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Proteomics analysis to compare the venom composition between Naja naja and Naja kaouthia from the same geographical location of eastern India: Correlation with pathophysiology of envenomation and immunological cross-reactivity towards commercial polyantivenom.
Expert Rev Proteomics. 2018 11; 15(11):949-961.ER

Abstract

BACKGROUND

Cobra bite is frequently reported across the Indian subcontinent and is associated with a high rate of death and morbidity. In eastern India (EI) Naja naja and Naja kaouthia are reported to be the two most abundant species of cobra.

RESEARCH DESIGN AND METHODS

The venom proteome composition of N. naja (NnV) and N. kaouthia (NkV) from Burdwan districts of EI were compared by separation of venom proteins by 1D-SDS-PAGE followed by LC-MS/MS analysis of protein bands. The potency of commercial polyantivenom (PAV) was assessed by neutralization, ELISA, immuno-blot and venom-PAV immunoaffinity chromatography studies.

RESULTS

Proteomic analysis identified 52 and 55 proteins for NnV and NkV, respectively, when searched against the Elapidae database. A small quantitative difference in venom composition between these two species of cobra was observed. PAVs exhibited poor cross-reactivity against low molecular mass toxins (<20 kDa) of both cobra venoms, which was substantiated by a meager neutralization of their phospholipase A2 activity. Phospholipase A2 and 3FTx, the two major classes of nonenzymatic and enzymatic proteins, respectively, were partially recognized by PAVs.

CONCLUSIONS

Efforts must be made to improve immunization protocols and supplement existing antivenoms with antibodies raised against the major toxins of these venoms.

Authors+Show Affiliations

a Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology , Tezpur University , Tezpur , India.a Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology , Tezpur University , Tezpur , India.a Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology , Tezpur University , Tezpur , India.a Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology , Tezpur University , Tezpur , India.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media

Language

eng

PubMed ID

30345852

Citation

Chanda, Abhishek, et al. "Proteomics Analysis to Compare the Venom Composition Between Naja Naja and Naja Kaouthia From the Same Geographical Location of Eastern India: Correlation With Pathophysiology of Envenomation and Immunological Cross-reactivity Towards Commercial Polyantivenom." Expert Review of Proteomics, vol. 15, no. 11, 2018, pp. 949-961.
Chanda A, Patra A, Kalita B, et al. Proteomics analysis to compare the venom composition between Naja naja and Naja kaouthia from the same geographical location of eastern India: Correlation with pathophysiology of envenomation and immunological cross-reactivity towards commercial polyantivenom. Expert Rev Proteomics. 2018;15(11):949-961.
Chanda, A., Patra, A., Kalita, B., & Mukherjee, A. K. (2018). Proteomics analysis to compare the venom composition between Naja naja and Naja kaouthia from the same geographical location of eastern India: Correlation with pathophysiology of envenomation and immunological cross-reactivity towards commercial polyantivenom. Expert Review of Proteomics, 15(11), 949-961. https://doi.org/10.1080/14789450.2018.1538799
Chanda A, et al. Proteomics Analysis to Compare the Venom Composition Between Naja Naja and Naja Kaouthia From the Same Geographical Location of Eastern India: Correlation With Pathophysiology of Envenomation and Immunological Cross-reactivity Towards Commercial Polyantivenom. Expert Rev Proteomics. 2018;15(11):949-961. PubMed PMID: 30345852.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomics analysis to compare the venom composition between Naja naja and Naja kaouthia from the same geographical location of eastern India: Correlation with pathophysiology of envenomation and immunological cross-reactivity towards commercial polyantivenom. AU - Chanda,Abhishek, AU - Patra,Aparup, AU - Kalita,Bhargab, AU - Mukherjee,Ashis K, Y1 - 2018/10/31/ PY - 2018/10/23/pubmed PY - 2019/6/25/medline PY - 2018/10/23/entrez KW - Antivenomics KW - cobra venom KW - snake venom KW - snakebite KW - venom-antivenom cross-reactivity SP - 949 EP - 961 JF - Expert review of proteomics JO - Expert Rev Proteomics VL - 15 IS - 11 N2 - BACKGROUND: Cobra bite is frequently reported across the Indian subcontinent and is associated with a high rate of death and morbidity. In eastern India (EI) Naja naja and Naja kaouthia are reported to be the two most abundant species of cobra. RESEARCH DESIGN AND METHODS: The venom proteome composition of N. naja (NnV) and N. kaouthia (NkV) from Burdwan districts of EI were compared by separation of venom proteins by 1D-SDS-PAGE followed by LC-MS/MS analysis of protein bands. The potency of commercial polyantivenom (PAV) was assessed by neutralization, ELISA, immuno-blot and venom-PAV immunoaffinity chromatography studies. RESULTS: Proteomic analysis identified 52 and 55 proteins for NnV and NkV, respectively, when searched against the Elapidae database. A small quantitative difference in venom composition between these two species of cobra was observed. PAVs exhibited poor cross-reactivity against low molecular mass toxins (<20 kDa) of both cobra venoms, which was substantiated by a meager neutralization of their phospholipase A2 activity. Phospholipase A2 and 3FTx, the two major classes of nonenzymatic and enzymatic proteins, respectively, were partially recognized by PAVs. CONCLUSIONS: Efforts must be made to improve immunization protocols and supplement existing antivenoms with antibodies raised against the major toxins of these venoms. SN - 1744-8387 UR - https://www.unboundmedicine.com/medline/citation/30345852/Proteomics_analysis_to_compare_the_venom_composition_between_Naja_naja_and_Naja_kaouthia_from_the_same_geographical_location_of_eastern_India:_Correlation_with_pathophysiology_of_envenomation_and_immunological_cross_reactivity_towards_commercial_polyantivenom_ L2 - http://www.tandfonline.com/doi/full/10.1080/14789450.2018.1538799 DB - PRIME DP - Unbound Medicine ER -