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[Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep by Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats].
Zhen Ci Yan Jiu. 2018 Oct 25; 43(10):651-6.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) stimulation of single and multiple acupoints on sleep and concentrations of interlukin-1 β(IL-1 β), brain-derived neurotrophic factor (BDNF), prostaglandin D2(PGD2) and melatonin (MLT, sleep-promoting factors) and corticosterone (CORT, awakening-promoting factor) in the serum in insomnia rats, so as to explore its efficacy difference and the mechanism underlying improving sleep.

METHODS

Fifty-four male SD rats were randomly divided into control, model, EA-Baihui (GV 20), EA-Shenmen (HT 7), EA-Sanyinjiao (SP 6) and EA-GV 20+HT 7+SP 6 groups (n=9 rats in each group). The insomnia model was established by intraperitoneal injection of para-chlorophenylalanine (PCPA, 300 mg/kg) once daily for 2 days. In the EA-GV 20, EA-HT 7, EA-SP 6 and EA-GV 20+HT 7+SP 6 groups, EA stimulation was administrated for 30 min, once a day for 4 days. The sleep onset latency and sleep duration were measured after intraperitoneal injection of pentobarbital sodium (35 mg/kg). The concentrations of IL-1 β, BDNF, MLT, PGD2and CORT in the serum were detected by ELISA.

RESULTS

After EA stimulation of GV 20, HT 7, SP 6 and GV 20+HT 7+SP 6, the sleep latency was significantly shortened (P<0.05, P<0.01, except SP 6), and the sleep duration was remarkably prolonged in comparison with the model group (P<0.05, P<0.01), and the therapeutic effects of EA-GV 20+HT 7+SP 6 were significantly superior to those of EA-GV 20, EA-HT 7 and EA-SP 6 in shortening the sleep latency and lengthening the sleep duration (P<0.05). Following modeling, the concentrations of IL-1 β, BDNF, PGD2 and MLT were significantly down-regulated, and the CORT level was markedly up-regulated in the model group relevant to the control group (P<0.05). Following EA,modeling induced dramatic decrease of serum IL-1 β, BDNF, PGD2 and MLT was considerably up-regulated, and the increased CORT level markedly down-regulated in the EA-GV 20, EA-HT 7, EA-SP 6 and EA-GV 20+HT 7+SP 6 groups (P<0.05). The effects of EA-GV 20+HT 7+SP 6 were evidently superior to those of EA-GV 20 and EA-SP 6 in up-regulating serum IL-1 β, BDNF and PGD2levels, and to those of HT 7, GV 20 and SP 6 in up-regulating serum MLT level, and significantly superior to those of EA-ST 7 and EA-SP 6 in down-regulating serum CORT (P<0.05).

CONCLUSION

EA stimulation of HT 7, GV 20, SP 6 and GV 20+HT 7+ SP 6 can significantly improve the sleep in insomnia rats, which is closely associated with its effects in regulating serum sleep-promoting factors and awakening-promoting factor. Joint administration of EA of GV 20+HT 7+ SP 6 has a better effect than the single acupoint mentioned above.

Authors+Show Affiliations

Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China; College of Acupuncture-moxibustion and Massage, Changchun University of Traditional Chinese Medicine, Changchun 130117.Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China; College of Acupuncture-moxibustion and Massage, Changchun University of Traditional Chinese Medicine, Changchun 130117.College of Acupuncture-moxibustion and Massage, Changchun University of Traditional Chinese Medicine, Changchun 130117.Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

30365261

Citation

Qiao, Li-Na, et al. "[Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep By Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 43, no. 10, 2018, pp. 651-6.
Qiao LN, Gao QL, Tan LH, et al. [Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep by Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats]. Zhen Ci Yan Jiu. 2018;43(10):651-6.
Qiao, L. N., Gao, Q. L., Tan, L. H., Shi, Y. N., Jiang, Y. S., Yang, J. J., & Yang, Y. S. (2018). [Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep by Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 43(10), 651-6. https://doi.org/10.13702/j.1000-0607.180317
Qiao LN, et al. [Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep By Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats]. Zhen Ci Yan Jiu. 2018 Oct 25;43(10):651-6. PubMed PMID: 30365261.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep by Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats]. AU - Qiao,Li-Na, AU - Gao,Qiao-Ling, AU - Tan,Lian-Hong, AU - Shi,Yi-Nan, AU - Jiang,Yan-Shu, AU - Yang,Jiao-Jiao, AU - Yang,Yong-Sheng, PY - 2018/10/27/entrez PY - 2018/10/27/pubmed PY - 2019/4/9/medline KW - Acupoint combination KW - Awakening-promoting factor KW - Electroacupuncture KW - Insomnia KW - Peripheral blood KW - Sleep-promoting factors SP - 651 EP - 6 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 43 IS - 10 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) stimulation of single and multiple acupoints on sleep and concentrations of interlukin-1 β(IL-1 β), brain-derived neurotrophic factor (BDNF), prostaglandin D2(PGD2) and melatonin (MLT, sleep-promoting factors) and corticosterone (CORT, awakening-promoting factor) in the serum in insomnia rats, so as to explore its efficacy difference and the mechanism underlying improving sleep. METHODS: Fifty-four male SD rats were randomly divided into control, model, EA-Baihui (GV 20), EA-Shenmen (HT 7), EA-Sanyinjiao (SP 6) and EA-GV 20+HT 7+SP 6 groups (n=9 rats in each group). The insomnia model was established by intraperitoneal injection of para-chlorophenylalanine (PCPA, 300 mg/kg) once daily for 2 days. In the EA-GV 20, EA-HT 7, EA-SP 6 and EA-GV 20+HT 7+SP 6 groups, EA stimulation was administrated for 30 min, once a day for 4 days. The sleep onset latency and sleep duration were measured after intraperitoneal injection of pentobarbital sodium (35 mg/kg). The concentrations of IL-1 β, BDNF, MLT, PGD2and CORT in the serum were detected by ELISA. RESULTS: After EA stimulation of GV 20, HT 7, SP 6 and GV 20+HT 7+SP 6, the sleep latency was significantly shortened (P<0.05, P<0.01, except SP 6), and the sleep duration was remarkably prolonged in comparison with the model group (P<0.05, P<0.01), and the therapeutic effects of EA-GV 20+HT 7+SP 6 were significantly superior to those of EA-GV 20, EA-HT 7 and EA-SP 6 in shortening the sleep latency and lengthening the sleep duration (P<0.05). Following modeling, the concentrations of IL-1 β, BDNF, PGD2 and MLT were significantly down-regulated, and the CORT level was markedly up-regulated in the model group relevant to the control group (P<0.05). Following EA,modeling induced dramatic decrease of serum IL-1 β, BDNF, PGD2 and MLT was considerably up-regulated, and the increased CORT level markedly down-regulated in the EA-GV 20, EA-HT 7, EA-SP 6 and EA-GV 20+HT 7+SP 6 groups (P<0.05). The effects of EA-GV 20+HT 7+SP 6 were evidently superior to those of EA-GV 20 and EA-SP 6 in up-regulating serum IL-1 β, BDNF and PGD2levels, and to those of HT 7, GV 20 and SP 6 in up-regulating serum MLT level, and significantly superior to those of EA-ST 7 and EA-SP 6 in down-regulating serum CORT (P<0.05). CONCLUSION: EA stimulation of HT 7, GV 20, SP 6 and GV 20+HT 7+ SP 6 can significantly improve the sleep in insomnia rats, which is closely associated with its effects in regulating serum sleep-promoting factors and awakening-promoting factor. Joint administration of EA of GV 20+HT 7+ SP 6 has a better effect than the single acupoint mentioned above. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/30365261/[Electroacupuncture_of_Multiple_Acupoints_Is_Significantly_Superior_to_That_of_Single_Acupoint_in_Improving_Sleep_by_Regulating_Serum_Sleep_promoting_and_Awakening_promoting_Factors_in_Insomnia_Rats]_ L2 - https://medlineplus.gov/insomnia.html DB - PRIME DP - Unbound Medicine ER -