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MicroRNA-203 inhibits invasion and induces apoptosis of laryngeal cancer cells via targeting LASP1.

Abstract

OBJECTIVE

To explore the role of microRNA-203 in laryngeal cancer and its underlying mechanism in regulating cell invasion and apoptosis.

PATIENTS AND METHODS

MicroRNA-203 expression in laryngeal cancer tissues and paracancerous tissues was detected by quantitative real time-polymerase chain reaction (qRT-PCR). The regulatory effects of microRNA-203 on the invasion and apoptosis of laryngeal cancer cells were detected by transwell assay and flow cytometry, respectively. Dual-Luciferase reporter gene assay was performed to access the binding condition of microRNA-203 and LASP1. Both mRNA and protein levels of LASP1 in laryngeal cancer cells were detected after transfection with microRNA-203 mimic or microRNA-203 inhibitor by qRT-PCR and Western blot, respectively. Rescue experiments were finally performed to detect whether microRNA-203 regulates laryngeal cancer development via targeting LASP1.

RESULTS

MicroRNA-203 was lowly expressed in laryngeal cancer tissues and cell lines. MicroRNA-203 knockdown in Hep-2 cells can promote the invasion and inhibit the apoptosis of laryngeal cancer cells. Subsequently, LASP1 was predicted to be the target gene of microRNA-203, which was further verified by the Dual-Luciferase reporter gene assay. LASP1 expression was negatively regulated by microRNA-203. Furthermore, rescue experiments showed that the regulatory effects of microRNA-203 on the invasion and apoptosis of laryngeal cancer cells were reversed by LASP1.

CONCLUSIONS

We showed that lowly expressed microRNA-203 could promote the invasion and inhibit apoptosis of laryngeal cancer cells via inhibiting LASP1.

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  • Authors+Show Affiliations

    ,

    Department of Otorhinolaryngology and Head and Neck Surgery, Peking University People's Hospital, Beijing, China. yulish68@163.com.

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    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30338803

    Citation

    Tan, J, et al. "MicroRNA-203 Inhibits Invasion and Induces Apoptosis of Laryngeal Cancer Cells Via Targeting LASP1." European Review for Medical and Pharmacological Sciences, vol. 22, no. 19, 2018, pp. 6350-6357.
    Tan J, Jing YY, Han L, et al. MicroRNA-203 inhibits invasion and induces apoptosis of laryngeal cancer cells via targeting LASP1. Eur Rev Med Pharmacol Sci. 2018;22(19):6350-6357.
    Tan, J., Jing, Y. Y., Han, L., Zheng, H. W., Shen, J. X., Zhang, L. H., & Yu, L. S. (2018). MicroRNA-203 inhibits invasion and induces apoptosis of laryngeal cancer cells via targeting LASP1. European Review for Medical and Pharmacological Sciences, 22(19), pp. 6350-6357. doi:10.26355/eurrev_201810_16046.
    Tan J, et al. MicroRNA-203 Inhibits Invasion and Induces Apoptosis of Laryngeal Cancer Cells Via Targeting LASP1. Eur Rev Med Pharmacol Sci. 2018;22(19):6350-6357. PubMed PMID: 30338803.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - MicroRNA-203 inhibits invasion and induces apoptosis of laryngeal cancer cells via targeting LASP1. AU - Tan,J, AU - Jing,Y-Y, AU - Han,L, AU - Zheng,H-W, AU - Shen,J-X, AU - Zhang,L-H, AU - Yu,L-S, PY - 2018/10/20/entrez PY - 2018/10/20/pubmed PY - 2018/10/20/medline SP - 6350 EP - 6357 JF - European review for medical and pharmacological sciences JO - Eur Rev Med Pharmacol Sci VL - 22 IS - 19 N2 - OBJECTIVE: To explore the role of microRNA-203 in laryngeal cancer and its underlying mechanism in regulating cell invasion and apoptosis. PATIENTS AND METHODS: MicroRNA-203 expression in laryngeal cancer tissues and paracancerous tissues was detected by quantitative real time-polymerase chain reaction (qRT-PCR). The regulatory effects of microRNA-203 on the invasion and apoptosis of laryngeal cancer cells were detected by transwell assay and flow cytometry, respectively. Dual-Luciferase reporter gene assay was performed to access the binding condition of microRNA-203 and LASP1. Both mRNA and protein levels of LASP1 in laryngeal cancer cells were detected after transfection with microRNA-203 mimic or microRNA-203 inhibitor by qRT-PCR and Western blot, respectively. Rescue experiments were finally performed to detect whether microRNA-203 regulates laryngeal cancer development via targeting LASP1. RESULTS: MicroRNA-203 was lowly expressed in laryngeal cancer tissues and cell lines. MicroRNA-203 knockdown in Hep-2 cells can promote the invasion and inhibit the apoptosis of laryngeal cancer cells. Subsequently, LASP1 was predicted to be the target gene of microRNA-203, which was further verified by the Dual-Luciferase reporter gene assay. LASP1 expression was negatively regulated by microRNA-203. Furthermore, rescue experiments showed that the regulatory effects of microRNA-203 on the invasion and apoptosis of laryngeal cancer cells were reversed by LASP1. CONCLUSIONS: We showed that lowly expressed microRNA-203 could promote the invasion and inhibit apoptosis of laryngeal cancer cells via inhibiting LASP1. SN - 2284-0729 UR - https://www.unboundmedicine.com/medline/citation/30338803/MicroRNA_203_inhibits_invasion_and_induces_apoptosis_of_laryngeal_cancer_cells_via_targeting_LASP1_ L2 - https://www.europeanreview.org/article/16046 DB - PRIME DP - Unbound Medicine ER -