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Quantification of THC in Cannabis plants by fast-HPLC-DAD: A promising method for routine analyses.
Talanta. 2019 Jan 15; 192:135-141.T

Abstract

Nowadays, Gas Chromatography Mass Spectrometry (GC-MS) is mainly used in forensic sciences but suffers from limitations when the analysed compounds are thermally instable as it is the case for THC-A (Tetrahydrocannabinolic Acid) which is converted into Δ9-THC (Δ9-Tetrahydrocannabinol) that subsequently partially degrades. We propose herein a Fast High Pressure Liquid Chromatography (Fast-HPLC-DAD) method which allows the efficient separation of CBN (Cannabinol), CBD (Cannabidiol), THC-A and Δ9-THC, the major cannabinoids compounds found in cannabis plants in less than 5 min. Our method allows also the proper quantification of Δ9-THC in plant extracts using an external calibration method with a very good accuracy as pointed out by a recovery of 100.53 ± 3.12%. It is also an interesting low cost alternative to Ultra High Pressure Liquid Chromatography (UPLC) for routine analyses in forensic sciences.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Burnier C
School of Criminal Justice, University of Lausanne, Switzerland.
Esseiva P
School of Criminal Justice, University of Lausanne, Switzerland.
Roussel C
Section of Chemistry and Chemical Engineering, Ecole Polytechnique Fédérale de Lausanne, Switzerland. Electronic address: christophe.roussel@epfl.ch.

MeSH

CalibrationCannabisChromatography, High Pressure LiquidDronabinolGas Chromatography-Mass SpectrometryPlant Extracts

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30348368

Citation

Burnier, Céline, et al. "Quantification of THC in Cannabis Plants By fast-HPLC-DAD: a Promising Method for Routine Analyses." Talanta, vol. 192, 2019, pp. 135-141.
Burnier C, Esseiva P, Roussel C. Quantification of THC in Cannabis plants by fast-HPLC-DAD: A promising method for routine analyses. Talanta. 2019;192:135-141.
Burnier, C., Esseiva, P., & Roussel, C. (2019). Quantification of THC in Cannabis plants by fast-HPLC-DAD: A promising method for routine analyses. Talanta, 192, 135-141. https://doi.org/10.1016/j.talanta.2018.09.012
Burnier C, Esseiva P, Roussel C. Quantification of THC in Cannabis Plants By fast-HPLC-DAD: a Promising Method for Routine Analyses. Talanta. 2019 Jan 15;192:135-141. PubMed PMID: 30348368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of THC in Cannabis plants by fast-HPLC-DAD: A promising method for routine analyses. AU - Burnier,Céline, AU - Esseiva,Pierre, AU - Roussel,Christophe, Y1 - 2018/09/06/ PY - 2018/03/28/received PY - 2018/08/31/revised PY - 2018/09/04/accepted PY - 2018/10/24/entrez PY - 2018/10/24/pubmed PY - 2018/11/21/medline KW - Cannabis KW - Fast-HPLC-DAD KW - THC-A KW - Δ(9)-THC SP - 135 EP - 141 JF - Talanta JO - Talanta VL - 192 N2 - Nowadays, Gas Chromatography Mass Spectrometry (GC-MS) is mainly used in forensic sciences but suffers from limitations when the analysed compounds are thermally instable as it is the case for THC-A (Tetrahydrocannabinolic Acid) which is converted into Δ9-THC (Δ9-Tetrahydrocannabinol) that subsequently partially degrades. We propose herein a Fast High Pressure Liquid Chromatography (Fast-HPLC-DAD) method which allows the efficient separation of CBN (Cannabinol), CBD (Cannabidiol), THC-A and Δ9-THC, the major cannabinoids compounds found in cannabis plants in less than 5 min. Our method allows also the proper quantification of Δ9-THC in plant extracts using an external calibration method with a very good accuracy as pointed out by a recovery of 100.53 ± 3.12%. It is also an interesting low cost alternative to Ultra High Pressure Liquid Chromatography (UPLC) for routine analyses in forensic sciences. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/30348368/Quantification_of_THC_in_Cannabis_plants_by_fast_HPLC_DAD:_A_promising_method_for_routine_analyses_ DB - PRIME DP - Unbound Medicine ER -