Tags

Type your tag names separated by a space and hit enter

Nontargeted Metabolomic Study on Variation of Phenolics in Different Cranberry Cultivars Using UPLC-IM - HRMS.
J Agric Food Chem 2018; 66(46):12206-12216JA

Abstract

The metabolomic profiles of American cranberry (Vaccinium macrocarpon) fruits and their variation among 10 diverse cultivars were investigated by ultraperformance liquid chromatography ion-mobility high-resolution mass spectrometry (UPLC-IM - HRMS). Over 80 metabolites, belonging to various phenolic compound groups, were putatively characterized. An HRMS data matrix consisting of 4778 unique ions across the 10 cultivars was built and analyzed by orthogonal projections to latent structures discriminant analysis (OPLS-DA). The 10 cultivars segregated into 4 clusters on the basis of their metabolomic similarities, which largely reflected their genetic backgrounds. Anthocyanins exhibited the most extensive variations among all the cultivars, reflecting the effects of cranberry breeding selection on fruit color. Flavonols, phenolic acid derivatives, and proanthocyanidins also varied among the different cultivars. The nontargeted metabolomic comparison using multivariate analysis proved to be efficient and robust for determining specific metabolite differences among the cultivars.

Authors+Show Affiliations

U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center , Food Composition and Methods Development Laboratory , Beltsville , Maryland 20705 , United States.Philip E. Marucci Center for Blueberry and Cranberry Research and Extension , Rutgers University , Chatsworth , New Jersey 08019 , United States.Center for Intelligent Chemical Instrumentation, Department of Chemistry & Biochemistry , Ohio University , Athens , Ohio 45701 , United States.U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center , Food Composition and Methods Development Laboratory , Beltsville , Maryland 20705 , United States.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

30371081

Citation

Wang, Yifei, et al. "Nontargeted Metabolomic Study On Variation of Phenolics in Different Cranberry Cultivars Using UPLC-IM - HRMS." Journal of Agricultural and Food Chemistry, vol. 66, no. 46, 2018, pp. 12206-12216.
Wang Y, Vorsa N, Harrington PB, et al. Nontargeted Metabolomic Study on Variation of Phenolics in Different Cranberry Cultivars Using UPLC-IM - HRMS. J Agric Food Chem. 2018;66(46):12206-12216.
Wang, Y., Vorsa, N., Harrington, P. B., & Chen, P. (2018). Nontargeted Metabolomic Study on Variation of Phenolics in Different Cranberry Cultivars Using UPLC-IM - HRMS. Journal of Agricultural and Food Chemistry, 66(46), pp. 12206-12216. doi:10.1021/acs.jafc.8b05029.
Wang Y, et al. Nontargeted Metabolomic Study On Variation of Phenolics in Different Cranberry Cultivars Using UPLC-IM - HRMS. J Agric Food Chem. 2018 Nov 21;66(46):12206-12216. PubMed PMID: 30371081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nontargeted Metabolomic Study on Variation of Phenolics in Different Cranberry Cultivars Using UPLC-IM - HRMS. AU - Wang,Yifei, AU - Vorsa,Nicholi, AU - Harrington,Peter de B, AU - Chen,Pei, Y1 - 2018/11/06/ PY - 2018/10/30/pubmed PY - 2019/1/23/medline PY - 2018/10/30/entrez KW - HRMS KW - OPLS-DA KW - UPLC KW - chemometrics KW - cranberry KW - phenolics SP - 12206 EP - 12216 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 66 IS - 46 N2 - The metabolomic profiles of American cranberry (Vaccinium macrocarpon) fruits and their variation among 10 diverse cultivars were investigated by ultraperformance liquid chromatography ion-mobility high-resolution mass spectrometry (UPLC-IM - HRMS). Over 80 metabolites, belonging to various phenolic compound groups, were putatively characterized. An HRMS data matrix consisting of 4778 unique ions across the 10 cultivars was built and analyzed by orthogonal projections to latent structures discriminant analysis (OPLS-DA). The 10 cultivars segregated into 4 clusters on the basis of their metabolomic similarities, which largely reflected their genetic backgrounds. Anthocyanins exhibited the most extensive variations among all the cultivars, reflecting the effects of cranberry breeding selection on fruit color. Flavonols, phenolic acid derivatives, and proanthocyanidins also varied among the different cultivars. The nontargeted metabolomic comparison using multivariate analysis proved to be efficient and robust for determining specific metabolite differences among the cultivars. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/30371081/Nontargeted_Metabolomic_Study_on_Variation_of_Phenolics_in_Different_Cranberry_Cultivars_Using_UPLC_IM___HRMS_ L2 - https://dx.doi.org/10.1021/acs.jafc.8b05029 DB - PRIME DP - Unbound Medicine ER -