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Leaf Extracts of Aerva lanata Inhibit the Activities of Type 2 Diabetes-Related Enzymes and Possess Antioxidant Properties.
Oxid Med Cell Longev. 2018; 2018:3439048.OM

Abstract

The leaves of Aerva lanata are one of the indigenous medicinal plants used in the management of diabetes mellitus and its associated complications in Africa. However, its effect on the activities of diabetes-related enzymes has not been investigated. This study evaluated the in vitro inhibitory effects of different extracts of the A. lanata leaf on the activities of diabetes-related enzymes (α-amylase and α-glucosidase) and chemically induced free radicals. Aqueous, ethanol, and hydroethanol extracts of A. lanata leaves were subjected to a standard enzyme inhibition assay followed by determination of modes of inhibition of the enzymes. The antioxidant activities of the extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The results obtained showed that the hydroethanol extract of the A. lanata leaf optimally inhibited both α-amylase (IC50: 2.42 mg/mL) and α-glucosidase (IC50: 0.23 mg/mL). The Lineweaver-Burk plot revealed that the mode of inhibition of both enzymes by the hydroethanol extract was uncompetitive. However, the hydroethanol and aqueous extracts displayed the best DPPH and ABTS radical-scavenging ability, respectively. It can be concluded that the A. lanata extract inhibited the activities of both α-amylase and α-glucosidase uncompetitively, which may be attributed to its free radical-scavenging properties and rich phenolic composition.

Authors+Show Affiliations

Department of Biochemistry, University of Ilorin, PMB 1515 Ilorin, Nigeria.Department of Biochemistry, Lagos State University, PMB 0001 Ojo, Lagos, Nigeria.Department of Biochemistry, Lagos State University, PMB 0001 Ojo, Lagos, Nigeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30356437

Citation

Akanji, Musbau Adewunmi, et al. "Leaf Extracts of Aerva Lanata Inhibit the Activities of Type 2 Diabetes-Related Enzymes and Possess Antioxidant Properties." Oxidative Medicine and Cellular Longevity, vol. 2018, 2018, p. 3439048.
Akanji MA, Olukolu SO, Kazeem MI. Leaf Extracts of Aerva lanata Inhibit the Activities of Type 2 Diabetes-Related Enzymes and Possess Antioxidant Properties. Oxid Med Cell Longev. 2018;2018:3439048.
Akanji, M. A., Olukolu, S. O., & Kazeem, M. I. (2018). Leaf Extracts of Aerva lanata Inhibit the Activities of Type 2 Diabetes-Related Enzymes and Possess Antioxidant Properties. Oxidative Medicine and Cellular Longevity, 2018, 3439048. https://doi.org/10.1155/2018/3439048
Akanji MA, Olukolu SO, Kazeem MI. Leaf Extracts of Aerva Lanata Inhibit the Activities of Type 2 Diabetes-Related Enzymes and Possess Antioxidant Properties. Oxid Med Cell Longev. 2018;2018:3439048. PubMed PMID: 30356437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leaf Extracts of Aerva lanata Inhibit the Activities of Type 2 Diabetes-Related Enzymes and Possess Antioxidant Properties. AU - Akanji,Musbau Adewunmi, AU - Olukolu,Samson Olasunkanmi, AU - Kazeem,Mutiu Idowu, Y1 - 2018/09/26/ PY - 2018/05/04/received PY - 2018/08/04/revised PY - 2018/08/26/accepted PY - 2018/10/26/entrez PY - 2018/10/26/pubmed PY - 2018/11/27/medline SP - 3439048 EP - 3439048 JF - Oxidative medicine and cellular longevity JO - Oxid Med Cell Longev VL - 2018 N2 - The leaves of Aerva lanata are one of the indigenous medicinal plants used in the management of diabetes mellitus and its associated complications in Africa. However, its effect on the activities of diabetes-related enzymes has not been investigated. This study evaluated the in vitro inhibitory effects of different extracts of the A. lanata leaf on the activities of diabetes-related enzymes (α-amylase and α-glucosidase) and chemically induced free radicals. Aqueous, ethanol, and hydroethanol extracts of A. lanata leaves were subjected to a standard enzyme inhibition assay followed by determination of modes of inhibition of the enzymes. The antioxidant activities of the extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The results obtained showed that the hydroethanol extract of the A. lanata leaf optimally inhibited both α-amylase (IC50: 2.42 mg/mL) and α-glucosidase (IC50: 0.23 mg/mL). The Lineweaver-Burk plot revealed that the mode of inhibition of both enzymes by the hydroethanol extract was uncompetitive. However, the hydroethanol and aqueous extracts displayed the best DPPH and ABTS radical-scavenging ability, respectively. It can be concluded that the A. lanata extract inhibited the activities of both α-amylase and α-glucosidase uncompetitively, which may be attributed to its free radical-scavenging properties and rich phenolic composition. SN - 1942-0994 UR - https://www.unboundmedicine.com/medline/citation/30356437/Leaf_Extracts_of_Aerva_lanata_Inhibit_the_Activities_of_Type_2_Diabetes_Related_Enzymes_and_Possess_Antioxidant_Properties_ L2 - https://doi.org/10.1155/2018/3439048 DB - PRIME DP - Unbound Medicine ER -