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Sodium-potassium pump, ion fluxes, and cellular dehydration in sickle cell anemia.
J Clin Invest 1987; 79(6):1621-8JCI

Abstract

We studied the role of the sodium-potassium pump in erythrocytes of 12 patients with sickle cell anemia (SS). Ouabain-binding sites per cell and pump-mediated Rb/K uptake were significantly higher in SS patients than in white or black controls. Ouabain-resistant Rb/K influx was also greater than in normal controls or patients with sickle cell trait. Deoxygenation of SS erythrocytes increased ouabain-sensitive Rb/K influx without altering ouabain binding, presumably as the consequence of an increase in the passive influx of sodium. Deoxygenation increased mean corpuscular hemoglobin concentration (MCHC) by 5.5%, and studies of the density distribution of SS cells indicated an increase in highly dense fractions known to contain sickled erythrocytes. Ouabain prevented the rise in MCHC and reduced the percentage of dense cells. These findings indicate a magnified role for the sodium-potassium pump in the pathophysiology of SS erythrocytes and suggest that its inhibition might prove useful in therapy.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3034977

Citation

Izumo, H, et al. "Sodium-potassium Pump, Ion Fluxes, and Cellular Dehydration in Sickle Cell Anemia." The Journal of Clinical Investigation, vol. 79, no. 6, 1987, pp. 1621-8.
Izumo H, Lear S, Williams M, et al. Sodium-potassium pump, ion fluxes, and cellular dehydration in sickle cell anemia. J Clin Invest. 1987;79(6):1621-8.
Izumo, H., Lear, S., Williams, M., Rosa, R., & Epstein, F. H. (1987). Sodium-potassium pump, ion fluxes, and cellular dehydration in sickle cell anemia. The Journal of Clinical Investigation, 79(6), pp. 1621-8.
Izumo H, et al. Sodium-potassium Pump, Ion Fluxes, and Cellular Dehydration in Sickle Cell Anemia. J Clin Invest. 1987;79(6):1621-8. PubMed PMID: 3034977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sodium-potassium pump, ion fluxes, and cellular dehydration in sickle cell anemia. AU - Izumo,H, AU - Lear,S, AU - Williams,M, AU - Rosa,R, AU - Epstein,F H, PY - 1987/6/1/pubmed PY - 1987/6/1/medline PY - 1987/6/1/entrez SP - 1621 EP - 8 JF - The Journal of clinical investigation JO - J. Clin. Invest. VL - 79 IS - 6 N2 - We studied the role of the sodium-potassium pump in erythrocytes of 12 patients with sickle cell anemia (SS). Ouabain-binding sites per cell and pump-mediated Rb/K uptake were significantly higher in SS patients than in white or black controls. Ouabain-resistant Rb/K influx was also greater than in normal controls or patients with sickle cell trait. Deoxygenation of SS erythrocytes increased ouabain-sensitive Rb/K influx without altering ouabain binding, presumably as the consequence of an increase in the passive influx of sodium. Deoxygenation increased mean corpuscular hemoglobin concentration (MCHC) by 5.5%, and studies of the density distribution of SS cells indicated an increase in highly dense fractions known to contain sickled erythrocytes. Ouabain prevented the rise in MCHC and reduced the percentage of dense cells. These findings indicate a magnified role for the sodium-potassium pump in the pathophysiology of SS erythrocytes and suggest that its inhibition might prove useful in therapy. SN - 0021-9738 UR - https://www.unboundmedicine.com/medline/citation/3034977/Sodium_potassium_pump_ion_fluxes_and_cellular_dehydration_in_sickle_cell_anemia_ L2 - https://doi.org/10.1172/JCI112998 DB - PRIME DP - Unbound Medicine ER -