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Fish Skin Gelatin Hydrolysate Production by Ginger Powder Induces Glutathione Synthesis To Prevent Hydrogen Peroxide Induced Intestinal Oxidative Stress via the Pept1-p62-Nrf2 Cascade.
J Agric Food Chem. 2018 Nov 07; 66(44):11601-11611.JA

Abstract

Gelatin hydrolysate was reported to contain large amounts of biologically active peptides with excellent antioxidant properties. However, its inducement of antioxidant response within cells and the underlying molecular mechanism are far from clear. Here, gelatin from Nile tilapia skin was hydrolyzed by ginger protease to produce antioxidant hydrolysate, and three fish skin gelatin hydrolysate fractions (FSGHFs) were obtained by ultrafiltration. Fractionation of the hydrolysate increased the free radical scavenging capacity of the FSGHFs, particularly FSGHF3, which showed the lowest molecular weight (below 1000 Da). Furthermore, FSGHF3 treatment prior to H2O2 exposure increased cell viability and membrane integrity in IPEC-J2 cells. H2O2-induced ROS production and epithelial barrier damage were suppressed by FSGHF3 pretreatment. FSGHF3 stimulated the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2), along with increases in the mRNA and protein expression of catalytic and modulatory subunits of γ-glutamylcysteine ligase as well as in the level of glutathione. Silencing of Nrf2 or p62 (an upstream regulator of Nrf2) suppressed FSGHF3-induced Nrf2 activation and its protection against H2O2-induced oxidative stress. Moreover, oligopeptides in FSGHF3 may mediate the cytoprotective effect against oxidative stress, which was confirmed by the result that FSGHF3 failed to inhibit the ROS production in H2O2-exposed cells with the knockdown of Pept1 (an oligopeptide transporter). Therefore, FSGHF3 can induce glutathione synthesis and prevent oxidative stress through the Pept1-p62-Nrf2 cascade and thus may be a functional food for gastrointestinal dysfunction.

Authors+Show Affiliations

Department of Animal Nutrition and Feed Science, College of Animal Science and Technology , Huazhong Agricultural University , Wuhan 430070 , People's Republic of China. State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang 330047 , People's Republic of China.Department of Animal Nutrition and Feed Science, College of Animal Science and Technology , Huazhong Agricultural University , Wuhan 430070 , People's Republic of China. The Cooperative Innovation Center for Sustainable Pig Production , Wuhan 430070 , People's Republic of China.Department of Animal Nutrition and Feed Science, College of Animal Science and Technology , Huazhong Agricultural University , Wuhan 430070 , People's Republic of China.State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang 330047 , People's Republic of China.Department of Animal Nutrition and Feed Science, College of Animal Science and Technology , Huazhong Agricultural University , Wuhan 430070 , People's Republic of China.Department of Animal Nutrition and Feed Science, College of Animal Science and Technology , Huazhong Agricultural University , Wuhan 430070 , People's Republic of China. The Cooperative Innovation Center for Sustainable Pig Production , Wuhan 430070 , People's Republic of China.Department of Animal Nutrition and Feed Science, College of Animal Science and Technology , Huazhong Agricultural University , Wuhan 430070 , People's Republic of China. The Cooperative Innovation Center for Sustainable Pig Production , Wuhan 430070 , People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30339007

Citation

Zheng, Liufeng, et al. "Fish Skin Gelatin Hydrolysate Production By Ginger Powder Induces Glutathione Synthesis to Prevent Hydrogen Peroxide Induced Intestinal Oxidative Stress Via the Pept1-p62-Nrf2 Cascade." Journal of Agricultural and Food Chemistry, vol. 66, no. 44, 2018, pp. 11601-11611.
Zheng L, Wei H, Yu H, et al. Fish Skin Gelatin Hydrolysate Production by Ginger Powder Induces Glutathione Synthesis To Prevent Hydrogen Peroxide Induced Intestinal Oxidative Stress via the Pept1-p62-Nrf2 Cascade. J Agric Food Chem. 2018;66(44):11601-11611.
Zheng, L., Wei, H., Yu, H., Xing, Q., Zou, Y., Zhou, Y., & Peng, J. (2018). Fish Skin Gelatin Hydrolysate Production by Ginger Powder Induces Glutathione Synthesis To Prevent Hydrogen Peroxide Induced Intestinal Oxidative Stress via the Pept1-p62-Nrf2 Cascade. Journal of Agricultural and Food Chemistry, 66(44), 11601-11611. https://doi.org/10.1021/acs.jafc.8b02840
Zheng L, et al. Fish Skin Gelatin Hydrolysate Production By Ginger Powder Induces Glutathione Synthesis to Prevent Hydrogen Peroxide Induced Intestinal Oxidative Stress Via the Pept1-p62-Nrf2 Cascade. J Agric Food Chem. 2018 Nov 7;66(44):11601-11611. PubMed PMID: 30339007.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fish Skin Gelatin Hydrolysate Production by Ginger Powder Induces Glutathione Synthesis To Prevent Hydrogen Peroxide Induced Intestinal Oxidative Stress via the Pept1-p62-Nrf2 Cascade. AU - Zheng,Liufeng, AU - Wei,Hongkui, AU - Yu,Huichao, AU - Xing,Qian, AU - Zou,Yi, AU - Zhou,Yuanfei, AU - Peng,Jian, Y1 - 2018/10/30/ PY - 2018/10/20/pubmed PY - 2018/11/16/medline PY - 2018/10/20/entrez KW - Pept1-p62-Nrf2 cascade KW - fish skin gelatin hydrolysate KW - glutathione synthesis KW - oxidative stress KW - small intestinal epithelial cells SP - 11601 EP - 11611 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 66 IS - 44 N2 - Gelatin hydrolysate was reported to contain large amounts of biologically active peptides with excellent antioxidant properties. However, its inducement of antioxidant response within cells and the underlying molecular mechanism are far from clear. Here, gelatin from Nile tilapia skin was hydrolyzed by ginger protease to produce antioxidant hydrolysate, and three fish skin gelatin hydrolysate fractions (FSGHFs) were obtained by ultrafiltration. Fractionation of the hydrolysate increased the free radical scavenging capacity of the FSGHFs, particularly FSGHF3, which showed the lowest molecular weight (below 1000 Da). Furthermore, FSGHF3 treatment prior to H2O2 exposure increased cell viability and membrane integrity in IPEC-J2 cells. H2O2-induced ROS production and epithelial barrier damage were suppressed by FSGHF3 pretreatment. FSGHF3 stimulated the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2), along with increases in the mRNA and protein expression of catalytic and modulatory subunits of γ-glutamylcysteine ligase as well as in the level of glutathione. Silencing of Nrf2 or p62 (an upstream regulator of Nrf2) suppressed FSGHF3-induced Nrf2 activation and its protection against H2O2-induced oxidative stress. Moreover, oligopeptides in FSGHF3 may mediate the cytoprotective effect against oxidative stress, which was confirmed by the result that FSGHF3 failed to inhibit the ROS production in H2O2-exposed cells with the knockdown of Pept1 (an oligopeptide transporter). Therefore, FSGHF3 can induce glutathione synthesis and prevent oxidative stress through the Pept1-p62-Nrf2 cascade and thus may be a functional food for gastrointestinal dysfunction. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/30339007/Fish_Skin_Gelatin_Hydrolysate_Production_by_Ginger_Powder_Induces_Glutathione_Synthesis_To_Prevent_Hydrogen_Peroxide_Induced_Intestinal_Oxidative_Stress_via_the_Pept1_p62_Nrf2_Cascade_ L2 - https://doi.org/10.1021/acs.jafc.8b02840 DB - PRIME DP - Unbound Medicine ER -