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Volumetric absorptive microsampling as an alternative sampling strategy for the determination of paracetamol in blood and cerebrospinal fluid.
Anal Bioanal Chem 2019; 411(1):181-191AB

Abstract

In the field of bioanalysis, dried matrix spot sampling is increasingly receiving interest, as this alternative sampling strategy offers many potential benefits over traditional sampling, including matrix volume-sparing properties. By using a microsampling strategy, e.g., volumetric absorptive microsampling (VAMS), the number of samples that can be collected from a patient can be increased, as a result of the limited sample volume that is required per sample. To date, no VAMS-based methods have been developed for the quantification of analytes in cerebrospinal fluid (CSF). The objective of this study was to develop and validate two LC-MS/MS methods for the quantification of paracetamol in dried blood and dried CSF, with both matrices sampled using VAMS. Both methods were fully validated based on internationally accepted guidelines. Paracetamol was chromatographically separated from its glucuronide and sulfate metabolites and no carry-over or unacceptable interferences were detected. The total precision (%RSD) was below 15% for all QC levels and accuracy (%bias) was below 7% (17% for the LLOQ of aqueous VAMS). The influence of the hematocrit on the recovery of blood VAMS samples appeared to be limited within the hematocrit range of 0.21 to 0.62. The blood VAMS samples were stable for 1 week if stored at 50 °C, and for at least 8 months when stored between - 80 °C and room temperature. The aqueous VAMS samples were stable for at least 9 months when stored between - 80 and 4 °C, and for 1 month when stored at room temperature. Application of the methods on external quality control material and analysis of patient samples demonstrated the validity and utility of the methods and provided a proof of concept for the analysis of CSF microsamples obtained via VAMS devices. Graphical abstract ᅟ.

Authors+Show Affiliations

Laboratory of Toxicology, Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium.Department of Pediatric Intensive Care, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium. Heymans Institute of Pharmacology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.Department of Pediatric Intensive Care, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium. Heymans Institute of Pharmacology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium. Department of Pharmacy, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium.Heymans Institute of Pharmacology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium. Department of Emergency Medicine, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium.Laboratory of Toxicology, Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium. christophe.stove@ugent.be.

Pub Type(s)

Journal Article
Validation Studies

Language

eng

PubMed ID

30353218

Citation

Delahaye, Lisa, et al. "Volumetric Absorptive Microsampling as an Alternative Sampling Strategy for the Determination of Paracetamol in Blood and Cerebrospinal Fluid." Analytical and Bioanalytical Chemistry, vol. 411, no. 1, 2019, pp. 181-191.
Delahaye L, Dhont E, De Cock P, et al. Volumetric absorptive microsampling as an alternative sampling strategy for the determination of paracetamol in blood and cerebrospinal fluid. Anal Bioanal Chem. 2019;411(1):181-191.
Delahaye, L., Dhont, E., De Cock, P., De Paepe, P., & Stove, C. P. (2019). Volumetric absorptive microsampling as an alternative sampling strategy for the determination of paracetamol in blood and cerebrospinal fluid. Analytical and Bioanalytical Chemistry, 411(1), pp. 181-191. doi:10.1007/s00216-018-1427-6.
Delahaye L, et al. Volumetric Absorptive Microsampling as an Alternative Sampling Strategy for the Determination of Paracetamol in Blood and Cerebrospinal Fluid. Anal Bioanal Chem. 2019;411(1):181-191. PubMed PMID: 30353218.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Volumetric absorptive microsampling as an alternative sampling strategy for the determination of paracetamol in blood and cerebrospinal fluid. AU - Delahaye,Lisa, AU - Dhont,Evelyn, AU - De Cock,Pieter, AU - De Paepe,Peter, AU - Stove,Christophe P, Y1 - 2018/10/23/ PY - 2018/07/04/received PY - 2018/10/10/accepted PY - 2018/09/19/revised PY - 2018/10/26/pubmed PY - 2018/10/26/medline PY - 2018/10/25/entrez KW - Alternative sampling strategies KW - Cerebrospinal fluid KW - Liquid chromatography-tandem mass spectrometry KW - Paracetamol KW - Volumetric absorptive microsampling SP - 181 EP - 191 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 411 IS - 1 N2 - In the field of bioanalysis, dried matrix spot sampling is increasingly receiving interest, as this alternative sampling strategy offers many potential benefits over traditional sampling, including matrix volume-sparing properties. By using a microsampling strategy, e.g., volumetric absorptive microsampling (VAMS), the number of samples that can be collected from a patient can be increased, as a result of the limited sample volume that is required per sample. To date, no VAMS-based methods have been developed for the quantification of analytes in cerebrospinal fluid (CSF). The objective of this study was to develop and validate two LC-MS/MS methods for the quantification of paracetamol in dried blood and dried CSF, with both matrices sampled using VAMS. Both methods were fully validated based on internationally accepted guidelines. Paracetamol was chromatographically separated from its glucuronide and sulfate metabolites and no carry-over or unacceptable interferences were detected. The total precision (%RSD) was below 15% for all QC levels and accuracy (%bias) was below 7% (17% for the LLOQ of aqueous VAMS). The influence of the hematocrit on the recovery of blood VAMS samples appeared to be limited within the hematocrit range of 0.21 to 0.62. The blood VAMS samples were stable for 1 week if stored at 50 °C, and for at least 8 months when stored between - 80 °C and room temperature. The aqueous VAMS samples were stable for at least 9 months when stored between - 80 and 4 °C, and for 1 month when stored at room temperature. Application of the methods on external quality control material and analysis of patient samples demonstrated the validity and utility of the methods and provided a proof of concept for the analysis of CSF microsamples obtained via VAMS devices. Graphical abstract ᅟ. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/30353218/Volumetric_absorptive_microsampling_as_an_alternative_sampling_strategy_for_the_determination_of_paracetamol_in_blood_and_cerebrospinal_fluid_ L2 - https://dx.doi.org/10.1007/s00216-018-1427-6 DB - PRIME DP - Unbound Medicine ER -