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Low expression of microRNA-21 inhibits trophoblast cell infiltration through targeting PTEN.

Abstract

OBJECTIVE

We investigate whether microRNA-21 could increase the infiltration ability of trophoblast cells via regulating PTEN expression, thus participating in the occurrence and development of preeclampsia.

PATIENTS AND METHODS

MicroRNA-21 expression in the placenta tissues of preeclampsia women and normal pregnant women was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The effects of microRNA-21 on cell proliferation and infiltration were examined by cell counting kit-8 (CCK-8) and transwell assay, respectively. The dual-luciferase reporter gene assay was used to determine the binding relationship between microRNA-21 and PTEN. Western blot was performed to detect PTEN and microRNA-21 in trophoblasts.

RESULTS

QRT-PCR results showed that the microRNA-21 expression was significantly lower in the placenta of the preeclampsia women than those of normal pregnant women. Overexpression of microRNA-21 in HTR-8/SVneo cells had no effect on cell proliferation, but enhanced cell infiltration ability. Inhibition of microRNA-21 in trophoblasts showed the opposite effects. The results of luciferase activity assay and Western blot showed that microRNA-21 could target PTEN and downregulate its expression. Overexpression of PTEN in HTR-8/SVneo cells partially reversed the enhanced invasive ability induced by microRNA-21 overexpression.

CONCLUSIONS

Low expression of microRNA-21 attenuated cell infiltration of trophoblasts via direct regulating PTEN expression.

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  • Authors+Show Affiliations

    ,

    Department of Gynecology and Obstetrics, The Third Hospital of Ji'nan, Ji'nan, China. Louchunx73@163.com.

    , , ,

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30338782

    Citation

    Lou, C-X, et al. "Low Expression of microRNA-21 Inhibits Trophoblast Cell Infiltration Through Targeting PTEN." European Review for Medical and Pharmacological Sciences, vol. 22, no. 19, 2018, pp. 6181-6189.
    Lou CX, Zhou XT, Tian QC, et al. Low expression of microRNA-21 inhibits trophoblast cell infiltration through targeting PTEN. Eur Rev Med Pharmacol Sci. 2018;22(19):6181-6189.
    Lou, C. X., Zhou, X. T., Tian, Q. C., Xie, H. Q., & Zhang, J. Y. (2018). Low expression of microRNA-21 inhibits trophoblast cell infiltration through targeting PTEN. European Review for Medical and Pharmacological Sciences, 22(19), pp. 6181-6189. doi:10.26355/eurrev_201810_16023.
    Lou CX, et al. Low Expression of microRNA-21 Inhibits Trophoblast Cell Infiltration Through Targeting PTEN. Eur Rev Med Pharmacol Sci. 2018;22(19):6181-6189. PubMed PMID: 30338782.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Low expression of microRNA-21 inhibits trophoblast cell infiltration through targeting PTEN. AU - Lou,C-X, AU - Zhou,X-T, AU - Tian,Q-C, AU - Xie,H-Q, AU - Zhang,J-Y, PY - 2018/10/20/entrez PY - 2018/10/20/pubmed PY - 2018/10/20/medline SP - 6181 EP - 6189 JF - European review for medical and pharmacological sciences JO - Eur Rev Med Pharmacol Sci VL - 22 IS - 19 N2 - OBJECTIVE: We investigate whether microRNA-21 could increase the infiltration ability of trophoblast cells via regulating PTEN expression, thus participating in the occurrence and development of preeclampsia. PATIENTS AND METHODS: MicroRNA-21 expression in the placenta tissues of preeclampsia women and normal pregnant women was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The effects of microRNA-21 on cell proliferation and infiltration were examined by cell counting kit-8 (CCK-8) and transwell assay, respectively. The dual-luciferase reporter gene assay was used to determine the binding relationship between microRNA-21 and PTEN. Western blot was performed to detect PTEN and microRNA-21 in trophoblasts. RESULTS: QRT-PCR results showed that the microRNA-21 expression was significantly lower in the placenta of the preeclampsia women than those of normal pregnant women. Overexpression of microRNA-21 in HTR-8/SVneo cells had no effect on cell proliferation, but enhanced cell infiltration ability. Inhibition of microRNA-21 in trophoblasts showed the opposite effects. The results of luciferase activity assay and Western blot showed that microRNA-21 could target PTEN and downregulate its expression. Overexpression of PTEN in HTR-8/SVneo cells partially reversed the enhanced invasive ability induced by microRNA-21 overexpression. CONCLUSIONS: Low expression of microRNA-21 attenuated cell infiltration of trophoblasts via direct regulating PTEN expression. SN - 2284-0729 UR - https://www.unboundmedicine.com/medline/citation/30338782/Low_expression_of_microRNA_21_inhibits_trophoblast_cell_infiltration_through_targeting_PTEN_ L2 - https://www.europeanreview.org/article/16023 DB - PRIME DP - Unbound Medicine ER -