Tags

Type your tag names separated by a space and hit enter

Transfer RNA-derived fragments as potential exosome tRNA-derived fragment biomarkers for osteoporosis.
Int J Rheum Dis 2018; 21(9):1659-1669IJ

Abstract

AIM

Osteoporosis is one of the common orthopedic diseases featured in low bone mineral density. Exosomes have been proven to be potential markers for many diseases and health problems. The roles of messenger RNAs and microRNAs in osteoporosis have been comprehensively studied; however, little research has focused on the function of plasma exosomal transfer RNA-derived fragments (tRFs) in osteoporosis.

METHODS

We collected plasma samples from 40 healthy controls and 40 osteoporosis patients, and all exosomes were isolated with combined centrifugation and were characterized by electron microscopy. Small RNA sequence (Yingbio) was performed to detect the plasma exosomal tRFs and tRF markers were validated by real-time quantitative polymerase chain reaction (qPCR). Three exosome diagnostic tRFs were confirmed by receiver operating characteristic analyses.

RESULTS

In this study, 11 upregulated tRFs and 18 downregulated tRFs were identified in osteoporosis compared with normal controls. Higher expression levels of plasma exosomal tRF-25-R9ODMJ6B26 (tRF-25), tRF-38-QB1MK8YUBS68BFD2 (tRF-38), tRF-18-BS68BFD2 (tRF-18) in osteoporosis were confirmed by qPCR. Plasma exosomal tRF-25, tRF-38 and tRF-18 showed better accuracy for osteoporosis diagnosis.

CONCLUSION

Our results suggest that plasma exosomal tRF-25, tRF-38 and tRF-18 might be diagnostic biomarkers for osteoporosis detection.

Authors+Show Affiliations

Department of Orthopedics, Shanghai Yangpu Hospital Affiliated to Tongji University, Shanghai, China.Department of Orthopedics, Shanghai Yangpu Hospital Affiliated to Tongji University, Shanghai, China.Department of Orthopedics, Shanghai Zhoupu Hospital, Shanghai, China.Department of Orthopedics, Shanghai Yangpu Hospital Affiliated to Tongji University, Shanghai, China.Department of Orthopedics, Shanghai Yangpu Hospital Affiliated to Tongji University, Shanghai, China.Department of Orthopedics, Shanghai Yangpu Hospital Affiliated to Tongji University, Shanghai, China.Department of Orthopedics, Shanghai Yangpu Hospital Affiliated to Tongji University, Shanghai, China. Department of Orthopedics, Shanghai Zhoupu Hospital, Shanghai, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30345646

Citation

Zhang, Yan, et al. "Transfer RNA-derived Fragments as Potential Exosome tRNA-derived Fragment Biomarkers for Osteoporosis." International Journal of Rheumatic Diseases, vol. 21, no. 9, 2018, pp. 1659-1669.
Zhang Y, Cai F, Liu J, et al. Transfer RNA-derived fragments as potential exosome tRNA-derived fragment biomarkers for osteoporosis. Int J Rheum Dis. 2018;21(9):1659-1669.
Zhang, Y., Cai, F., Liu, J., Chang, H., Liu, L., Yang, A., & Liu, X. (2018). Transfer RNA-derived fragments as potential exosome tRNA-derived fragment biomarkers for osteoporosis. International Journal of Rheumatic Diseases, 21(9), pp. 1659-1669. doi:10.1111/1756-185X.13346.
Zhang Y, et al. Transfer RNA-derived Fragments as Potential Exosome tRNA-derived Fragment Biomarkers for Osteoporosis. Int J Rheum Dis. 2018;21(9):1659-1669. PubMed PMID: 30345646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transfer RNA-derived fragments as potential exosome tRNA-derived fragment biomarkers for osteoporosis. AU - Zhang,Yan, AU - Cai,Feng, AU - Liu,Ju, AU - Chang,Hongze, AU - Liu,Liang, AU - Yang,Anli, AU - Liu,Xiaodong, PY - 2018/10/23/entrez PY - 2018/10/23/pubmed PY - 2019/3/15/medline KW - diagnostic biomarker KW - exosome KW - osteoporosis KW - plasma KW - receiver operating characteristic KW - transfer RNA-derived fragment SP - 1659 EP - 1669 JF - International journal of rheumatic diseases JO - Int J Rheum Dis VL - 21 IS - 9 N2 - AIM: Osteoporosis is one of the common orthopedic diseases featured in low bone mineral density. Exosomes have been proven to be potential markers for many diseases and health problems. The roles of messenger RNAs and microRNAs in osteoporosis have been comprehensively studied; however, little research has focused on the function of plasma exosomal transfer RNA-derived fragments (tRFs) in osteoporosis. METHODS: We collected plasma samples from 40 healthy controls and 40 osteoporosis patients, and all exosomes were isolated with combined centrifugation and were characterized by electron microscopy. Small RNA sequence (Yingbio) was performed to detect the plasma exosomal tRFs and tRF markers were validated by real-time quantitative polymerase chain reaction (qPCR). Three exosome diagnostic tRFs were confirmed by receiver operating characteristic analyses. RESULTS: In this study, 11 upregulated tRFs and 18 downregulated tRFs were identified in osteoporosis compared with normal controls. Higher expression levels of plasma exosomal tRF-25-R9ODMJ6B26 (tRF-25), tRF-38-QB1MK8YUBS68BFD2 (tRF-38), tRF-18-BS68BFD2 (tRF-18) in osteoporosis were confirmed by qPCR. Plasma exosomal tRF-25, tRF-38 and tRF-18 showed better accuracy for osteoporosis diagnosis. CONCLUSION: Our results suggest that plasma exosomal tRF-25, tRF-38 and tRF-18 might be diagnostic biomarkers for osteoporosis detection. SN - 1756-185X UR - https://www.unboundmedicine.com/medline/citation/30345646/Transfer_RNA_derived_fragments_as_potential_exosome_tRNA_derived_fragment_biomarkers_for_osteoporosis_ L2 - https://doi.org/10.1111/1756-185X.13346 DB - PRIME DP - Unbound Medicine ER -