Tags

Type your tag names separated by a space and hit enter

Protective effects of L-theanine against doxorubicin-induced nephrotoxicity in rats.
Biomed Pharmacother. 2018 Dec; 108:1524-1534.BP

Abstract

BACKGROUND/AIM

L-theanine is the unique amino acid found in tea plants, has antioxidant and antiinflammatory activities, and functions in mental concentration and sleep quality. In this study, it is aimed to investigate the effects of L-theanine on doxorubicin (DOX, a chemotherapeutic agent) induced nephrotoxicity in rats, especially via GSH related enzymes.

MATERIALS AND METHODS

32 male Sprague Dawley rats weighing 300-400 g were randomly assigned into 4 groups (n = 8) and the substances were given intraperitoneally to them: Control group (saline for 5 days); Theanine group (200 mg/kg/day theanine for 5 days); DOX group (single dose of 20 mg/kg DOX); DOX + Theanine group (20 mg/kg DOX at first day and 200 mg/kg/day theanine for 5 days). Kidney tissues were evaluated by histopathological analysis. Serum levels of blood urea nitrogen (BUN) and creatinine by spectrophotometrically; percentage of apoptosis indexes (AI%) in the tissues by TUNEL method; caspase-3 levels, reduced and oxidized glutathione (GSH and GSSG), gamma-glutamyltransferase 1 (GGT1), glutathione reductase (GR), glutathione peroxidase (GPx), and glutathione S-transferase (GST), nuclear factor kappa B p65 (NF-kB p65) by commercial kits; malondialdehyde (MDA) by spectrophotometrically were determined in plasma and kidney tissues.

RESULTS

According to DOX group, the DOX + Theanine group has much lower tissue and plasma GSSG, GGT1, NF-κB p65 levels and tissue AI%, whereas significantly higher GSH levels and GPx, GR, GST activities (P < 0.05).

CONCLUSION

It is suggested that L-theanine may have protective effects by enhancing effects on the antioxidant system of GSH and GSH-related enzymes against DOX-induced nephrotoxicity in rats. But this finding needs to be supported with further studies.

Authors+Show Affiliations

Program of Laborant and Veterinary Health, N.D. Göle Vocational High school, Ardahan University, Ardahan, Turkey. Electronic address: yahyaaltinkaynak@ardahan.edu.tr.Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.Department of Nursing, Faculty of Health, Ardahan University, Ardahan, Turkey.Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.Department of Histology and Embryology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.Department of Pathology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.Department of Histology and Embryology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30372854

Citation

Altınkaynak, Yahya, et al. "Protective Effects of L-theanine Against Doxorubicin-induced Nephrotoxicity in Rats." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 108, 2018, pp. 1524-1534.
Altınkaynak Y, Kural B, Akcan BA, et al. Protective effects of L-theanine against doxorubicin-induced nephrotoxicity in rats. Biomed Pharmacother. 2018;108:1524-1534.
Altınkaynak, Y., Kural, B., Akcan, B. A., Bodur, A., Özer, S., Yuluğ, E., Munğan, S., Kaya, C., & Örem, A. (2018). Protective effects of L-theanine against doxorubicin-induced nephrotoxicity in rats. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 108, 1524-1534. https://doi.org/10.1016/j.biopha.2018.09.171
Altınkaynak Y, et al. Protective Effects of L-theanine Against Doxorubicin-induced Nephrotoxicity in Rats. Biomed Pharmacother. 2018;108:1524-1534. PubMed PMID: 30372854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of L-theanine against doxorubicin-induced nephrotoxicity in rats. AU - Altınkaynak,Yahya, AU - Kural,Birgül, AU - Akcan,Buket A, AU - Bodur,Akın, AU - Özer,Serap, AU - Yuluğ,Esin, AU - Munğan,Sevdegül, AU - Kaya,Cansu, AU - Örem,Asım, Y1 - 2018/10/09/ PY - 2018/08/21/received PY - 2018/09/28/revised PY - 2018/09/28/accepted PY - 2018/10/31/pubmed PY - 2019/3/12/medline PY - 2018/10/31/entrez KW - Antioxidant KW - Apoptosis KW - Glutathione KW - Inflammation KW - L-Theanine SP - 1524 EP - 1534 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed Pharmacother VL - 108 N2 - BACKGROUND/AIM: L-theanine is the unique amino acid found in tea plants, has antioxidant and antiinflammatory activities, and functions in mental concentration and sleep quality. In this study, it is aimed to investigate the effects of L-theanine on doxorubicin (DOX, a chemotherapeutic agent) induced nephrotoxicity in rats, especially via GSH related enzymes. MATERIALS AND METHODS: 32 male Sprague Dawley rats weighing 300-400 g were randomly assigned into 4 groups (n = 8) and the substances were given intraperitoneally to them: Control group (saline for 5 days); Theanine group (200 mg/kg/day theanine for 5 days); DOX group (single dose of 20 mg/kg DOX); DOX + Theanine group (20 mg/kg DOX at first day and 200 mg/kg/day theanine for 5 days). Kidney tissues were evaluated by histopathological analysis. Serum levels of blood urea nitrogen (BUN) and creatinine by spectrophotometrically; percentage of apoptosis indexes (AI%) in the tissues by TUNEL method; caspase-3 levels, reduced and oxidized glutathione (GSH and GSSG), gamma-glutamyltransferase 1 (GGT1), glutathione reductase (GR), glutathione peroxidase (GPx), and glutathione S-transferase (GST), nuclear factor kappa B p65 (NF-kB p65) by commercial kits; malondialdehyde (MDA) by spectrophotometrically were determined in plasma and kidney tissues. RESULTS: According to DOX group, the DOX + Theanine group has much lower tissue and plasma GSSG, GGT1, NF-κB p65 levels and tissue AI%, whereas significantly higher GSH levels and GPx, GR, GST activities (P < 0.05). CONCLUSION: It is suggested that L-theanine may have protective effects by enhancing effects on the antioxidant system of GSH and GSH-related enzymes against DOX-induced nephrotoxicity in rats. But this finding needs to be supported with further studies. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/30372854/Protective_effects_of_L_theanine_against_doxorubicin_induced_nephrotoxicity_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(18)35894-3 DB - PRIME DP - Unbound Medicine ER -