pathway. Eur Rev Med Pharmacol Sci. 2018;22(19):6190-6197. PubMed PMID: 30338781.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lowly expressed EGFR-AS1 promotes the progression of preeclampsia by inhibiting the EGFR-JAK/STAT signaling pathway. AU - Zhao,Z-M, AU - Jiang,J, PY - 2018/10/20/entrez PY - 2018/10/20/pubmed PY - 2018/10/20/medline SP - 6190 EP - 6197 JF - European review for medical and pharmacological sciences JO - Eur Rev Med Pharmacol Sci VL - 22 IS - 19 N2 - OBJECTIVE: To detect the differentially expressed long non-coding RNA (lncRNA) EGFR-AS1 in the placenta tissues of preeclampsia (PE) patients and normal pregnancies. We also investigated the underlying mechanism of EGFR-AS1 in regulating PE development. PATIENTS AND METHODS: 21 PE patients and 21 normal pregnancies admitted in our hospital were selected. The expression of EGFR Antisense RNA1 (EGFR-AS1) in the placenta tissues of PE patients and normal pregnancies was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). The regulatory effect of EGFR-AS1 on proliferation of HTR-8 cells was observed by Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Cell cycle and apoptosis of HTR-8 cells after overexpression or knockdown of EGFR-AS1 were detected by flow cytometry. The expression changes of EGFR and proteins related to JAK/STAT signaling pathway after knockdown of EGFR-AS1 in HTR-8 cells were detected by Western blot. Rescue experiments were carried out after upregulating EGFR expression in HTR-8 cells. RESULTS: QRT-PCR results showed that the mRNA expression of EGFR-AS1 in PE patients was lower than that in normal pregnancies. CCK-8 assay and colony formation assay showed that knockdown of EGFR-AS1 in HTR-8 cells inhibited cell proliferation. Flow cytometry results showed that knockdown of EGFR-AS1 blocked the cell cycle of HTR-8 cells. Overexpression of EGFR-AS1 obtained opposite results. Western blot results showed that the protein expressions of EGFR, p-JAK and p-STAT were decreased after knockdown of EGFR-AS1. Alterations in protein expressions of p-JAK and p-STAT, and proliferative ability of HTR-8 cells induced by EGFR-AS1 knockdown could be rescued after upregulation of EGFR. CONCLUSIONS: The expression of lncRNA EGFR-AS1 is decreased in PE patients, which can promote the progression of preeclampsia by inhibiting EGFR-JAK/STAT signaling pathway. SN - 2284-0729 UR - https://www.unboundmedicine.com/medline/citation/30338781/Lowly_expressed_EGFR_AS1_promotes_the_progression_of_preeclampsia_by_inhibiting_the_EGFR_JAK/STAT_signaling_pathway_ L2 - https://www.europeanreview.org/article/16024 DB - PRIME DP - Unbound Medicine ER -
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