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S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Petunia Pollen.
Plant Cell. 2018 12; 30(12):2959-2972.PC

Abstract

Self-incompatibility (SI) in Petunia is regulated by a polymorphic S-locus. For each S-haplotype, the S-locus contains a pistil-specific S-RNase gene and multiple pollen-specific S-locus F-box (SLF) genes. Both gain-of-function and loss-of-function experiments have shown that S-RNase alone regulates pistil specificity in SI. Gain-of-function experiments on SLF genes suggest that the entire suite of encoded proteins constitute the pollen specificity determinant. However, clear-cut loss-of-function experiments must be performed to determine if SLF proteins are essential for SI of pollen. Here, we used CRISPR/Cas9 to generate two frame-shift indel alleles of S2 -SLF1 (SLF1 of S2 -haplotype) in S2S3 plants of P. inflata and examined the effect on the SI behavior of S2 pollen. In the absence of a functional S2-SLF1, S2 pollen was either rejected by or remained compatible with pistils carrying one of eight normally compatible S-haplotypes. All results are consistent with interaction relationships between the 17 SLF proteins of S2 -haplotype and these eight S-RNases that had been determined by gain-of-function experiments performed previously or in this work. Our loss-of-function results provide definitive evidence that SLF proteins are solely responsible for SI of pollen, and they reveal their diverse and complex interaction relationships with S-RNases to maintain SI while ensuring cross-compatibility.

Authors+Show Affiliations

Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802.Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802.Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802.Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802.Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802.Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802.Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802.Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802 txk3@psu.edu. Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30377238

Citation

Sun, Linhan, et al. "S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Petunia Pollen." The Plant Cell, vol. 30, no. 12, 2018, pp. 2959-2972.
Sun L, Williams JS, Li S, et al. S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Petunia Pollen. Plant Cell. 2018;30(12):2959-2972.
Sun, L., Williams, J. S., Li, S., Wu, L., Khatri, W. A., Stone, P. G., Keebaugh, M. D., & Kao, T. H. (2018). S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Petunia Pollen. The Plant Cell, 30(12), 2959-2972. https://doi.org/10.1105/tpc.18.00615
Sun L, et al. S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Petunia Pollen. Plant Cell. 2018;30(12):2959-2972. PubMed PMID: 30377238.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Petunia Pollen. AU - Sun,Linhan, AU - Williams,Justin S, AU - Li,Shu, AU - Wu,Lihua, AU - Khatri,Wasi A, AU - Stone,Patrick G, AU - Keebaugh,Matthew D, AU - Kao,Teh-Hui, Y1 - 2018/10/29/ PY - 2018/08/15/received PY - 2018/10/09/revised PY - 2018/10/25/accepted PY - 2018/11/1/pubmed PY - 2019/8/7/medline PY - 2018/11/1/entrez SP - 2959 EP - 2972 JF - The Plant cell JO - Plant Cell VL - 30 IS - 12 N2 - Self-incompatibility (SI) in Petunia is regulated by a polymorphic S-locus. For each S-haplotype, the S-locus contains a pistil-specific S-RNase gene and multiple pollen-specific S-locus F-box (SLF) genes. Both gain-of-function and loss-of-function experiments have shown that S-RNase alone regulates pistil specificity in SI. Gain-of-function experiments on SLF genes suggest that the entire suite of encoded proteins constitute the pollen specificity determinant. However, clear-cut loss-of-function experiments must be performed to determine if SLF proteins are essential for SI of pollen. Here, we used CRISPR/Cas9 to generate two frame-shift indel alleles of S2 -SLF1 (SLF1 of S2 -haplotype) in S2S3 plants of P. inflata and examined the effect on the SI behavior of S2 pollen. In the absence of a functional S2-SLF1, S2 pollen was either rejected by or remained compatible with pistils carrying one of eight normally compatible S-haplotypes. All results are consistent with interaction relationships between the 17 SLF proteins of S2 -haplotype and these eight S-RNases that had been determined by gain-of-function experiments performed previously or in this work. Our loss-of-function results provide definitive evidence that SLF proteins are solely responsible for SI of pollen, and they reveal their diverse and complex interaction relationships with S-RNases to maintain SI while ensuring cross-compatibility. SN - 1532-298X UR - https://www.unboundmedicine.com/medline/citation/30377238/S_Locus_F_Box_Proteins_Are_Solely_Responsible_for_S_RNase_Based_Self_Incompatibility_of_Petunia_Pollen_ DB - PRIME DP - Unbound Medicine ER -