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Dietary EPA and DHA prevent changes in white adipose tissue omega-3 PUFA and oxylipin content associated with a Fads2 deficiency.
J Nutr Biochem 2019; 63:140-149JN

Abstract

Fatty acid desaturase 2 (Fads2) encodes the delta-6 desaturase (D6D) enzyme, which is rate-limiting for the endogenous production of omega-3 long-chain polyunsaturated fatty acids (LC-PUFA). Numerous studies have reported the cardiometabolic health benefits of omega-3 LC-PUFA. Humans carrying genetic variants in the FADS2 gene have reduced levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), as well as oxylipins, in blood, erythrocytes and white adipose tissue (WAT). Similar findings have been reported in whole-body Fads2-/- mice fed a diet deficient in omega-3 LC-PUFA. The objective of this study was to determine if a diet containing EPA and DHA would prevent the deficiencies in WAT lipid profiles seen in Fads2-/- mice fed a diet containing only ALA. Male C57BL/6 J Fads2-/- and wild type (WT) mice were fed a low fat (7% w/w) diet for 9 weeks containing either flaxseed oil + ARASCO (FD, containing~53% ALA) or menhaden oil (MD, containing~14% EPA and 10% DHA). Fads2-/- mice fed an ALA-enriched diet had reduced body weight, little-to-no omega-3 LC-PUFA and a near complete loss of all omega-3 derived oxylipins in both epididymal and inguinal WAT (P<.05) compared to their WT counterparts, as well as altered expression of key regulators of the fatty acid desaturase pathway. However, Fads2-/- mice fed a diet containing EPA and DHA prevented most of these changes. This study provides evidence that a diet containing EPA and DHA provides a nutritional strategy to prevent alterations in WAT lipid content caused by reduced D6D activity.

Authors+Show Affiliations

Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G2W1, Canada.Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G2W1, Canada.Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G2W1, Canada.Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G2W1, Canada.Division of Nutritional Sciences, University of Illinois, Urbana, IL, USA.Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G2W1, Canada.Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G2W1, Canada. Electronic address: dmutch@uoguelph.ca.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30368227

Citation

Sarr, Ousseynou, et al. "Dietary EPA and DHA Prevent Changes in White Adipose Tissue Omega-3 PUFA and Oxylipin Content Associated With a Fads2 Deficiency." The Journal of Nutritional Biochemistry, vol. 63, 2019, pp. 140-149.
Sarr O, Payne GW, Hucik B, et al. Dietary EPA and DHA prevent changes in white adipose tissue omega-3 PUFA and oxylipin content associated with a Fads2 deficiency. J Nutr Biochem. 2019;63:140-149.
Sarr, O., Payne, G. W., Hucik, B., Abdelmagid, S., Nakamura, M. T., Ma, D. W. L., & Mutch, D. M. (2019). Dietary EPA and DHA prevent changes in white adipose tissue omega-3 PUFA and oxylipin content associated with a Fads2 deficiency. The Journal of Nutritional Biochemistry, 63, pp. 140-149. doi:10.1016/j.jnutbio.2018.09.017.
Sarr O, et al. Dietary EPA and DHA Prevent Changes in White Adipose Tissue Omega-3 PUFA and Oxylipin Content Associated With a Fads2 Deficiency. J Nutr Biochem. 2019;63:140-149. PubMed PMID: 30368227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary EPA and DHA prevent changes in white adipose tissue omega-3 PUFA and oxylipin content associated with a Fads2 deficiency. AU - Sarr,Ousseynou, AU - Payne,George W, AU - Hucik,Barbora, AU - Abdelmagid,Salma, AU - Nakamura,Manabu T, AU - Ma,David W L, AU - Mutch,David M, Y1 - 2018/09/26/ PY - 2018/08/17/received PY - 2018/09/12/revised PY - 2018/09/17/accepted PY - 2018/10/28/pubmed PY - 2018/10/28/medline PY - 2018/10/28/entrez KW - Delta-6 desaturase KW - Eicosanoids KW - Epididymal KW - Fatty acids KW - Low-fat diet SP - 140 EP - 149 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 63 N2 - Fatty acid desaturase 2 (Fads2) encodes the delta-6 desaturase (D6D) enzyme, which is rate-limiting for the endogenous production of omega-3 long-chain polyunsaturated fatty acids (LC-PUFA). Numerous studies have reported the cardiometabolic health benefits of omega-3 LC-PUFA. Humans carrying genetic variants in the FADS2 gene have reduced levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), as well as oxylipins, in blood, erythrocytes and white adipose tissue (WAT). Similar findings have been reported in whole-body Fads2-/- mice fed a diet deficient in omega-3 LC-PUFA. The objective of this study was to determine if a diet containing EPA and DHA would prevent the deficiencies in WAT lipid profiles seen in Fads2-/- mice fed a diet containing only ALA. Male C57BL/6 J Fads2-/- and wild type (WT) mice were fed a low fat (7% w/w) diet for 9 weeks containing either flaxseed oil + ARASCO (FD, containing~53% ALA) or menhaden oil (MD, containing~14% EPA and 10% DHA). Fads2-/- mice fed an ALA-enriched diet had reduced body weight, little-to-no omega-3 LC-PUFA and a near complete loss of all omega-3 derived oxylipins in both epididymal and inguinal WAT (P<.05) compared to their WT counterparts, as well as altered expression of key regulators of the fatty acid desaturase pathway. However, Fads2-/- mice fed a diet containing EPA and DHA prevented most of these changes. This study provides evidence that a diet containing EPA and DHA provides a nutritional strategy to prevent alterations in WAT lipid content caused by reduced D6D activity. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/30368227/Dietary_EPA_and_DHA_prevent_changes_in_white_adipose_tissue_omega_3_PUFA_and_oxylipin_content_associated_with_a_Fads2_deficiency_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(18)30798-8 DB - PRIME DP - Unbound Medicine ER -