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Antidiabetic activities of chloroform fraction of Anthocleista vogelii Planch root bark in rats with diet- and alloxan-induced obesity-diabetes.
J Ethnopharmacol. 2019 Jan 30; 229:293-302.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Anthocleista vogelii Planch is a medicinal plant traditionally used in West Africa for the management and treatment of diabetes mellitus.

AIM OF THE STUDY

To determine the antidiabetic activities of chloroform fraction (CF) of Anthocleista vogelii Planch root bark in rats with diet- and alloxan-induced obesity-diabetes.

MATERIALS AND METHODS

Inhibitory activities of CF against α-amylase and α-glucosidase activities were determined in vitro. Three weeks old rats were fed with high-fat diet for 9 weeks to induce obesity prior to further induction of diabetes using alloxan (150 mg/kg body weight, i.p.). Blood glucose levels and body weight were measured every 7 days throughout the experiment. Glucose tolerance was assessed in normal and CF-treated rats on day 21. Terminal blood samples were collected from sacrificed animals for the measurement of serum insulin levels. Pancreases were excised from treated and untreated animals for histopathological examination.

RESULTS

LCMS/MS chromatographic profile of CF via positive and negative modes revealed 13 and 23 compounds respectively. Further analysis revealed quebrachitol (QCT), loganin, sweroside, oleoside 11-methyl ester and ferulic acid, which have been previously reported for their antidiabetic activities, as constituents of CF. CF inhibited activities of α-amylase (IC50 = 51.60 ± 0.92 µg/ml) and α-glucosidase (IC50 = 5.86 ± 0.97 µg/ml) in a dose-dependent manner. Treatment of animals with obesity-diabetes with 100 and 200 mg/kg CF significantly improved glucose tolerance (P < 0.001) and enhanced serum insulin levels (P < 0.05) compared to diabetic control rats.

CONCLUSIONS

Antidiabetic activities of CF might be mediated via inhibition of α-amylase and α-glucosidase activities, elevation of serum insulin concentration, and enhancement of insulin and leptin sensitivity in obesity-diabetes rats. This study further substantiates the traditional use of A. vogelii in the management and treatment of diabetes in Africa and encourages further studies to investigate its mechanism of action.

Authors+Show Affiliations

Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, KP, Pakistan. Electronic address: gabrielanyanwu@binghamuni.edu.ng.Centre for Advanced Drug Research, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, KP, Pakistan.School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia.Centre for Advanced Drug Research, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, KP, Pakistan.Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, KP, Pakistan.Department of Biochemistry, University of Benin, Benin City, Edo State, Nigeria.Biotechnology, School of Sciences, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton, UK.Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, KP, Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30342966

Citation

Anyanwu, Gabriel O., et al. "Antidiabetic Activities of Chloroform Fraction of Anthocleista Vogelii Planch Root Bark in Rats With Diet- and Alloxan-induced Obesity-diabetes." Journal of Ethnopharmacology, vol. 229, 2019, pp. 293-302.
Anyanwu GO, Iqbal J, Khan SU, et al. Antidiabetic activities of chloroform fraction of Anthocleista vogelii Planch root bark in rats with diet- and alloxan-induced obesity-diabetes. J Ethnopharmacol. 2019;229:293-302.
Anyanwu, G. O., Iqbal, J., Khan, S. U., Zaib, S., Rauf, K., Onyeneke, C. E., Ojo, O. O., & Nisar-Ur-Rahman, . (2019). Antidiabetic activities of chloroform fraction of Anthocleista vogelii Planch root bark in rats with diet- and alloxan-induced obesity-diabetes. Journal of Ethnopharmacology, 229, 293-302. https://doi.org/10.1016/j.jep.2018.10.021
Anyanwu GO, et al. Antidiabetic Activities of Chloroform Fraction of Anthocleista Vogelii Planch Root Bark in Rats With Diet- and Alloxan-induced Obesity-diabetes. J Ethnopharmacol. 2019 Jan 30;229:293-302. PubMed PMID: 30342966.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antidiabetic activities of chloroform fraction of Anthocleista vogelii Planch root bark in rats with diet- and alloxan-induced obesity-diabetes. AU - Anyanwu,Gabriel O, AU - Iqbal,Jamshed, AU - Khan,Shafi U, AU - Zaib,Sumera, AU - Rauf,Khalid, AU - Onyeneke,Chukwu E, AU - Ojo,Opeolu O, AU - Nisar-Ur-Rahman,, Y1 - 2018/10/18/ PY - 2018/05/17/received PY - 2018/10/01/revised PY - 2018/10/14/accepted PY - 2018/10/22/pubmed PY - 2018/12/26/medline PY - 2018/10/22/entrez KW - (+)-Maackiain 3-O-glucoside (PubChem CID: 44257441) KW - 3-Hydroxy-3-methyl-glutaric acid (PubChem CID: 1662) KW - 3-bromo-2E-acrylic acid (not found) KW - 5,7-Dihydroxy-3',4'-dimethoxy-6,8-dimethylflavone (not found) KW - 5,7-Dihydroxy-3-methoxy-4'-prenyloxyflavone (not found) KW - 5-Acetylamino-6-formylamino-3-methyluracil (PubChem CID: 108214) KW - Alloxan KW - Anthocleista vogelii KW - Antidiabetic KW - Apigenin 7-methyl ether 5-glucoside (not found) KW - C16 Sphinganine (PubChem CID: 70679052) KW - D-Glucoside (PubChem CID: 64947) KW - Di-alpha-furfuryl ether (PubChem CID: 263034) KW - Emmotin A (PubChem CID: 42608142) KW - Ferulic acid (PubChem CID: 445858) KW - Galactopinitol B (PubChem CID: 101926786) KW - LCMS/MS KW - Loganin (PubChem CID: 87691) KW - Methylorsellinic Acid, Ethyl Ester (PubChem CID: 3084545) KW - N-hexanoyl-L-Homoserine lactone (PubChem CID: 10058590) KW - Oleamide (PubChem CID: 5283387) KW - Oleoside 11-methyl ester (PubChem CID: 10692563) KW - Phellodensin D (PubChem CID: 5316782) KW - Phlorisobutyrophenone (PubChem CID: 5326317) KW - Phytosphingosine (PubChem CID: 122121) KW - Quebrachitol (PubChem CID: 151108) KW - Samidin (PubChem CID: 442150) KW - Sweroside (PubChem CID: 161036) KW - Theobromine (PubChem CID: 5429) KW - Undecanedioic acid (PubChem CID: 15816) KW - alpha-Bromocrotonic acid (PubChem CID: 5312952) KW - gamma-Thujaplicin (PubChem CID: 12649) KW - α-amylase KW - α-glucosidase SP - 293 EP - 302 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 229 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Anthocleista vogelii Planch is a medicinal plant traditionally used in West Africa for the management and treatment of diabetes mellitus. AIM OF THE STUDY: To determine the antidiabetic activities of chloroform fraction (CF) of Anthocleista vogelii Planch root bark in rats with diet- and alloxan-induced obesity-diabetes. MATERIALS AND METHODS: Inhibitory activities of CF against α-amylase and α-glucosidase activities were determined in vitro. Three weeks old rats were fed with high-fat diet for 9 weeks to induce obesity prior to further induction of diabetes using alloxan (150 mg/kg body weight, i.p.). Blood glucose levels and body weight were measured every 7 days throughout the experiment. Glucose tolerance was assessed in normal and CF-treated rats on day 21. Terminal blood samples were collected from sacrificed animals for the measurement of serum insulin levels. Pancreases were excised from treated and untreated animals for histopathological examination. RESULTS: LCMS/MS chromatographic profile of CF via positive and negative modes revealed 13 and 23 compounds respectively. Further analysis revealed quebrachitol (QCT), loganin, sweroside, oleoside 11-methyl ester and ferulic acid, which have been previously reported for their antidiabetic activities, as constituents of CF. CF inhibited activities of α-amylase (IC50 = 51.60 ± 0.92 µg/ml) and α-glucosidase (IC50 = 5.86 ± 0.97 µg/ml) in a dose-dependent manner. Treatment of animals with obesity-diabetes with 100 and 200 mg/kg CF significantly improved glucose tolerance (P < 0.001) and enhanced serum insulin levels (P < 0.05) compared to diabetic control rats. CONCLUSIONS: Antidiabetic activities of CF might be mediated via inhibition of α-amylase and α-glucosidase activities, elevation of serum insulin concentration, and enhancement of insulin and leptin sensitivity in obesity-diabetes rats. This study further substantiates the traditional use of A. vogelii in the management and treatment of diabetes in Africa and encourages further studies to investigate its mechanism of action. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/30342966/Antidiabetic_activities_of_chloroform_fraction_of_Anthocleista_vogelii_Planch_root_bark_in_rats_with_diet__and_alloxan_induced_obesity_diabetes_ DB - PRIME DP - Unbound Medicine ER -