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Influence of pristine and hydrophobic ZnO nanoparticles on cytotoxicity and endoplasmic reticulum (ER) stress-autophagy-apoptosis gene expression in A549-macrophage co-culture.
Ecotoxicol Environ Saf. 2019 Jan 15; 167:188-195.EE

Abstract

Exposure to ZnO nanoparticles (NPs) might modulate endoplasmic reticulum (ER) stress-autophagy gene expression, but the possible influence of hydrophobic surface coating on these responses was less studied. This study used A549-macrophage co-culture as the in vitro model for lung barrier and investigated the toxicity of pristine and hydrophobic ZnO NPs. Pristine and hydrophobic NPs exhibited different Zeta potential and solubility in water, which suggested that hydrophobic surface coating might alter the colloidal aspects of ZnO NPs. However, pristine and hydrophobic ZnO NPs induced cytotoxicity and reduced the release of soluble monocyte chemotactic protein-1 (sMCP-1) in A549-macrophage co-culture to a similar extent. Exposure to pristine ZnO NPs significantly promoted the expression of ER stress-apoptosis genes, namely DDIT3, XBP-1s, CASP9, CASP12 and BAX (p < 0.05), but hydrophobic ZnO NPs only significantly promoted the expression of BAX (p < 0.05). Exposure to pristine ZnO NPs also significantly reduced the expression of autophagic gene BECN1 (p < 0.05) but not ATG7 (p > 0.05), whereas hydrophobic ZnO NPs significantly reduced the expression of ATG7 and BECN1 (p < 0.01). Moreover, the expression of XBP-1s, HSPA5, CASP9, CASP12, BAX and ATG7 in pristine ZnO NP-exposed co-culture was significantly lower than that in hydrophobic ZnO NP-exposed co-culture (p < 0.05). In conclusion, hydrophobic surface coating might influence the colloidal aspects of ZnO NPs and alter ER stress-apoptosis-autophagy gene expression pattern by pristine ZnO NPs in A549-macrophage co-culture.

Authors+Show Affiliations

International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, PR China; Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education, Lab of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, PR China; Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education, Lab of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, PR China.Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education, Lab of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education, Lab of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, PR China. Electronic address: liao@gzucm.edu.cn.International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, PR China; Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education, Lab of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan 411105, PR China; Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, PR China. Electronic address: caoyi39@xtu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30340083

Citation

Liu, Ting, et al. "Influence of Pristine and Hydrophobic ZnO Nanoparticles On Cytotoxicity and Endoplasmic Reticulum (ER) Stress-autophagy-apoptosis Gene Expression in A549-macrophage Co-culture." Ecotoxicology and Environmental Safety, vol. 167, 2019, pp. 188-195.
Liu T, Liang H, Liu L, et al. Influence of pristine and hydrophobic ZnO nanoparticles on cytotoxicity and endoplasmic reticulum (ER) stress-autophagy-apoptosis gene expression in A549-macrophage co-culture. Ecotoxicol Environ Saf. 2019;167:188-195.
Liu, T., Liang, H., Liu, L., Gong, Y., Ding, Y., Liao, G., & Cao, Y. (2019). Influence of pristine and hydrophobic ZnO nanoparticles on cytotoxicity and endoplasmic reticulum (ER) stress-autophagy-apoptosis gene expression in A549-macrophage co-culture. Ecotoxicology and Environmental Safety, 167, 188-195. https://doi.org/10.1016/j.ecoenv.2018.10.018
Liu T, et al. Influence of Pristine and Hydrophobic ZnO Nanoparticles On Cytotoxicity and Endoplasmic Reticulum (ER) Stress-autophagy-apoptosis Gene Expression in A549-macrophage Co-culture. Ecotoxicol Environ Saf. 2019 Jan 15;167:188-195. PubMed PMID: 30340083.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of pristine and hydrophobic ZnO nanoparticles on cytotoxicity and endoplasmic reticulum (ER) stress-autophagy-apoptosis gene expression in A549-macrophage co-culture. AU - Liu,Ting, AU - Liang,Hongying, AU - Liu,Liangliang, AU - Gong,Yu, AU - Ding,Yanhuai, AU - Liao,Guochao, AU - Cao,Yi, Y1 - 2018/10/16/ PY - 2018/06/26/received PY - 2018/10/02/revised PY - 2018/10/05/accepted PY - 2018/10/20/pubmed PY - 2019/1/3/medline PY - 2018/10/20/entrez KW - A549-macrophage co-culture KW - Cytotoxicity KW - ER stress-autophagy-apoptosis gene expression KW - Soluble monocyte chemotactic protein-1 (sMCP-1) KW - ZnO nanoparticles (NPs) SP - 188 EP - 195 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 167 N2 - Exposure to ZnO nanoparticles (NPs) might modulate endoplasmic reticulum (ER) stress-autophagy gene expression, but the possible influence of hydrophobic surface coating on these responses was less studied. This study used A549-macrophage co-culture as the in vitro model for lung barrier and investigated the toxicity of pristine and hydrophobic ZnO NPs. Pristine and hydrophobic NPs exhibited different Zeta potential and solubility in water, which suggested that hydrophobic surface coating might alter the colloidal aspects of ZnO NPs. However, pristine and hydrophobic ZnO NPs induced cytotoxicity and reduced the release of soluble monocyte chemotactic protein-1 (sMCP-1) in A549-macrophage co-culture to a similar extent. Exposure to pristine ZnO NPs significantly promoted the expression of ER stress-apoptosis genes, namely DDIT3, XBP-1s, CASP9, CASP12 and BAX (p < 0.05), but hydrophobic ZnO NPs only significantly promoted the expression of BAX (p < 0.05). Exposure to pristine ZnO NPs also significantly reduced the expression of autophagic gene BECN1 (p < 0.05) but not ATG7 (p > 0.05), whereas hydrophobic ZnO NPs significantly reduced the expression of ATG7 and BECN1 (p < 0.01). Moreover, the expression of XBP-1s, HSPA5, CASP9, CASP12, BAX and ATG7 in pristine ZnO NP-exposed co-culture was significantly lower than that in hydrophobic ZnO NP-exposed co-culture (p < 0.05). In conclusion, hydrophobic surface coating might influence the colloidal aspects of ZnO NPs and alter ER stress-apoptosis-autophagy gene expression pattern by pristine ZnO NPs in A549-macrophage co-culture. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/30340083/Influence_of_pristine_and_hydrophobic_ZnO_nanoparticles_on_cytotoxicity_and_endoplasmic_reticulum__ER__stress_autophagy_apoptosis_gene_expression_in_A549_macrophage_co_culture_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(18)31019-4 DB - PRIME DP - Unbound Medicine ER -