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Biomedical potential of chitosan-silver nanoparticles with special reference to antioxidant, antibacterial, hemolytic and in vivo cutaneous wound healing effects.
Biochim Biophys Acta Gen Subj. 2019 01; 1863(1):241-254.BB

Abstract

The aim of the present study was to prepare chitosan-PVA-silver nanoparticles (CS-AgNPs) through green method. Chitosan and PVA polymers acted as stabilizing agents. DLS and TEM analyses showed that CS-AgNPs were homogeneously dispersed in matrix with an average size of 190-200 nm. The CS-AgNPs were tested for their antioxidant and antibacterial properties and the results revealed that they exhibited higher antioxidant activity than CS powder. Moreover, CS-AgNPs were characterized by a low cytotoxicity effect at 5-200 μg/ml against Chinese Hamster Ovary (CHO-K1) cells. In addition, the prepared CS-Ag NPs were found to promote significantly the wound healing, as determined by the wound contraction ratio and histological examination. A significant improvement in wound healing progression and in oxidative stress damage were observed for CS, CS-PVA and CS-AgNPs-treated wound tissues, when compared to control and CICAFLORA®-treated groups. The wound healing effect could be attributed to the antibacterial and antioxidant synergy of AgNPs and CS. Results strongly support the possibility of using CS-AgNPs for wound care applications.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Hajji S
Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. 1173, 3038 Sfax, Tunisia. Electronic address: hajjisawssen@yahoo.fr.
Khedir SB
Laboratory of Histology and Embryology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.
Hamza-Mnif I
Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. 1173, 3038 Sfax, Tunisia.
Hamdi M
Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. 1173, 3038 Sfax, Tunisia.
Jedidi I
Laboratory of Hematology, CHU Habib Bourguiba, University Hospital, Sfax, Tunisia.
Kallel R
Laboratory of Pathological Anatomy and Cytology, CHU Habib Bourguiba, University Hospital, Sfax, Tunisia.
Boufi S
LMSE, University of Sfax, Faculty of Science of Sfax, Tunisia.
Nasri M
Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. 1173, 3038 Sfax, Tunisia.

MeSH

AnimalsAnti-Bacterial AgentsAntioxidantsBiphenyl CompoundsCHO CellsChitosanCricetinaeCricetulusEscherichia coliFree Radical ScavengersFree RadicalsGelsHemolysisHumansIronLinoleic AcidMaleMetal NanoparticlesMicrobial Sensitivity TestsOxidative StressOxygenPicratesRatsRats, WistarSilverSilver NitrateSkinStaphylococcus aureusWound Healingbeta Carotene

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30339915

Citation

Hajji, Sawssen, et al. "Biomedical Potential of Chitosan-silver Nanoparticles With Special Reference to Antioxidant, Antibacterial, Hemolytic and in Vivo Cutaneous Wound Healing Effects." Biochimica Et Biophysica Acta. General Subjects, vol. 1863, no. 1, 2019, pp. 241-254.
Hajji S, Khedir SB, Hamza-Mnif I, et al. Biomedical potential of chitosan-silver nanoparticles with special reference to antioxidant, antibacterial, hemolytic and in vivo cutaneous wound healing effects. Biochim Biophys Acta Gen Subj. 2019;1863(1):241-254.
Hajji, S., Khedir, S. B., Hamza-Mnif, I., Hamdi, M., Jedidi, I., Kallel, R., Boufi, S., & Nasri, M. (2019). Biomedical potential of chitosan-silver nanoparticles with special reference to antioxidant, antibacterial, hemolytic and in vivo cutaneous wound healing effects. Biochimica Et Biophysica Acta. General Subjects, 1863(1), 241-254. https://doi.org/10.1016/j.bbagen.2018.10.010
Hajji S, et al. Biomedical Potential of Chitosan-silver Nanoparticles With Special Reference to Antioxidant, Antibacterial, Hemolytic and in Vivo Cutaneous Wound Healing Effects. Biochim Biophys Acta Gen Subj. 2019;1863(1):241-254. PubMed PMID: 30339915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biomedical potential of chitosan-silver nanoparticles with special reference to antioxidant, antibacterial, hemolytic and in vivo cutaneous wound healing effects. AU - Hajji,Sawssen, AU - Khedir,Sameh Ben, AU - Hamza-Mnif,Ibtissem, AU - Hamdi,Marwa, AU - Jedidi,Ines, AU - Kallel,Rim, AU - Boufi,Sami, AU - Nasri,Moncef, Y1 - 2018/10/16/ PY - 2018/06/27/received PY - 2018/10/03/revised PY - 2018/10/13/accepted PY - 2018/10/20/pubmed PY - 2019/8/17/medline PY - 2018/10/20/entrez KW - Antioxidant and antibacterial activities KW - Chitosan-silver nanoparticles KW - Cytotoxicity effect KW - Structural characterization KW - Wound-healing efficiency SP - 241 EP - 254 JF - Biochimica et biophysica acta. General subjects JO - Biochim Biophys Acta Gen Subj VL - 1863 IS - 1 N2 - The aim of the present study was to prepare chitosan-PVA-silver nanoparticles (CS-AgNPs) through green method. Chitosan and PVA polymers acted as stabilizing agents. DLS and TEM analyses showed that CS-AgNPs were homogeneously dispersed in matrix with an average size of 190-200 nm. The CS-AgNPs were tested for their antioxidant and antibacterial properties and the results revealed that they exhibited higher antioxidant activity than CS powder. Moreover, CS-AgNPs were characterized by a low cytotoxicity effect at 5-200 μg/ml against Chinese Hamster Ovary (CHO-K1) cells. In addition, the prepared CS-Ag NPs were found to promote significantly the wound healing, as determined by the wound contraction ratio and histological examination. A significant improvement in wound healing progression and in oxidative stress damage were observed for CS, CS-PVA and CS-AgNPs-treated wound tissues, when compared to control and CICAFLORA®-treated groups. The wound healing effect could be attributed to the antibacterial and antioxidant synergy of AgNPs and CS. Results strongly support the possibility of using CS-AgNPs for wound care applications. SN - 1872-8006 UR - https://www.unboundmedicine.com/medline/citation/30339915/Biomedical_potential_of_chitosan_silver_nanoparticles_with_special_reference_to_antioxidant_antibacterial_hemolytic_and_in_vivo_cutaneous_wound_healing_effects_ DB - PRIME DP - Unbound Medicine ER -
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