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Cytogenetic and genotoxic effects of 2-chlorophenol on Allium cepa L. root meristem cells.
Environ Sci Pollut Res Int. 2018 Dec; 25(36):36117-36123.ES

Abstract

2-Chlorophenol (2-CP), a class of chlorinated organic pollutants like other chlorophenols, is used as intermediate in the synthesis of the higher chlorinated congeners, certain dyes, preservatives, herbicides, fungicides, and plastics. In this study, cytotoxic and genotoxic effects of 2-CP were investigated on the root meristem cells of Allium cepa for its effects on root growth, mitotic index (MI), mitotic phases, chromosomal abnormalities (CAs), and DNA damage by using Allium anaphase-telophase and Comet assays. EC50 of 2-CP value was determined as approximately 25 mg/L by Allium root growth inhibition test. Three concentrations of 2-CP (12.5, 25, and 50 mg/L), distilled water (negative control), and methyl methane sulfonate (MMS, 10 mg/L, positive control) were applied to onion stem cells under different exposure periods (24, 48, 72, and 96 h). All the applied doses of 2-CP slightly decreased MIs. 2-CP induced total CAs such as disturbed anaphase-telophase, chromosome laggards, stickiness, and bridges and also DNA damage at significant levels. These results demonstrate that 2-CP has genotoxic effects in A. cepa root meristematic cells.

Authors+Show Affiliations

Faculty of Arts and Sciences, Molecular Biology and Genetics Department, Uşak University, 1 Eylül Campus, 64300, Uşak, Turkey.Faculty of Arts and Sciences, Molecular Biology and Genetics Department, Uşak University, 1 Eylül Campus, 64300, Uşak, Turkey. rliman@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30357725

Citation

Küçük, Derya, and Recep Liman. "Cytogenetic and Genotoxic Effects of 2-chlorophenol On Allium Cepa L. Root Meristem Cells." Environmental Science and Pollution Research International, vol. 25, no. 36, 2018, pp. 36117-36123.
Küçük D, Liman R. Cytogenetic and genotoxic effects of 2-chlorophenol on Allium cepa L. root meristem cells. Environ Sci Pollut Res Int. 2018;25(36):36117-36123.
Küçük, D., & Liman, R. (2018). Cytogenetic and genotoxic effects of 2-chlorophenol on Allium cepa L. root meristem cells. Environmental Science and Pollution Research International, 25(36), 36117-36123. https://doi.org/10.1007/s11356-018-3502-0
Küçük D, Liman R. Cytogenetic and Genotoxic Effects of 2-chlorophenol On Allium Cepa L. Root Meristem Cells. Environ Sci Pollut Res Int. 2018;25(36):36117-36123. PubMed PMID: 30357725.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytogenetic and genotoxic effects of 2-chlorophenol on Allium cepa L. root meristem cells. AU - Küçük,Derya, AU - Liman,Recep, Y1 - 2018/10/24/ PY - 2018/07/12/received PY - 2018/10/16/accepted PY - 2018/10/26/pubmed PY - 2019/3/19/medline PY - 2018/10/26/entrez KW - 2-Chlorophenol KW - Allium cepa KW - Chromosome aberration KW - Comet assay KW - Genotoxicity KW - Toxicity SP - 36117 EP - 36123 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 25 IS - 36 N2 - 2-Chlorophenol (2-CP), a class of chlorinated organic pollutants like other chlorophenols, is used as intermediate in the synthesis of the higher chlorinated congeners, certain dyes, preservatives, herbicides, fungicides, and plastics. In this study, cytotoxic and genotoxic effects of 2-CP were investigated on the root meristem cells of Allium cepa for its effects on root growth, mitotic index (MI), mitotic phases, chromosomal abnormalities (CAs), and DNA damage by using Allium anaphase-telophase and Comet assays. EC50 of 2-CP value was determined as approximately 25 mg/L by Allium root growth inhibition test. Three concentrations of 2-CP (12.5, 25, and 50 mg/L), distilled water (negative control), and methyl methane sulfonate (MMS, 10 mg/L, positive control) were applied to onion stem cells under different exposure periods (24, 48, 72, and 96 h). All the applied doses of 2-CP slightly decreased MIs. 2-CP induced total CAs such as disturbed anaphase-telophase, chromosome laggards, stickiness, and bridges and also DNA damage at significant levels. These results demonstrate that 2-CP has genotoxic effects in A. cepa root meristematic cells. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/30357725/Cytogenetic_and_genotoxic_effects_of_2_chlorophenol_on_Allium_cepa_L__root_meristem_cells_ L2 - https://dx.doi.org/10.1007/s11356-018-3502-0 DB - PRIME DP - Unbound Medicine ER -