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Brahma-related gene 1 ameliorates the neuronal apoptosis and oxidative stress induced by oxygen-glucose deprivation/reoxygenation through activation of Nrf2/HO-1 signaling.
Biomed Pharmacother. 2018 Dec; 108:1216-1224.BP

Abstract

Accumulating evidence suggests that brahma-related gene 1 (Brg1) is a critical regulator of cell apoptosis and oxidative stress in response to various insults; however, whether Brg1 regulates neuronal apoptosis and oxidative stress during cerebral ischemia/reperfusion injury remains unclear. This study aimed to investigate the expression, biological function, and regulatory mechanism of Brg1 in regulating neuronal apoptosis and oxidative stress induced by oxygen-glucose deprivation/reoxygenation (OGD/R), an in vitro cellular model of cerebral ischemia and reperfusion injury. The results showed that Brg1 expression was altered in response to OGD/R treatment. Overexpression of Brg-1 increased cell viability, attenuated apoptosis, and reduced reactive oxygen species (ROS) production in neurons, thus showing a protective effect against OGD/R-induced injury. In contrast, knockdown of Brg1 significantly inhibited cell viability, increased apoptosis, and promoted ROS production in neurons following OGD/R treatment. Moreover, these results showed that Brg1 promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and increased the expression of heme oxygenase-1 (HO-1). However, knockdown of Nrf2 or HO-1 significantly abrogated Brg1-mediated neuroprotective effects. Taken together, these results demonstrate that Brg1 ameliorates OGD/R-induced neuronal injury in vitro by promoting the activation of Nrf2/HO-1 signaling. The study highlights a potential role of Brg1 in regulating cerebral ischemia/reperfusion injury in vivo.

Authors+Show Affiliations

Anesthesiology Department, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 277 West Yanta Road, Shaanxi Province, 710061, China. Electronic address: doctorlifeng@xjtu.edu.cn.Radiotherapy Department, Shaanxi Provincial Tumor Hospital, Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi Province, 710061, China.Neurosurgery Department, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan Province, 450000, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30372823

Citation

Li, Feng, et al. "Brahma-related Gene 1 Ameliorates the Neuronal Apoptosis and Oxidative Stress Induced By Oxygen-glucose Deprivation/reoxygenation Through Activation of Nrf2/HO-1 Signaling." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 108, 2018, pp. 1216-1224.
Li F, Liang J, Tang D. Brahma-related gene 1 ameliorates the neuronal apoptosis and oxidative stress induced by oxygen-glucose deprivation/reoxygenation through activation of Nrf2/HO-1 signaling. Biomed Pharmacother. 2018;108:1216-1224.
Li, F., Liang, J., & Tang, D. (2018). Brahma-related gene 1 ameliorates the neuronal apoptosis and oxidative stress induced by oxygen-glucose deprivation/reoxygenation through activation of Nrf2/HO-1 signaling. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 108, 1216-1224. https://doi.org/10.1016/j.biopha.2018.09.144
Li F, Liang J, Tang D. Brahma-related Gene 1 Ameliorates the Neuronal Apoptosis and Oxidative Stress Induced By Oxygen-glucose Deprivation/reoxygenation Through Activation of Nrf2/HO-1 Signaling. Biomed Pharmacother. 2018;108:1216-1224. PubMed PMID: 30372823.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Brahma-related gene 1 ameliorates the neuronal apoptosis and oxidative stress induced by oxygen-glucose deprivation/reoxygenation through activation of Nrf2/HO-1 signaling. AU - Li,Feng, AU - Liang,Jing, AU - Tang,Dongfang, Y1 - 2018/10/02/ PY - 2018/05/24/received PY - 2018/09/19/revised PY - 2018/09/26/accepted PY - 2018/10/31/pubmed PY - 2019/3/19/medline PY - 2018/10/31/entrez KW - Brg1 KW - Cerebral ischemia and reperfusion KW - HO-1 KW - Nrf2 KW - OGD/R SP - 1216 EP - 1224 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 108 N2 - Accumulating evidence suggests that brahma-related gene 1 (Brg1) is a critical regulator of cell apoptosis and oxidative stress in response to various insults; however, whether Brg1 regulates neuronal apoptosis and oxidative stress during cerebral ischemia/reperfusion injury remains unclear. This study aimed to investigate the expression, biological function, and regulatory mechanism of Brg1 in regulating neuronal apoptosis and oxidative stress induced by oxygen-glucose deprivation/reoxygenation (OGD/R), an in vitro cellular model of cerebral ischemia and reperfusion injury. The results showed that Brg1 expression was altered in response to OGD/R treatment. Overexpression of Brg-1 increased cell viability, attenuated apoptosis, and reduced reactive oxygen species (ROS) production in neurons, thus showing a protective effect against OGD/R-induced injury. In contrast, knockdown of Brg1 significantly inhibited cell viability, increased apoptosis, and promoted ROS production in neurons following OGD/R treatment. Moreover, these results showed that Brg1 promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and increased the expression of heme oxygenase-1 (HO-1). However, knockdown of Nrf2 or HO-1 significantly abrogated Brg1-mediated neuroprotective effects. Taken together, these results demonstrate that Brg1 ameliorates OGD/R-induced neuronal injury in vitro by promoting the activation of Nrf2/HO-1 signaling. The study highlights a potential role of Brg1 in regulating cerebral ischemia/reperfusion injury in vivo. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/30372823/Brahma_related_gene_1_ameliorates_the_neuronal_apoptosis_and_oxidative_stress_induced_by_oxygen_glucose_deprivation/reoxygenation_through_activation_of_Nrf2/HO_1_signaling_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(18)33532-7 DB - PRIME DP - Unbound Medicine ER -