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Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species.
Vet Res. 2018 Oct 29; 49(1):111.VR

Abstract

Digital dermatitis (DD) presents as painful, ulcerative or proliferative lesions that lead to bovine lameness affecting economic efficiency and animal welfare. Although DD etiological agent(s) have not been established, it is widely accepted that DD is a polymicrobial disease significantly associated with species of Treponema and the non-linear disease progression may be attributed to interactions among infecting bacteria. We postulated the morphological changes associated with DD lesion grades are related to interactions among infecting species of Treponema. We developed a novel species-specific qPCR that can identify the absolute abundance of the four of the most common species of Treponema in DD, T. phagedenis, T. medium, T. pedis and T. denticola, in a single reaction. We found species abundance and the number of distinct Treponema species present is higher in active, ulcerative lesions than in healing lesions, chronic lesions, and DD-free skin. Treponema spp. were present in both DD-free skin and M3 lesions following treatment with oxytetracycline. We have also found positive correlations among T. phagedenis, T. medium and T. pedis indicating they are significantly more likely to be found together than apart and their absolute quantities tend to increase together, a relationship which is not present with T. denticola. Further, we found Treponema, particularly viable T. denticola, in lesions 5 days post treatment with oxytetracycline (M3). Our findings suggest that pathogenicity may be closely associated with Treponema abundance, particularly T. phagedenis, T. medium and T. pedis, and interactions among them, independent of T. denticola. Our results provide a novel, consistent method to identify species of Treponema within DD lesions and associate Treponema spp. and abundance with morphological changes related to host pathogenicity.

Authors+Show Affiliations

Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.Department of Animal Science, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, MB, Canada.Department of Animal Science, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, MB, Canada.Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada. jdebuck@ucalgary.ca.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30373670

Citation

Beninger, Caroline, et al. "Associations Between Digital Dermatitis Lesion Grades in Dairy Cattle and the Quantities of Four Treponema Species." Veterinary Research, vol. 49, no. 1, 2018, p. 111.
Beninger C, Naqvi SA, Naushad S, et al. Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species. Vet Res. 2018;49(1):111.
Beninger, C., Naqvi, S. A., Naushad, S., Orsel, K., Luby, C., Derakhshani, H., Khafipour, E., & De Buck, J. (2018). Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species. Veterinary Research, 49(1), 111. https://doi.org/10.1186/s13567-018-0605-z
Beninger C, et al. Associations Between Digital Dermatitis Lesion Grades in Dairy Cattle and the Quantities of Four Treponema Species. Vet Res. 2018 Oct 29;49(1):111. PubMed PMID: 30373670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species. AU - Beninger,Caroline, AU - Naqvi,Syed Ali, AU - Naushad,Sohail, AU - Orsel,Karin, AU - Luby,Chris, AU - Derakhshani,Hooman, AU - Khafipour,Ehsan, AU - De Buck,Jeroen, Y1 - 2018/10/29/ PY - 2018/07/20/received PY - 2018/09/11/accepted PY - 2018/10/31/entrez PY - 2018/10/31/pubmed PY - 2018/11/20/medline SP - 111 EP - 111 JF - Veterinary research JO - Vet. Res. VL - 49 IS - 1 N2 - Digital dermatitis (DD) presents as painful, ulcerative or proliferative lesions that lead to bovine lameness affecting economic efficiency and animal welfare. Although DD etiological agent(s) have not been established, it is widely accepted that DD is a polymicrobial disease significantly associated with species of Treponema and the non-linear disease progression may be attributed to interactions among infecting bacteria. We postulated the morphological changes associated with DD lesion grades are related to interactions among infecting species of Treponema. We developed a novel species-specific qPCR that can identify the absolute abundance of the four of the most common species of Treponema in DD, T. phagedenis, T. medium, T. pedis and T. denticola, in a single reaction. We found species abundance and the number of distinct Treponema species present is higher in active, ulcerative lesions than in healing lesions, chronic lesions, and DD-free skin. Treponema spp. were present in both DD-free skin and M3 lesions following treatment with oxytetracycline. We have also found positive correlations among T. phagedenis, T. medium and T. pedis indicating they are significantly more likely to be found together than apart and their absolute quantities tend to increase together, a relationship which is not present with T. denticola. Further, we found Treponema, particularly viable T. denticola, in lesions 5 days post treatment with oxytetracycline (M3). Our findings suggest that pathogenicity may be closely associated with Treponema abundance, particularly T. phagedenis, T. medium and T. pedis, and interactions among them, independent of T. denticola. Our results provide a novel, consistent method to identify species of Treponema within DD lesions and associate Treponema spp. and abundance with morphological changes related to host pathogenicity. SN - 1297-9716 UR - https://www.unboundmedicine.com/medline/citation/30373670/Associations_between_digital_dermatitis_lesion_grades_in_dairy_cattle_and_the_quantities_of_four_Treponema_species_ L2 - https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-018-0605-z DB - PRIME DP - Unbound Medicine ER -