Tags

Type your tag names separated by a space and hit enter

Single probiotic supplement suppresses colitis-associated colorectal tumorigenesis by modulating inflammatory development and microbial homeostasis.
J Gastroenterol Hepatol. 2019 Jul; 34(7):1182-1192.JG

Abstract

BACKGROUND AND AIM

Chronic inflammation is a major contributor to the initiation and progression of cancers. Lactobacillus helveticus NS8, which was originally separated from fermented koumiss, exhibited anti-inflammatory functions in our prior studies. In this study, NS8 was investigated for its potential to prevent colitis-associated colorectal cancer (CAC).

METHODS

The protective effects of NS8 against CAC was explored by employing the azoxymethane plus dextran sodium sulfate-induced carcinogenesis mouse model. The prevalences of T cells expressing specific inflammatory cytokines were measured by flow cytometry at the early stage of CAC. Inflammatory modulation by NS8 was also tested in the Caco2-Raw264.7 cell co-culture system. The alternations in the intestinal microbiota following the health-inflammation-cancer sequence were analyzed by 16S rDNA sequencing.

RESULTS

Oral intake of NS8 lactobacilli clearly reduced tumor number and the degree of hyperplasia. The increased proliferation of enterocytes at the early stage of CAC was significantly suppressed by NS8, while the level of apoptosis was elevated. The anticancer effects of NS8 were associated with its anti-colitis outcomes before tumor formation. NS8 significantly suppressed the activation of NF-κB and upregulated the anti-inflammatory cytokine IL-10. Further analysis revealed the marked downregulation of IL-17-producing T cells by NS8. Furthermore, NS8 modulated intestinal dysbiosis by promoting beneficial commensal microbes while suppressing cancer-associated microbes. Notably, Bacteroides acidifaciens was the most sensitive commensal bacteria to NS8 intervention.

CONCLUSION

These results provide insight into the protective effects of L. helveticus NS8 against colorectal cancer.

Authors+Show Affiliations

Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.School of Medicine, Hangzhou Normal University, Hangzhou, China.School of Medicine, Hangzhou Normal University, Hangzhou, China.School of Medicine, Hangzhou Normal University, Hangzhou, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30357910

Citation

Rong, Jingjing, et al. "Single Probiotic Supplement Suppresses Colitis-associated Colorectal Tumorigenesis By Modulating Inflammatory Development and Microbial Homeostasis." Journal of Gastroenterology and Hepatology, vol. 34, no. 7, 2019, pp. 1182-1192.
Rong J, Liu S, Hu C, et al. Single probiotic supplement suppresses colitis-associated colorectal tumorigenesis by modulating inflammatory development and microbial homeostasis. J Gastroenterol Hepatol. 2019;34(7):1182-1192.
Rong, J., Liu, S., Hu, C., & Liu, C. (2019). Single probiotic supplement suppresses colitis-associated colorectal tumorigenesis by modulating inflammatory development and microbial homeostasis. Journal of Gastroenterology and Hepatology, 34(7), 1182-1192. https://doi.org/10.1111/jgh.14516
Rong J, et al. Single Probiotic Supplement Suppresses Colitis-associated Colorectal Tumorigenesis By Modulating Inflammatory Development and Microbial Homeostasis. J Gastroenterol Hepatol. 2019;34(7):1182-1192. PubMed PMID: 30357910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single probiotic supplement suppresses colitis-associated colorectal tumorigenesis by modulating inflammatory development and microbial homeostasis. AU - Rong,Jingjing, AU - Liu,Shuzhan, AU - Hu,Chao, AU - Liu,Chen, Y1 - 2018/12/06/ PY - 2018/09/24/received PY - 2018/10/15/revised PY - 2018/10/17/accepted PY - 2018/10/26/pubmed PY - 2020/1/30/medline PY - 2018/10/26/entrez KW - IL-17 KW - Lactobacillus helveticus KW - colorectal cancer KW - commensal microbe KW - inflammation KW - microbiota KW - probiotic SP - 1182 EP - 1192 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 34 IS - 7 N2 - BACKGROUND AND AIM: Chronic inflammation is a major contributor to the initiation and progression of cancers. Lactobacillus helveticus NS8, which was originally separated from fermented koumiss, exhibited anti-inflammatory functions in our prior studies. In this study, NS8 was investigated for its potential to prevent colitis-associated colorectal cancer (CAC). METHODS: The protective effects of NS8 against CAC was explored by employing the azoxymethane plus dextran sodium sulfate-induced carcinogenesis mouse model. The prevalences of T cells expressing specific inflammatory cytokines were measured by flow cytometry at the early stage of CAC. Inflammatory modulation by NS8 was also tested in the Caco2-Raw264.7 cell co-culture system. The alternations in the intestinal microbiota following the health-inflammation-cancer sequence were analyzed by 16S rDNA sequencing. RESULTS: Oral intake of NS8 lactobacilli clearly reduced tumor number and the degree of hyperplasia. The increased proliferation of enterocytes at the early stage of CAC was significantly suppressed by NS8, while the level of apoptosis was elevated. The anticancer effects of NS8 were associated with its anti-colitis outcomes before tumor formation. NS8 significantly suppressed the activation of NF-κB and upregulated the anti-inflammatory cytokine IL-10. Further analysis revealed the marked downregulation of IL-17-producing T cells by NS8. Furthermore, NS8 modulated intestinal dysbiosis by promoting beneficial commensal microbes while suppressing cancer-associated microbes. Notably, Bacteroides acidifaciens was the most sensitive commensal bacteria to NS8 intervention. CONCLUSION: These results provide insight into the protective effects of L. helveticus NS8 against colorectal cancer. SN - 1440-1746 UR - https://www.unboundmedicine.com/medline/citation/30357910/Single_probiotic_supplement_suppresses_colitis_associated_colorectal_tumorigenesis_by_modulating_inflammatory_development_and_microbial_homeostasis_ L2 - https://doi.org/10.1111/jgh.14516 DB - PRIME DP - Unbound Medicine ER -