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Tissue-specific transcription of proteases and nucleases across the accessory salivary gland, principal salivary gland and gut of Nezara viridula.
Insect Biochem Mol Biol. 2018 12; 103:36-45.IB

Abstract

The phytophagous stink bug, Nezara viridula (L.) infests multiple plant species and impacts agricultural production worldwide. We analyzed the transcriptomes of N. viridula accessory salivary gland (ASG), principal salivary gland (PSG) and gut, with a focus on putative digestive proteases and nucleases that present a primary obstacle for the stability of protein- or nucleic acid-based stink bug control approaches. We performed high throughput Illumina sequencing followed by de novo transcriptome assemblies. We identified the sequences of 141 unique proteases and 134 nucleases from the N. viridula transcriptomes. Analysis of relative transcript abundance in conjunction with previously reported proteome data (Lomate and Bonning, 2016) supports high levels of serine protease expression in the salivary glands and high cysteine protease expression in the gut. Specifically, trypsin and chymotrypsin transcripts were abundant in the PSG, and cathepsin L-like cysteine protease transcripts were abundant in the gut. Nuclease transcript levels were generally lower than those of the proteases, the exception being abundant transcripts of ribonuclease-C20 in the PSG. The abundance of chymotrypsin, trypsin, and some carboxypeptidase transcripts suggests a significant role for the PSG in production of digestive enzymes. This result is at odds with the premise that the ASG produces watery saliva, which is high in enzymatic activity, while the PSG produces only sheath saliva. We have generated a comprehensive transcriptome sequence dataset from the digestive organs of N. viridula, identified major protease and nuclease genes and confirmed expression of the most abundant enzymes thereby providing greater insight into the digestive physiology of N. viridula.

Authors+Show Affiliations

Department of Entomology, Iowa State University, Ames, 50011, IA, USA.Department of Entomology, Iowa State University, Ames, 50011, IA, USA.Department of Entomology, Iowa State University, Ames, 50011, IA, USA. Electronic address: bbonning@ufl.edu.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30352260

Citation

Liu, Sijun, et al. "Tissue-specific Transcription of Proteases and Nucleases Across the Accessory Salivary Gland, Principal Salivary Gland and Gut of Nezara Viridula." Insect Biochemistry and Molecular Biology, vol. 103, 2018, pp. 36-45.
Liu S, Lomate PR, Bonning BC. Tissue-specific transcription of proteases and nucleases across the accessory salivary gland, principal salivary gland and gut of Nezara viridula. Insect Biochem Mol Biol. 2018;103:36-45.
Liu, S., Lomate, P. R., & Bonning, B. C. (2018). Tissue-specific transcription of proteases and nucleases across the accessory salivary gland, principal salivary gland and gut of Nezara viridula. Insect Biochemistry and Molecular Biology, 103, 36-45. https://doi.org/10.1016/j.ibmb.2018.10.003
Liu S, Lomate PR, Bonning BC. Tissue-specific Transcription of Proteases and Nucleases Across the Accessory Salivary Gland, Principal Salivary Gland and Gut of Nezara Viridula. Insect Biochem Mol Biol. 2018;103:36-45. PubMed PMID: 30352260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue-specific transcription of proteases and nucleases across the accessory salivary gland, principal salivary gland and gut of Nezara viridula. AU - Liu,Sijun, AU - Lomate,Purushottam R, AU - Bonning,Bryony C, Y1 - 2018/10/21/ PY - 2018/04/13/received PY - 2018/07/30/revised PY - 2018/10/19/accepted PY - 2018/10/24/pubmed PY - 2019/5/22/medline PY - 2018/10/24/entrez KW - Digestion KW - Nezara viridula KW - Nucleases KW - Proteases KW - Stink bugs KW - Transcriptome SP - 36 EP - 45 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 103 N2 - The phytophagous stink bug, Nezara viridula (L.) infests multiple plant species and impacts agricultural production worldwide. We analyzed the transcriptomes of N. viridula accessory salivary gland (ASG), principal salivary gland (PSG) and gut, with a focus on putative digestive proteases and nucleases that present a primary obstacle for the stability of protein- or nucleic acid-based stink bug control approaches. We performed high throughput Illumina sequencing followed by de novo transcriptome assemblies. We identified the sequences of 141 unique proteases and 134 nucleases from the N. viridula transcriptomes. Analysis of relative transcript abundance in conjunction with previously reported proteome data (Lomate and Bonning, 2016) supports high levels of serine protease expression in the salivary glands and high cysteine protease expression in the gut. Specifically, trypsin and chymotrypsin transcripts were abundant in the PSG, and cathepsin L-like cysteine protease transcripts were abundant in the gut. Nuclease transcript levels were generally lower than those of the proteases, the exception being abundant transcripts of ribonuclease-C20 in the PSG. The abundance of chymotrypsin, trypsin, and some carboxypeptidase transcripts suggests a significant role for the PSG in production of digestive enzymes. This result is at odds with the premise that the ASG produces watery saliva, which is high in enzymatic activity, while the PSG produces only sheath saliva. We have generated a comprehensive transcriptome sequence dataset from the digestive organs of N. viridula, identified major protease and nuclease genes and confirmed expression of the most abundant enzymes thereby providing greater insight into the digestive physiology of N. viridula. SN - 1879-0240 UR - https://www.unboundmedicine.com/medline/citation/30352260/Tissue_specific_transcription_of_proteases_and_nucleases_across_the_accessory_salivary_gland_principal_salivary_gland_and_gut_of_Nezara_viridula_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(18)30145-0 DB - PRIME DP - Unbound Medicine ER -