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Light regulation of coccolithophore host-virus interactions.
New Phytol. 2019 02; 221(3):1289-1302.NP

Abstract

Viruses that infect photoautotrophs have a fundamental relationship with light, given the need for host resources. We investigated the role of light on Coccolithovirus (EhV) infection of the globally distributed coccolithophore, Emiliania huxleyi. Light was required for EhV adsorption, and viral production was highest when host cultures were maintained in continuous light or at irradiance levels of 150-300 μmol m-2 s-1 . During the early stages of infection, photosynthetic electron transport remained high, while RuBisCO expression decreased concomitant with an induction of the pentose phosphate pathway, the primary source of de novo nucleotides. A mathematical model developed and fitted to the laboratory data supported the hypothesis that EhV replication was controlled by a trade-off between host nucleotide recycling and de novo synthesis, and that photoperiod and photon flux could toggle this switch. Laboratory results supported field observations that light was the most robust driver of EhV replication within E. huxleyi populations collected across a 2000 nautical mile transect in the North Atlantic. Collectively, these findings demonstrate that light can drive host-virus interactions through a mechanistic interplay between host metabolic processes, which serve to structure infection and phytoplankton mortality in the upper ocean.

Authors+Show Affiliations

Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New Brunswick, NJ, 08901, USA.Department of Microbiology, The University of Tennessee, Ken and Blaire Mossman Bldg, 1311 Cumberland Ave #307, Knoxville, TN 37996, USA.Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New Brunswick, NJ, 08901, USA.Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New Brunswick, NJ, 08901, USA.Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New Brunswick, NJ, 08901, USA.Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New Brunswick, NJ, 08901, USA.Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New Brunswick, NJ, 08901, USA.WA Organic and Isotope Geochemistry Centre, School of Earth and Planetary Sciences, Curtin University, Bentley, WA, 6102, Australia.Department of Earth, Atmosphere and Planetary Sciences, MIT, Cambridge, MA, 02139, USA.Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New Brunswick, NJ, 08901, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30368816

Citation

Thamatrakoln, Kimberlee, et al. "Light Regulation of Coccolithophore Host-virus Interactions." The New Phytologist, vol. 221, no. 3, 2019, pp. 1289-1302.
Thamatrakoln K, Talmy D, Haramaty L, et al. Light regulation of coccolithophore host-virus interactions. New Phytol. 2019;221(3):1289-1302.
Thamatrakoln, K., Talmy, D., Haramaty, L., Maniscalco, C., Latham, J. R., Knowles, B., Natale, F., Coolen, M. J. L., Follows, M. J., & Bidle, K. D. (2019). Light regulation of coccolithophore host-virus interactions. The New Phytologist, 221(3), 1289-1302. https://doi.org/10.1111/nph.15459
Thamatrakoln K, et al. Light Regulation of Coccolithophore Host-virus Interactions. New Phytol. 2019;221(3):1289-1302. PubMed PMID: 30368816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Light regulation of coccolithophore host-virus interactions. AU - Thamatrakoln,Kimberlee, AU - Talmy,David, AU - Haramaty,Liti, AU - Maniscalco,Christopher, AU - Latham,Jason R, AU - Knowles,Ben, AU - Natale,Frank, AU - Coolen,Marco J L, AU - Follows,Michael J, AU - Bidle,Kay D, Y1 - 2018/10/08/ PY - 2018/06/28/received PY - 2018/08/20/accepted PY - 2018/10/29/pubmed PY - 2020/1/22/medline PY - 2018/10/29/entrez KW - coccolithophores KW - host-virus interactions KW - light KW - mortality KW - pentose phosphate pathway KW - phytoplankton KW - virus SP - 1289 EP - 1302 JF - The New phytologist JO - New Phytol VL - 221 IS - 3 N2 - Viruses that infect photoautotrophs have a fundamental relationship with light, given the need for host resources. We investigated the role of light on Coccolithovirus (EhV) infection of the globally distributed coccolithophore, Emiliania huxleyi. Light was required for EhV adsorption, and viral production was highest when host cultures were maintained in continuous light or at irradiance levels of 150-300 μmol m-2 s-1 . During the early stages of infection, photosynthetic electron transport remained high, while RuBisCO expression decreased concomitant with an induction of the pentose phosphate pathway, the primary source of de novo nucleotides. A mathematical model developed and fitted to the laboratory data supported the hypothesis that EhV replication was controlled by a trade-off between host nucleotide recycling and de novo synthesis, and that photoperiod and photon flux could toggle this switch. Laboratory results supported field observations that light was the most robust driver of EhV replication within E. huxleyi populations collected across a 2000 nautical mile transect in the North Atlantic. Collectively, these findings demonstrate that light can drive host-virus interactions through a mechanistic interplay between host metabolic processes, which serve to structure infection and phytoplankton mortality in the upper ocean. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/30368816/Light_regulation_of_coccolithophore_host_virus_interactions_ L2 - https://doi.org/10.1111/nph.15459 DB - PRIME DP - Unbound Medicine ER -