Tags

Type your tag names separated by a space and hit enter

MicroRNA-200a promotes proliferation and invasion of ovarian cancer cells by targeting PTEN.
Eur Rev Med Pharmacol Sci 2018; 22(19):6260-6267ER

Abstract

OBJECTIVE

We investigate whether microRNA-200a could regulate proliferation and invasion of ovarian cancer cells, thereby participating in the occurrence and development of ovarian cancer. We also explore the specific mechanism of microRNA-200a in regulating ovarian cancer.

PATIENTS AND METHODS

Expression level of microRNA-200a in ovarian cancer tissues and paracancerous tissues were detected by quantitative Real-time polymerase chain reaction (qRT-PCR). The regulatory effects of microRNA-200a on proliferation and invasion of ovarian cancer cells were examined by Cell counting kit-8 (CCK-8) and cell invasion assay, respectively. Dual-luciferase reporter gene assay was performed to confirm the binding relationship between microRNA-200a and PTEN (phosphatase and tensin homolog deleted on chromosome ten). The regulatory role of microRNA-200a in PTEN expression was accessed by Western blot. Rescue experiments were conducted to assess whether microRNA-200a regulated proliferation and invasion of ovarian cancer cells by inhibiting PTEN expression.

RESULTS

MicroRNA-200a expression in ovarian cancer tissues was significantly higher than that of paracancerous tissues. Besides, microRNA-200a was also overexpressed in ovarian cancer cell lines than that of normal ovarian cells. Overexpression of microRNA-200a promoted the proliferative and invasive abilities of SKOV3 and OVCAR3 cells. Dual-luciferase reporter gene assay showed that microRNA-200a could directly degrade PTEN. Overexpression of PTEN in SKOV3 and OVCAR3 cells partially reversed the increased cell proliferation and invasion induced by overexpressed microRNA-200a.

CONCLUSIONS

Overexpressed microRNA-200a promoted the proliferative and invasive abilities of ovarian cancer cells, which might be related to the targeted regulation of PTEN expression.

Authors+Show Affiliations

Department of Gynecology, Zhongnan Hospital of Wuhan University, Wuhan, China. zw6676@163.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30338796

Citation

Jiang, J-H, et al. "MicroRNA-200a Promotes Proliferation and Invasion of Ovarian Cancer Cells By Targeting PTEN." European Review for Medical and Pharmacological Sciences, vol. 22, no. 19, 2018, pp. 6260-6267.
Jiang JH, Lv QY, Yi YX, et al. MicroRNA-200a promotes proliferation and invasion of ovarian cancer cells by targeting PTEN. Eur Rev Med Pharmacol Sci. 2018;22(19):6260-6267.
Jiang, J. H., Lv, Q. Y., Yi, Y. X., Liao, J., Wang, X. W., & Zhang, W. (2018). MicroRNA-200a promotes proliferation and invasion of ovarian cancer cells by targeting PTEN. European Review for Medical and Pharmacological Sciences, 22(19), pp. 6260-6267. doi:10.26355/eurrev_201810_16033.
Jiang JH, et al. MicroRNA-200a Promotes Proliferation and Invasion of Ovarian Cancer Cells By Targeting PTEN. Eur Rev Med Pharmacol Sci. 2018;22(19):6260-6267. PubMed PMID: 30338796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MicroRNA-200a promotes proliferation and invasion of ovarian cancer cells by targeting PTEN. AU - Jiang,J-H, AU - Lv,Q-Y, AU - Yi,Y-X, AU - Liao,J, AU - Wang,X-W, AU - Zhang,W, PY - 2018/10/20/entrez PY - 2018/10/20/pubmed PY - 2018/10/20/medline SP - 6260 EP - 6267 JF - European review for medical and pharmacological sciences JO - Eur Rev Med Pharmacol Sci VL - 22 IS - 19 N2 - OBJECTIVE: We investigate whether microRNA-200a could regulate proliferation and invasion of ovarian cancer cells, thereby participating in the occurrence and development of ovarian cancer. We also explore the specific mechanism of microRNA-200a in regulating ovarian cancer. PATIENTS AND METHODS: Expression level of microRNA-200a in ovarian cancer tissues and paracancerous tissues were detected by quantitative Real-time polymerase chain reaction (qRT-PCR). The regulatory effects of microRNA-200a on proliferation and invasion of ovarian cancer cells were examined by Cell counting kit-8 (CCK-8) and cell invasion assay, respectively. Dual-luciferase reporter gene assay was performed to confirm the binding relationship between microRNA-200a and PTEN (phosphatase and tensin homolog deleted on chromosome ten). The regulatory role of microRNA-200a in PTEN expression was accessed by Western blot. Rescue experiments were conducted to assess whether microRNA-200a regulated proliferation and invasion of ovarian cancer cells by inhibiting PTEN expression. RESULTS: MicroRNA-200a expression in ovarian cancer tissues was significantly higher than that of paracancerous tissues. Besides, microRNA-200a was also overexpressed in ovarian cancer cell lines than that of normal ovarian cells. Overexpression of microRNA-200a promoted the proliferative and invasive abilities of SKOV3 and OVCAR3 cells. Dual-luciferase reporter gene assay showed that microRNA-200a could directly degrade PTEN. Overexpression of PTEN in SKOV3 and OVCAR3 cells partially reversed the increased cell proliferation and invasion induced by overexpressed microRNA-200a. CONCLUSIONS: Overexpressed microRNA-200a promoted the proliferative and invasive abilities of ovarian cancer cells, which might be related to the targeted regulation of PTEN expression. SN - 2284-0729 UR - https://www.unboundmedicine.com/medline/citation/30338796/MicroRNA_200a_promotes_proliferation_and_invasion_of_ovarian_cancer_cells_by_targeting_PTEN_ L2 - https://www.europeanreview.org/article/16033 DB - PRIME DP - Unbound Medicine ER -