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Osteogenic protein-1 inhibits nucleus pulposus cell apoptosis through regulating the NF-κB/ROS pathway in an inflammation environment.
Biosci Rep. 2018 12 21; 38(6)BR

Abstract

BACKGROUND

Intervertebral disc degeneration is a pathological process that involves an inflammation response. As a classical cellular feature, several studies have demonstrated that inflammation can promote nucleus pulposus (NP) cell apoptosis. Therefore, attenuation of NP cell apoptosis may be a potential way to retard disc degeneration.

OBJECTIVE

The present study was aimed to investigate the protective effects of osteogenic protein-1 (OP-1) against NP cell apoptosis in an inflammation environment, and the potential signaling transduction pathway.

METHODS

Rat NP cells were cultured in medium with or without inflammatory cytokine tumor necrosis factor (TNF)-α for 6 days. The exogenous TNF-α was added into the medium to investigate its protective effects. NP cell apoptosis was evaluated by cell apoptosis ratio, caspase-3 activity, gene/protein expression of apoptosis-related molecules (Bcl-2, Bax, and caspase-3). Additionally, the intracellular reactive oxygen species (ROS) content and activity of the NF-κB pathway were also analyzed.

RESULTS

Compared with the control NP cells, TNF-α significantly increased cell apoptosis ratio, caspase-3 activity, gene/protein expression of Bcl-2, Bax and caspase-3, ROS content, and activity of the NF-κB pathway. However, OP-1 partly attenuated these effects in NP cells treated with TNF-α.

CONCLUSION

OP-1 is effective in attenuating TNF-α-caused NP cell apoptosis, and the ROS/NF-κB pathway may be the potential signaling transduction pathway. The present study indicates that OP-1 may be helpful to inhibit inflammation-mediated disc degeneration.

Authors+Show Affiliations

Department of Orthopedics, Chongqing Medical University, Chongqing 400016, China. Department of Orthopedics, Inner Mongolia Baogang Hospital, Baotou, Neimenggu 014010, China.Department of Orthopedics, Inner Mongolia Baogang Hospital, Baotou, Neimenggu 014010, China.Department of Orthopedics, Inner Mongolia Baogang Hospital, Baotou, Neimenggu 014010, China.Department of Orthopedics, Inner Mongolia Baogang Hospital, Baotou, Neimenggu 014010, China.Department of Orthopedics, Chongqing Medical University, Chongqing 400016, China jdm1026@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30341245

Citation

Yu, Wei, et al. "Osteogenic Protein-1 Inhibits Nucleus Pulposus Cell Apoptosis Through Regulating the NF-κB/ROS Pathway in an Inflammation Environment." Bioscience Reports, vol. 38, no. 6, 2018.
Yu W, Fu J, Liu Y, et al. Osteogenic protein-1 inhibits nucleus pulposus cell apoptosis through regulating the NF-κB/ROS pathway in an inflammation environment. Biosci Rep. 2018;38(6).
Yu, W., Fu, J., Liu, Y., Wu, Y., & Jiang, D. (2018). Osteogenic protein-1 inhibits nucleus pulposus cell apoptosis through regulating the NF-κB/ROS pathway in an inflammation environment. Bioscience Reports, 38(6). https://doi.org/10.1042/BSR20181530
Yu W, et al. Osteogenic Protein-1 Inhibits Nucleus Pulposus Cell Apoptosis Through Regulating the NF-κB/ROS Pathway in an Inflammation Environment. Biosci Rep. 2018 12 21;38(6) PubMed PMID: 30341245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Osteogenic protein-1 inhibits nucleus pulposus cell apoptosis through regulating the NF-κB/ROS pathway in an inflammation environment. AU - Yu,Wei, AU - Fu,Jiabin, AU - Liu,Yan, AU - Wu,Yuchi, AU - Jiang,Dianming, Y1 - 2018/11/20/ PY - 2018/09/03/received PY - 2018/10/04/revised PY - 2018/10/11/accepted PY - 2018/10/21/pubmed PY - 2019/7/20/medline PY - 2018/10/21/entrez KW - TNF-α KW - apoptosis KW - disc degeneration KW - nucleus pulposus KW - osteogenic protein-1 JF - Bioscience reports JO - Biosci Rep VL - 38 IS - 6 N2 - BACKGROUND: Intervertebral disc degeneration is a pathological process that involves an inflammation response. As a classical cellular feature, several studies have demonstrated that inflammation can promote nucleus pulposus (NP) cell apoptosis. Therefore, attenuation of NP cell apoptosis may be a potential way to retard disc degeneration. OBJECTIVE: The present study was aimed to investigate the protective effects of osteogenic protein-1 (OP-1) against NP cell apoptosis in an inflammation environment, and the potential signaling transduction pathway. METHODS: Rat NP cells were cultured in medium with or without inflammatory cytokine tumor necrosis factor (TNF)-α for 6 days. The exogenous TNF-α was added into the medium to investigate its protective effects. NP cell apoptosis was evaluated by cell apoptosis ratio, caspase-3 activity, gene/protein expression of apoptosis-related molecules (Bcl-2, Bax, and caspase-3). Additionally, the intracellular reactive oxygen species (ROS) content and activity of the NF-κB pathway were also analyzed. RESULTS: Compared with the control NP cells, TNF-α significantly increased cell apoptosis ratio, caspase-3 activity, gene/protein expression of Bcl-2, Bax and caspase-3, ROS content, and activity of the NF-κB pathway. However, OP-1 partly attenuated these effects in NP cells treated with TNF-α. CONCLUSION: OP-1 is effective in attenuating TNF-α-caused NP cell apoptosis, and the ROS/NF-κB pathway may be the potential signaling transduction pathway. The present study indicates that OP-1 may be helpful to inhibit inflammation-mediated disc degeneration. SN - 1573-4935 UR - https://www.unboundmedicine.com/medline/citation/30341245/Osteogenic_protein_1_inhibits_nucleus_pulposus_cell_apoptosis_through_regulating_the_NF_κB/ROS_pathway_in_an_inflammation_environment_ L2 - https://portlandpress.com/bioscirep/article-lookup/doi/10.1042/BSR20181530 DB - PRIME DP - Unbound Medicine ER -